Conventional- and RHPLC-systems in one platform

Conventional- and RHPLC-systems in one platform

The LC-4000 Series RHPLC system with maximum operating pressure 700bar, 70MPa) has been designed to future-proof your HPLC requirements.

Integrated HPLC and UHPLC Systems

The system can be used with conventional 3 – 5 μm particle size analytical columns at typical lower pressures around 10 to 20 MPa (1500 to 3000 psi) and can also be used with smaller particles such as shorter length UHPLC columns and superficially porous (SPP) or Coreshell that require slightly higher pressure solvent delivery to provide optimum linear velocity through the column.
  • ● Up to 700 bar
  • ● Each system component is completely stackable into a modest height
  • ● The system is fully integrated with complete system monitoring
  • ● All user replaceable components are accessible through the front panel
  • ● Vertical leak/drain system to channel away solvent
  • ● Integrated earthquake securing option
  • ● All detectors include 100 Hz acquistion with interchangeable flowcells

Product Specialist

Mats Grapne Kovalent

Mats Grapne

"More than 30 years experience in the field of HPLC, Spectroscopy and other analytical equipments. Contact me if you have questions or need a quotation"
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08-473 3340
  • Advances in Solvent Delivery

    For more than three decades JASCO analytical HPLC pumps have employed an asymmetric twin-piston solvent delivery system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure profiles than other conventional twin-piston reciprocating designs. Slow-Suction Quick-Delivery (SSQD) has been re-developed, with a completely new solvent delivery mechanism ExReFT (Extremely Reliable Flow Technology) offering the highest stability ‘compensated’ solvent delivery used in the PU-4180/85 RHPLC  pump models.
  • Pioneering Optical Design

    As a pioneer in optical spectroscopy, JASCO has optical designs that few can match. We have developed a range of HPLC detectors with unrivaled performance, like the class leading FP-4020 fluorescence detector (water Raman S/N > 2300:1), RI-4035 UHPLC refractive index detector and the unique CD-4095 circular dichroism detector for chiral chromatography.
  • Method Development

    The range of separation columns becoming increasingly diverse – the traditional 5μm fully porous particle is finally losing ground to some of the newer technologies such as the new superficially porous solid core (Coreshell) particles. Requirements for method development are becoming more demanding and time consuming. For method development the columns ovens can accommodate up to 6 or 10 analytical  columns with automatic switching, and 6 or 10 solvent selection valves, controlled by the ChromNAV Method Development Module.
  • Compact and Easy to Use

    The footprint of the LC-4000 Series HPLC is only 30cm – requiring only a very small amount of bench space. For easy maintenance, all LC-4000 instruments feature front-panel access for replacing consumables such as pump components and light sources. The autosampler consumables such as the syringe parts are easy to access.
  • Front Panel Control

    For users requiring front-panel control, the LC-4000 Series retains a keypad and backlit LED display. Keypad control can still be configured for convenient override when under PC control.


HPLC, RHPLC PU-4180, Analytical Pump


Options: Isocratic, Binary (two pumps and high pressure mixer) and  Quaternary (four solvent gradient mixer with degasser)
Flow Rate: Up to 5 mL/min gradient (10mL/min isocratic)
Operating Pressure: Up to 700 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
RHPLC, PU-4185 Semi-Micro Pump

RHPLC Semi- Micro Pumps

Options: Isocratic, (two pumps and high pressure mixer)
Flow Rate: Up to 4 mL/min
Operating Pressure: Up to 700 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
  • Configuration

    The unique expansion bays in the pump base can be fitted with plug-in modules to quickly transform a simple isocratic pumping system into a multi-solvent delivery system. Install a second pump (with dynamic mixer) for binary gradients or a quaternary low pressure mixing gradient unit (four solvent), either can be configured with an optional low volume vacuum degasser.  A 6 – or 10 solvent selection valve can be added for method development.

  • Control

    The PU-4000 Series pumps are normally used as part of a complete HPLC system, however for applications that require stand-alone operation, each instrument has its own keypad and can be controlled using an RS232 protocol and/or contact closures (TTL) to start and stop the pump.

Retention Time Reproducibility

Isocratic Retention time Reproducibility – PU-4180

Isocratic retention time reproducibility and flow accuracy- PU-4180 /1 ml/min)


Mobile Phase  Acetonitrile/Water (80:20) 
Flow Rate  1.0mL/min
Column  CrestPak C18S (4.6mml.D. x 150mm, 5µm)
Column Temperature  40° C
Detection Wave Length  260nm, Response; 1sec
Sample  Polycyclic aromatic hydrocarbon (10µg/mL each)
(Naphthalene, Fluorene, Anthracene, Pyrene, Chrysene, Benzon[a]pyrene)
Injection  10 µL

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At Kovalent we strive to establish and develop long-term partnerships with our customers. When we use the word “partnership” we mean that Kovalent becomes an integral part of our customers’ projects, where we are expected to fully understand their application needs. We aim to make Kovalent our customers’ first and natural choice, and this ambition permeates our entire organisation from initial contact to finished and delivered product.


Kovalent AB
Hulda Lindgrens gata 6A
Phone: +46(0)31-709 81 80
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Org.nr: 556417-2194


Genetec is a member of the same family of companies as Kovalent. Both companies share the same set of values and are in many ways integrated with each other. The main difference between the two companies are their products. Kovalent is involved in system solutions including instrumentation and Genetec is mainly working with consumables. The focus of Genetec is chromatography accessories. You are welcome to visit their state of the art web shop: genetec.se