Robust pump solutions from UHPLC to preparative clean-up in gram scale

Jasco LC-4000 series HPLC Pumps


Robust pump solutions from UHPLC to preparative clean-up in gram scale

The LC-4000 Series HPLC pumps have 3 operating pressures RHPLC – rapid (700bar), UHPLC – ultra high performance (1300 bar) and preparative (500 bar). Each base has two expansion bays that allow an isocratic pump to be transformed into a powerful gradient solvent delivery system with optional solvent selection for method development.

HPLC Pumps

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Jasco LC-4000 series HPLC Pumps

RHPLC Pumps

HPLC, RHPLC PU-4180, Analytical Pump

Options: Isocratic, Binary (two pumps and high pressure mixer) and  Quaternary (four solvent gradient mixer with degasser)
Flow Rate: Up to 5 mL/min gradient (10mL/min isocratic)
Operating Pressure: Up to 700 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10

RHPLC Semi- Micro Pumps

RHPLC, PU-4185 Semi-Micro Pump

Options: Isocratic, (two pumps and high pressure mixer)
Flow Rate: Up to 4 mL/min
Operating Pressure: Up to 700 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10

UHPLC Pumps

UHPLC, PU-4285 Semi-Micro Pump

Options: Isocratic, Binary (two pumps and high pressure mixer)
Flow Rate: Up to 2 mL/min gradient (10mL/min isocratic)
Operating Pressure: Up to 1,300 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10

Preparative Pumps

PREPARATIVE, PU-4086/87/88 preparative pumps

Options: Isocratic, (two pumps and high pressure mixer)
Flow Rate: Up to 20 mL/min (PU-4086)
Flow Rate: Up to 50 mL/min (PU-4087)
Flow Rate: Up to 150 mL/min (PU-4088)
Operating Pressure: Up to 500 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
  • Configuration

    The unique expansion bays in the pump base can be fitted with plug-in modules to quickly transform a simple isocratic pumping system into a multi-solvent delivery system. Install a second pump (with dynamic mixer) for binary gradients or a quaternary low pressure mixing gradient unit (four solvent), either can be configured with an optional low volume vacuum degasser.  A 6 – or 10 solvent selection valve can be added for method development.
  • Control

    The PU-4000 Series pumps are normally used as part of a complete HPLC system, however for applications that require stand-alone operation, each instrument has its own keypad and can be controlled using an RS232 protocol and/or contact closures (TTL) to start and stop the pump.

Retention Time Reproducibility

Isocratic Retention time Reproducibility – PU-4180

Isocratic retention time reproducibility and flow accuracy- PU-4180 /1 ml/min)

Conditions

Mobile Phase  Acetonitrile/Water (80:20) 
Flow Rate  1.0mL/min
Column  CrestPak C18S (4.6mml.D. x 150mm, 5µm)
Column Temperature  40° C
Detection Wave Length  260nm, Response; 1sec
Sample  Polycyclic aromatic hydrocarbon (10µg/mL each)
(Naphthalene, Fluorene, Anthracene, Pyrene, Chrysene, Benzon[a]pyrene)
Injection  10 µL

Flexible and robust with many features

Jasco LC-4000 series Autosamplers


Flexible and robust with many features

The LC-4000 Series HPLC autosamplers provide a range of options to suit any sample injection requirements. Any of these products can be added to an existing system with keypad or optional RS232 control.

HPLC Autosamplers

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Jasco LC-4000 series Autosamplers

AS-4050

AS-4050 Routine HPLC Autosampler

The AS-4050 is a 60 sample autosampler for use with conventional HPLC with options for analytical and semi-preparative injection volumes.Up to 300 bar.

AS-4150

AS-4150 RHPLC Autosampler

The AS-4150 is a high-capacity, high performance autosampler designed for RHPLC (up to 700 bar) , with a minimum injection cycle-time of only 30 seconds.

AS-4250

AS-4250 UHPLC Autosampler

The  AS-4250 is high-capacity, high performance autosampler designed for UHPLC (up to 1300 bar), with a minimum injection cycle-time of only 30 seconds.

AS-4058

AS-4058 Preparative Autosampler

The AS-4058 is a large volume injection autosampler, up to 5mL as standard (or 10mL, option). This autosampler is used in preparative systems with either PU-4087 (50mL/min) or PU-4088 (150mL/min) pumps.

  • Sample Protection

    Peltier control is available for maintaining sample temperature and light protection is available on the higher level models for photo-labile samples.

  • Sample Derivatization and Dilution

    Sample preparation is available on the RHPLC and UHPLC autosamplers with programmable sample dilution and pre-column derivitization.

Injection Modes

The injection mode can be switched between full loop and partial loop injections.

Low Carry-Over

An external single solvent wash is included on all models. An optional 5 solvent flushing system can be added to virtually eliminate carry-over. An optional degasser can be installed for degassing up to 5 flushing solvents.

Flow Diagram (Loop-injection)

Maintenance

All LC-4000 Series HPLC autosamplers share the same simple maintenance features, with consumable parts such as injection ports, rotor seals, and loops accessible from the front panel for easy replacement.

Easy maintenance, access all consumable parts through the front panel

Pioneering Optical Design

Jasco LC-4000 series Detectors


UV-visible

Pioneering Optical Design

The UV-4070 (190 – 900nm)  and UV-4075 (190 – 700nm) UV-visible HPLC detectors both use an advanced Czerny-Turner monochromator with dual wavelength and scanning functions. Baseline change due to refractive index is minimized by the flow cell design, and fluctuation from changes in environmental and lamp temperature are reduced with a regulated lamp housing and temperature-controlled cell.

Optional flow cells

Semi-micro, semi-micro SP (UHPLC), preparative and high pressure for SFC

UV-4070/4075 detectors

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Spectral Scanning and Dual Wavelength Detection

Both detectors include dual-wavelength data acquisition (up to 100Hz for UHPLC) and spectral scanning. Spectral scanning can be executed automatically in ChromNAV CDS, by time or by height when a peak exceeds a set threshold.

Easy User Maintenance

User maintenance can be done without removing the module from the system. Light source replacement is made through the front panel and automatic wavelength calibration can be carried out using a mercury lamp

Operation as a Stand-Alone Instrument

The UV-4000 series detectors have a keypad for direct control, parameters can be easily changed and operated in a time program for control throughout a separation (this can be be triggered using a TTL pulse or contact closure). A simple RS232 protocol can be used for control through third part programs.

Easy maintenance, access all consumable parts through the front panel

PDA

High Sensitivity and Flexibility

The LC-4000 Series includes three PDA detectors. The MD-4017 is a simple replacement for a single wavelength detector, the MD-4015 is the workhorse for UV-Visible detection in the range 200 to 600nm and the MD-4010 has a wide wavelength range.

Optional flow cells

Semi-micro, semi-micro SP (UHPLC), preparative and high pressure for SFC

MD-4010/4015/4017

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MD-4010

Light source: D2 and Wl
Wavelenght range: 190-900 nm
PDA elemens: 1024
Noise level: +/- 3,0 x 10-6 Abs
Data Aquisition range: Maximum 100 spectra/s

The MD-4010 PDA detector has high sensitivity, comparable to a single wavelength detector. It uses a 1024 element photometric diode array, with measurement wavelength range from 190nm to 900nm and resolution of 1nm. Fast spectral acquisition rate of up to 100 spectra/sec. The temperature controlled SP type semi-micro flow cell is used narrow peaks typically found with fast UHPLC or RHPLC separations.  An integrated mercury lamp is used for automatic wavelength calibration and validation.

MD-4015

Light source: D2
Wavelenght range: 200-600 nm
PDA elemens: 512
Noise level: +/- 3,0 x 10-6 Abs
Data Aquisition range: Maximum 100 spectra/s

The MD-4015 PDA detector is similar to the MD-4010 with equivalent sensitivity, covering the wavelength range from 200nm to 650nm using a 512 element PDA for 1nm resolution, and with high speed 100Hz spectral acquisition.

MD-4017

Light source: D2 and Wl
Wavelenght range: 200-400 nm
PDA elemens: 512
Noise level: +/- 7,0 x 10-6 Abs
Data Aquisition range: Maximum 20 spectra/s

The MD-4017 uses a CMOS detector covering the UV region from 200-400nm; with 1nm resolution and up to 20Hz spectrum acquisition; useful for peak widths as small as 1 – 2 seconds.

ChromNAV 2.0 PDA Data System

Includes:  multi-functional analysis with spectral analysis, contour map, 3D, library search for peak identification, peak-purity. ChromNAV PDA data processing included as standard.

Fluorscence

Always highest senistivity

The FP-4020 and FP-4025 fluorescence detectors have been designed for the pursuit of extreme sensitivity. A complete redesign of the optics and electronics, as well as removing the effects temperature fluctuation with a new temperature regulated cell body has led to the highest sensitivity with stable detection

Unparalleled Sensitivity

The new axially irradiated cell design enables the very highest sensitivity detection. These changes have resulted in a leap in sensitivity with  signal noise ratio of 2300:1 or higher (water Raman peak). The FP-4025 is an excellent routine fluorescence detector that has a water Raman S/N of 1400:1.

FP-4020 HPLC Fluorescence Detector

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Dual Wavelength Measurement

Both detectors can be used for simultaneous detection at two wavelength pairs; excitation and emission wavelengths can be changed during the acquisition using a time program and automatic spectra scanning can be triggered manually, by time or automatically by threshold. A built-in mercury lamp enables automatic wavelength correction and the front-access design allows easy replacement of the flow cell assembly and light source.

Spectral Scanning

Excitation and emission spectra can be scanned using ChromNAV chromatography data system and can be set to operate, manually, at a specified time or using a threshold trigger.

Control

Individual LC-4000 Series instruments are normally used as part of a complete HPLC system, however for applications (such as adding to a third party system or for a non-LC application) that require stand-alone operation, each instrument has its own keypad and can also be controlled using an RS232 command set, and/or contact closures (TTL) to start and stop the pump.

Refractive Index (RI)

Unmatched sensitivity and stability

The RI-4030/35 refractive index detectors include an optical system mounted in a precise temperature-regulated housing to eliminate the influence of changes in ambient temperature. Both detectors are fully stable just one hour after powering on.

The RI-4030 can be used for analytical and preparative scales. For preparative separations, the maximum flow rate is 120 ml/min.

The RI-4035 is a dedicated semi-micro RI detector with a micro-volume flow cell designed for use with fast semi-micro GPC analysis using columns with a fine pore size.The RI-4035 is a dedicated semi-micro RI detector with a micro-volume flow cell designed for use with fast semi-micro GPC analysis using columns with a fine pore size.

The 100Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC separations. The LED light source in both detectors has a long lifetime (over 10,000 hours) for low maintenance. An optional pressure release safety-valve can be fitted to prevent damage to the flow cell in the event blockage.

RI-4030 Refractive Index HPLC Detector

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Cirular Dichroism Detector

The unike CD-detektor for HPLC

The CD-4095 is a unique HPLC CD detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeters. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column, however the  CD-4095 CD detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring their positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated; a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signals. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.The CD-4095 is a unique HPLC CD detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeters. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column, however the  CD-4095 CD detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring their positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated; a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signals. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.

The CD-4095 can be used with SFC and HPLC.

CD-4095 Circular Dichroism HPLC Detector

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Optical Rotation Detector

Alternative Chiral detector

The OR-4090 chiral detector measures the angle of rotation of plane polarized light caused by optical active isomers and is useful for chiral compounds with no absorption. The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing a strong output in the UV-Visible region where the largest optical rotations offer the highest sensitivity. The wide wavelength range from 350-900nm also allows measurement of a wide variety of samples.The OR-4090 can only be used with HPLC.

Optical Rotation Detector OR-4090

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MS Detector

Small Single Quadropule Detector

The expression CMS range of compact single quadrapole mass spectrometers for small molecule analysis. The CMS can be interfaced with HPLC or SFC for analytical to preparative scale separations. ChromNAV chromatography Data system, includes integrated control for selecting measurement parameters and executing measurement with timed event control for switching polarity and mass range. TIC, EIC and SIM can all be used to monitor chromatograms and to trigger fraction

expression CMS single quadrapole mass spectrometer

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Specificationcms-Ecms-Scms-L
Ion Source   ESI, APCI, or APCI/ASAP (direct analysis probe)
API interface   orthogonal ion sampling from heated capillary – allows for small single turbo pump (Patents pending)
Flow rate range   10 µL/min to 1 mL/min ESI; 10 µL/min to 2 mL/min APCI
Polarity   Positive & Negative ion switching in single analysis
Polarity Switching Speed   50 ms
m/z Range 80 to 800 m/z 10 to 1,200 m/z 10 to 2,000 m/z
Acquisition speed   10,000 m/z units sec-1 (compatible with UHPLC)
Resolution   0.5-0.7 m/z units (FWHM) at 1000 m/z units sec-1 over entire acquisition range
SIM sensitivity   10 pg Reserpine (FIA – 5µL sample injection volume at 100 µL/min) 100:1 S/N (RMS) with SIM of m/z 609.28
Accuracy   ± 0.1 m/z units over entire acquisition range
Dynamic Range   4.5 orders of magnitude

From compact to large for automatic method scouting

Jasco LC-4000 series Column Ovens


From compact to large for automatic method scouting

The LC-4000 Series HPLC column ovens have been designed to provide excellent utility for a wide range of different user requirements.

All HPLC column ovens have built-in electronic cooling for controlling column temperature at sub-ambient temperatures. The CO-4062 and CO-4061 block-heater type ovens can be fitted with an optional micro-volume pre-heating unit for pre- heating the mobile phase before the column to improve resolution and peak shape.

HPLC CO-4061/62 Compact

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Compact and stackable ovens

CO-4061
For simple requirements the stackable CO-4061 can be used with column lengths up to 200mm (or up to 300mm with an optional extension).

CO-4062
For RHPLC and UHPLC the CO-4062 has capacity for up to six 150 mm length columns, with optional one or two internally mounted automatic switching valves for 2 or 6 column selection.

CO-4061/4062

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For preparative LC or GPC

For GPC and larger preparative columns, select either of the two high-capacity CO-4060 or CO-4065 HPLC column ovens.

CO-4060
The CO-4060 can accommodate columns with lengths up to 300mm or larger ID preparative columns.

CO-4065
The CO-4065 can accommodate up to 10 columns with lengths up to 300mm and a pair of column selection valves is included as standard.

CO-4060/4065

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RO-4068 Reaction Oven

The RO-2068 reaction oven is used for high temperature post-column sample processing with capacity for columns and reaction coils. with lengths up to 300mm or larger ID preparative columns. The CO-4065 can accommodate up to 10 columns with lengths up to 300mm and a pair of column selection valves is included as standard.

RO-4068

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Pre-Column Temperature Stabilizer

Analysis Conditions

Column X-Presspak VC18-W
(3 mmI.D. x 50 mmL, 2 μm)
Eluent Water / Acetonitrile (Gradient)
Flow Rate 1.0mL/min
Sample Gasoline Oxidization Products

Comparison of Toluene Peaks

Room Temp.Oven Temp.Pre-Column StabilizerTheoreticalPeak WidthtR(min)
21°C 25°C With 56095 0.019 1.893
Without 54747 0.019 1.897
40°C With 46021 0.020 1.799
Without 36402 0.023 1.851

ChromNAV - The “PowerTool” for HPLC Analysis

ChromNAV Chromatography Software


The “PowerTool” for HPLC Analysis

ChromNAV 2.0 HPLC software is the latest Chromatography Data System (CDS) with a host of exciting new features. Using the customizable graphical-user-interface (GUI), the system can be quickly set up to display only the functions necessary for your application. The user can easily learn the operation and explore the extensive functionality for control and data processing.

ChromNAV 2.0

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Versatile

ChromNAV 2.0 is a universal CDS which can be used with any type of analytical separation, including: HPLC, UHPLC, SFC and preparative HPLC & SFC. ChromNAV MS also includes integrated control of a single quadrapole MS detector for both LC and SFC

Powerful System Control for Method Development

ChromNAV 2.0 offers flexible system control and data acquisition with options for 2D (or up to 4D) gradient control and multi-zone oven control (up to 3) for method development. Solvent and column selection has been increased to include up to 10 positions for both. During acquisition, the run-time for a chromatogram can be increased to capture later eluting peaks that may exceed the set run-time.

Data Rich

During acquisition a ‘known’ chromatogram can be overlaid for visual comparison with the currently separation, as a quick indicator to the user of the reliability current separation.The fluorescence and UV-visible detectors both include the spectral scanning, which can be triggered manually, by time or by threshold. Data acquisition and analysis for PDA detectors are included as standard in ChromNAV. A newly added feature is the ability to send an automated email to your PC or smartphone with an update on the progress of the sequence.

Green

As part of JASCO’s commitment to Green Energy, the LC-4000 Series includes both ‘power off’ for the flow, lamp, column oven etc at the end of the sequence, and a ‘soft-off’ function to automatically completely shut down selected modules to save energy.

Control Method

The pump flow rate and gradient profile display is flexible and can be overlaid with a chromatogram for adjusting gradient conditions.The pump flow rate and gradient profile display is flexible and can be overlaid with a chromatogram for adjusting gradient conditions.

PDA Analysis

PDA data processing is included as standard. Data is displayed in 2-D and 3-D contour plot with simultaneous overlay of spectra and chromatograms. Extract chromatograms at single or multiple wavelengths for quantitation.

ChromNav 2.0 Standard Features

  • Peak integration and identification
  • Peak grouping
  • Linear and non-linear quantitation
  • 3D chromatogram analysis
  • Spectral analysis for UV-visible, Fluorescence and PDA detectors
  • Style report generator
  • User calculation


All raw data is protected and saved, which can then be analyzed and re-analyzed, reported and saved with both the raw data and any data processing from the user’s analysis

.A comprehensive audit trail records the acquisition method along with a history of instrument performance indicators in each sample data file, which provides the user a snap-shot of the condition of the system during the run and can warn about requirements for impending maintenance.

ChromNAV 2.0 Optional Application Modules

  • ChromNAV CFR for 21 CFR Part 11 compliance and electronic registration of data.
  • ChromNAV GPC for estimating molecular weight dispersion from retention time.
  • ChromNAV FC for fraction collection control (can be used for both LC and SFC fractionation).

  • ChromNAV MS control of single quadrapole MS detector with ESI or APCI sources.
  • Molecular weight dispersion of low molecular weight heparin FUMI theory (Function of Mutual Information) for theoretical precision analysis.

ChromNAV GPC

Molecular weight distribution program ChromNAV GPC/SEC calculation program for GPC analysis

ChromNAV Fraction Collection view

ChromNAV-FC fraction collector control for the CHF-122SC fraction collector (option)

ChromNAV-FC for Preparative HPLC

The optional ChromNAV-FC fraction collector control is available as part of the Prep-HPLC kit and provides ChromNAV with the capability to control the LC-4000 Series preparative HPLC system for fraction collection.The optional ChromNAV-FC fraction collector control is available as part of the Prep-HPLC kit and provides ChromNAV with the capability to control the LC-4000 Series preparative HPLC system for fraction collection.

ChromNAV-FC offers control for two different capacity fraction collectors, with advanced fractionation algorithms for peak collection, using time, threshold and slope, and can even be programmed to use data collected simultaneously from multiple detectors such as UV-visible, refractive index or circular dichroism. Fraction simulation uses an acquired chromatogram to develop the fraction collection parameters and the graphical display can be used to locate peaks of interest in the fraction collector tray.

ChromNAV-FC also supports additional functions for prep-HPLC applications, including automatic multi-channel fractions, fraction repeat and fraction re-cycle.

KOVALENTS PHILOSOPHY

At Kovalent we strive to establish and develop long-term partnerships with our customers. When we use the word “partnership” we mean that Kovalent becomes an integral part of our customers’ projects, where we are expected to fully understand their application needs. We aim to make Kovalent our customers’ first and natural choice, and this ambition permeates our entire organisation from initial contact to finished and delivered product.

CONTACTS:

Kovalent AB
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SKANDINAVISKA GENETEC AB

Genetec is a member of the same family of companies as Kovalent. Both companies share the same set of values and are in many ways integrated with each other. The main difference between the two companies are their products. Kovalent is involved in system solutions including instrumentation and Genetec is mainly working with consumables. The focus of Genetec is chromatography accessories. You are welcome to visit their state of the art web shop: genetec.se