The system can be used with conventional 3 – 5 μm particle size analytical columns at typical lower pressures around 10 to 20 MPa (1500 to 3000 psi) and can also be used with smaller particles such as shorter length UHPLC columns and superficially porous (SPP) or Coreshell that require slightly higher pressure solvent delivery to provide optimum linear velocity through the column.
● Up to 700 bar
● Each system component is completely stackable into a modest height
● The system is fully integrated with complete system monitoring
● All user replaceable components are accessible through the front panel
● Vertical leak/drain system to channel away solvent
● Integrated earthquake securing option
● All detectors include 100 Hz acquistion with interchangeable flowcells
Product Specialist
Mats Grapne
"More than 30 years experience in the field of HPLC, Spectroscopy and other analytical equipments. Contact me if you have questions or need a quotation"
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08-473 3340
Advances in Solvent Delivery
For more than three decades JASCO analytical HPLC pumps have employed an asymmetric twin-piston solvent delivery system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure profiles than other conventional twin-piston reciprocating designs. Slow-Suction Quick-Delivery (SSQD) has been re-developed, with a completely new solvent delivery mechanism ExReFT (Extremely Reliable Flow Technology) offering the highest stability ‘compensated’ solvent delivery used in the PU-4180/85 RHPLC pump models.
Pioneering Optical Design
As a pioneer in optical spectroscopy, JASCO has optical designs that few can match. We have developed a range of HPLC detectors with unrivaled performance, like the class leading FP-4020 fluorescence detector (water Raman S/N > 2300:1), RI-4035 UHPLC refractive index detector and the unique CD-4095 circular dichroism detector for chiral chromatography.
Method Development
The range of separation columns becoming increasingly diverse – the traditional 5μm fully porous particle is finally losing ground to some of the newer technologies such as the new superficially porous solid core (Coreshell) particles. Requirements for method development are becoming more demanding and time consuming. For method development the columns ovens can accommodate up to 6 or 10 analytical columns with automatic switching, and 6 or 10 solvent selection valves, controlled by the ChromNAV Method Development Module.
Compact and Easy to Use
The footprint of the LC-4000 Series HPLC is only 30cm – requiring only a very small amount of bench space. For easy maintenance, all LC-4000 instruments feature front-panel access for replacing consumables such as pump components and light sources. The autosampler consumables such as the syringe parts are easy to access.
Front Panel Control
For users requiring front-panel control, the LC-4000 Series retains a keypad and backlit LED display. Keypad control can still be configured for convenient override when under PC control.
RHPLC Pumps
HPLC, RHPLC PU-4180, Analytical Pump
RHPLC Pumps
Options: Isocratic, Binary (two pumps and high pressure mixer) and Quaternary (four solvent gradient mixer with degasser) Flow Rate: Up to 5 mL/min gradient (10mL/min isocratic) Operating Pressure: Up to 700 Bar Degassing: Vacuum (Option) Solvents: 2, 6 or 10
RHPLC, PU-4185 Semi-Micro Pump
RHPLC Semi- Micro Pumps
Options: Isocratic, (two pumps and high pressure mixer) Flow Rate: Up to 4 mL/min Operating Pressure: Up to 700 Bar Degassing: Vacuum (Option) Solvents: 2, 6 or 10
Configuration
The unique expansion bays in the pump base can be fitted with plug-in modules to quickly transform a simple isocratic pumping system into a multi-solvent delivery system. Install a second pump (with dynamic mixer) for binary gradients or a quaternary low pressure mixing gradient unit (four solvent), either can be configured with an optional low volume vacuum degasser. A 6 – or 10 solvent selection valve can be added for method development.
Control
The PU-4000 Series pumps are normally used as part of a complete HPLC system, however for applications that require stand-alone operation, each instrument has its own keypad and can be controlled using an RS232 protocol and/or contact closures (TTL) to start and stop the pump.
Retention Time Reproducibility
Isocratic Retention time Reproducibility – PU-4180
Isocratic retention time reproducibility and flow accuracy- PU-4180 /1 ml/min)