The MOSH/MOAH-System consists of a binary HPLC system, a GC including two FIDs, an evaluation software, CHRONECT Robotic constisting of a PAL3 Autosampler by CTC Analytics and the software platform CHRONOS by Axel Semrau. The key to success are following technical details:
- Intelligent valve connection: Holds back the MOSH fraction eluted first without losses until the MOAH fraction enters the GC. Then, sample injection to the GC is performed.
- Large-volume injection: The injection into the GC is performed by independent large-volume injection systems. Injection volumes up to 450 µL can be processed smoothly.
- 2-Channel-GC: Two parallel and orthogonal chromatographic separations with subsequent FID detections are performed. Consequently, the results concerning MOSH and MOAH contents in a sample are readily available after 30 minutes only.
- CHRONOS software: The software platform CHRONOS controls sampling, LC and GC including the valve connection and thus constitutes a completely automated control of the method and sample preparation.
This requirements for such a system were developed in close collaboration with end users. Using the CHRONECT LC-GC interface by Axel Semrau the usage of various gas chromatographs is possible. Concerning HPLC, the system may be configured either with a HPLC of KNAUER, Agilent or Shimadzu.