Jasco LC-4000 series Detectors
UV-visible
Pioneering Optical Design
The UV-4070 (190 – 900nm) and UV-4075 (190 – 700nm) UV-visible HPLC detectors both use an advanced Czerny-Turner monochromator with dual wavelength and scanning functions. Baseline change due to refractive index is minimized by the flow cell design, and fluctuation from changes in environmental and lamp temperature are reduced with a regulated lamp housing and temperature-controlled cell.
Optional flow cells
Semi-micro, semi-micro SP (UHPLC), preparative and high pressure for SFC
UV-4070/4075 detectors
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Spectral Scanning and Dual Wavelength Detection
Both detectors include dual-wavelength data acquisition (up to 100Hz for UHPLC) and spectral scanning. Spectral scanning can be executed automatically in ChromNAV CDS, by time or by height when a peak exceeds a set threshold.
Easy User Maintenance
User maintenance can be done without removing the module from the system. Light source replacement is made through the front panel and automatic wavelength calibration can be carried out using a mercury lamp
Operation as a Stand-Alone Instrument
The UV-4000 series detectors have a keypad for direct control, parameters can be easily changed and operated in a time program for control throughout a separation (this can be be triggered using a TTL pulse or contact closure). A simple RS232 protocol can be used for control through third part programs.
PDA
High Sensitivity and Flexibility
The LC-4000 Series includes three PDA detectors. The MD-4017 is a simple replacement for a single wavelength detector, the MD-4015 is the workhorse for UV-Visible detection in the range 200 to 600nm and the MD-4010 has a wide wavelength range.
Optional flow cells
Semi-micro, semi-micro SP (UHPLC), preparative and high pressure for SFC
MD-4010/4015/4017
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MD-4010
Light source: D2 and Wl
Wavelenght range: 190-900 nm
PDA elemens: 1024
Noise level: +/- 3,0 x 10-6 Abs
Data Aquisition range: Maximum 100 spectra/s
The MD-4010 PDA detector has high sensitivity, comparable to a single wavelength detector. It uses a 1024 element photometric diode array, with measurement wavelength range from 190nm to 900nm and resolution of 1nm. Fast spectral acquisition rate of up to 100 spectra/sec. The temperature controlled SP type semi-micro flow cell is used narrow peaks typically found with fast UHPLC or RHPLC separations. An integrated mercury lamp is used for automatic wavelength calibration and validation.
MD-4015
Light source: D2
Wavelenght range: 200-600 nm
PDA elemens: 512
Noise level: +/- 3,0 x 10-6 Abs
Data Aquisition range: Maximum 100 spectra/s
The MD-4015 PDA detector is similar to the MD-4010 with equivalent sensitivity, covering the wavelength range from 200nm to 650nm using a 512 element PDA for 1nm resolution, and with high speed 100Hz spectral acquisition.
MD-4017
Light source: D2 and Wl
Wavelenght range: 200-400 nm
PDA elemens: 512
Noise level: +/- 7,0 x 10-6 Abs
Data Aquisition range: Maximum 20 spectra/s
The MD-4017 uses a CMOS detector covering the UV region from 200-400nm; with 1nm resolution and up to 20Hz spectrum acquisition; useful for peak widths as small as 1 – 2 seconds.
ChromNAV 2.0 PDA Data System
Includes: multi-functional analysis with spectral analysis, contour map, 3D, library search for peak identification, peak-purity. ChromNAV PDA data processing included as standard.
Fluorscence
Always highest senistivity
The FP-4020 and FP-4025 fluorescence detectors have been designed for the pursuit of extreme sensitivity. A complete redesign of the optics and electronics, as well as removing the effects temperature fluctuation with a new temperature regulated cell body has led to the highest sensitivity with stable detection
Unparalleled Sensitivity
The new axially irradiated cell design enables the very highest sensitivity detection. These changes have resulted in a leap in sensitivity with signal noise ratio of 2300:1 or higher (water Raman peak). The FP-4025 is an excellent routine fluorescence detector that has a water Raman S/N of 1400:1.
FP-4020 HPLC Fluorescence Detector
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Dual Wavelength Measurement
Both detectors can be used for simultaneous detection at two wavelength pairs; excitation and emission wavelengths can be changed during the acquisition using a time program and automatic spectra scanning can be triggered manually, by time or automatically by threshold. A built-in mercury lamp enables automatic wavelength correction and the front-access design allows easy replacement of the flow cell assembly and light source.
Spectral Scanning
Excitation and emission spectra can be scanned using ChromNAV chromatography data system and can be set to operate, manually, at a specified time or using a threshold trigger.
Control
Individual LC-4000 Series instruments are normally used as part of a complete HPLC system, however for applications (such as adding to a third party system or for a non-LC application) that require stand-alone operation, each instrument has its own keypad and can also be controlled using an RS232 command set, and/or contact closures (TTL) to start and stop the pump.
Refractive Index (RI)
Unmatched sensitivity and stability
The RI-4030/35 refractive index detectors include an optical system mounted in a precise temperature-regulated housing to eliminate the influence of changes in ambient temperature. Both detectors are fully stable just one hour after powering on.
The RI-4030 can be used for analytical and preparative scales. For preparative separations, the maximum flow rate is 120 ml/min.
The RI-4035 is a dedicated semi-micro RI detector with a micro-volume flow cell designed for use with fast semi-micro GPC analysis using columns with a fine pore size.The RI-4035 is a dedicated semi-micro RI detector with a micro-volume flow cell designed for use with fast semi-micro GPC analysis using columns with a fine pore size.
The 100Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC separations. The LED light source in both detectors has a long lifetime (over 10,000 hours) for low maintenance. An optional pressure release safety-valve can be fitted to prevent damage to the flow cell in the event blockage.
RI-4030 Refractive Index HPLC Detector
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Cirular Dichroism Detector
The unike CD-detektor for HPLC
The CD-4095 is a unique HPLC CD detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeters. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column, however the CD-4095 CD detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring their positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated; a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signals. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.The CD-4095 is a unique HPLC CD detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeters. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column, however the CD-4095 CD detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring their positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated; a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signals. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.
The CD-4095 can be used with SFC and HPLC.
CD-4095 Circular Dichroism HPLC Detector
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Optical Rotation Detector
Alternative Chiral detector
The OR-4090 chiral detector measures the angle of rotation of plane polarized light caused by optical active isomers and is useful for chiral compounds with no absorption. The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing a strong output in the UV-Visible region where the largest optical rotations offer the highest sensitivity. The wide wavelength range from 350-900nm also allows measurement of a wide variety of samples.The OR-4090 can only be used with HPLC.
Optical Rotation Detector OR-4090
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MS Detector
Small Single Quadropule Detector
The expression CMS range of compact single quadrapole mass spectrometers for small molecule analysis. The CMS can be interfaced with HPLC or SFC for analytical to preparative scale separations. ChromNAV chromatography Data system, includes integrated control for selecting measurement parameters and executing measurement with timed event control for switching polarity and mass range. TIC, EIC and SIM can all be used to monitor chromatograms and to trigger fraction
expression CMS single quadrapole mass spectrometer
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| Specification | cms-E | cms-S | cms-L |
|---|
| Ion Source |
|
ESI, APCI, or APCI/ASAP (direct analysis probe) |
| API interface |
|
orthogonal ion sampling from heated capillary – allows for small single turbo pump (Patents pending) |
| Flow rate range |
|
10 µL/min to 1 mL/min ESI; 10 µL/min to 2 mL/min APCI |
| Polarity |
|
Positive & Negative ion switching in single analysis |
| Polarity Switching Speed |
|
50 ms |
| m/z Range |
80 to 800 m/z |
10 to 1,200 m/z |
10 to 2,000 m/z |
| Acquisition speed |
|
10,000 m/z units sec-1 (compatible with UHPLC) |
| Resolution |
|
0.5-0.7 m/z units (FWHM) at 1000 m/z units sec-1 over entire acquisition range |
| SIM sensitivity |
|
10 pg Reserpine (FIA – 5µL sample injection volume at 100 µL/min) 100:1 S/N (RMS) with SIM of m/z 609.28 |
| Accuracy |
|
± 0.1 m/z units over entire acquisition range |
| Dynamic Range |
|
4.5 orders of magnitude |
