Jasco Preparative HPLC

Preparative HPLC-systems for separation on 2.1- 5 cm columns

The LC-4000 Series preparative modules for separation from mg to g scale.

Integrated Preparative HPLC Systems

Suitable for sample clean-up from mg to g scale. The LC-4000 Preparative HPLC comes with a large column oven for multiple preparative columns and optional automatic column switching, open-bed fraction collector with sample trays for tubes, plates or large containers and ChromNAV-FC for fraction control using time, threshold or slope from up to 4 different signals.
  • ● Up to 120 ml/min
  • ● Each system component is completely stackable into a modest height
  • ● The system is fully integrated with complete system monitoring
  • ● All user replaceable components are accessible through the front panel
  • ● Vertical leak/drain system to channel away solvent
  • ● Peak collection by time or peak reqognition
  • ● All detectors include 100 Hz acquistion with interchangeable flowcells

Product Specialist

Mats Grapne

"More than 30 years experience in the field of HPLC, Spectroscopy and other analytical equipments. Contact me if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
08-473 3340
  • Advances in Solvent Delivery

    For more than three decades JASCO analytical HPLC pumps have employed an asymmetric twin-piston solvent delivery system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure profiles than other conventional twin-piston reciprocating designs. Slow-Suction Quick-Delivery (SSQD) has been re-developed, with a completely new solvent delivery mechanism ExReFT (Extremely Reliable Flow Technology) offering the highest stability ‘compensated’ solvent delivery used in the PU-4180/85 RHPLC  pump models.
  • Pioneering Optical Design

    As a pioneer in optical spectroscopy, JASCO has optical designs that few can match. We have developed a range of HPLC detectors with unrivaled performance, like the class leading FP-4020 fluorescence detector (water Raman S/N > 2300:1), RI-4035 UHPLC refractive index detector and the unique CD-4095 circular dichroism detector for chiral chromatography.
  • Compact and Easy to Use

    The footprint of the LC-4000 Series HPLC is only 30cm – requiring only a very small amount of bench space. For easy maintenance, all LC-4000 instruments feature front-panel access for replacing consumables such as pump components and light sources. The autosampler consumables such as the syringe parts are easy to access.
  • Front Panel Control

    For users requiring front-panel control, the LC-4000 Series retains a keypad and backlit LED display. Keypad control can still be configured for convenient override when under PC control.

Preparative HPLC Pumps

HPLC PU-4086, Semi-preparative Pump
Options: Isocratic, Binary (two pumps and high pressure mixer)
Flow Rate: Up to 20 mL/min
Operating Pressure: Up to 500 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
HPLC PU-4087, Preparative Pump
Options: Isocratic, (two pumps and high pressure mixer)
Flow Rate: Up to 50 mL/min
Operating Pressure: Up to 500 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
HPLC PU-4088, Preparative Pump
Options: Isocratic, (two pumps and high pressure mixer)
Flow Rate: Up to 120 mL/min
Operating Pressure: Up to 400 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
  • Configuration

    The unique expansion bays in the pump base can be fitted with plug-in modules to quickly transform a simple isocratic pumping system into a multi-solvent delivery system. Install a second pump (with dynamic mixer) for binary gradients or a quaternary low pressure mixing gradient unit (four solvent), either can be configured with an optional low volume vacuum degasser.  A 6 – or 10 solvent selection valve can be added for method development.

  • Control

    The PU-4000 Series pumps are normally used as part of a complete HPLC system, however for applications that require stand-alone operation, each instrument has its own keypad and can be controlled using an RS232 protocol and/or contact closures (TTL) to start and stop the pump.

Retention Time Reproducibility

Isocratic Retention time Reproducibility – PU-4180

Isocratic retention time reproducibility and flow accuracy- PU-4180 /1 ml/min)


Mobile Phase  Acetonitrile/Water (80:20) 
Flow Rate  1.0mL/min
Column  CrestPak C18S (4.6mml.D. x 150mm, 5µm)
Column Temperature  40° C
Detection Wave Length  260nm, Response; 1sec
Sample  Polycyclic aromatic hydrocarbon (10µg/mL each)
(Naphthalene, Fluorene, Anthracene, Pyrene, Chrysene, Benzon[a]pyrene)
Injection  10 µL


At Kovalent we strive to establish and develop long-term partnerships with our customers. When we use the word “partnership” we mean that Kovalent becomes an integral part of our customers’ projects, where we are expected to fully understand their application needs. We aim to make Kovalent our customers’ first and natural choice, and this ambition permeates our entire organisation from initial contact to finished and delivered product.


Kovalent AB
Hulda Lindgrens gata 6A
Phone: +46(0)31-709 81 80
This email address is being protected from spambots. You need JavaScript enabled to view it.
Org.nr: 556417-2194


Genetec is a member of the same family of companies as Kovalent. Both companies share the same set of values and are in many ways integrated with each other. The main difference between the two companies are their products. Kovalent is involved in system solutions including instrumentation and Genetec is mainly working with consumables. The focus of Genetec is chromatography accessories. You are welcome to visit their state of the art web shop: genetec.se