Jasco LC-4000 series HPLC Pumps
Robust pump solutions from UHPLC to preparative clean-up in gram scale
The LC-4000 Series HPLC pumps have 3 operating pressures RHPLC – rapid (700bar), UHPLC – ultra high performance (1300 bar) and preparative (500 bar). Each base has two expansion bays that allow an isocratic pump to be transformed into a powerful gradient solvent delivery system with optional solvent selection for method development.
Jasco LC-4000 series HPLC Pumps
HPLC, RHPLC PU-4180, Analytical Pump
RHPLC Pumps
Options: Isocratic, Binary (two pumps and high pressure mixer) and Quaternary (four solvent gradient mixer with degasser)
Flow Rate: Up to 5 mL/min gradient (10mL/min isocratic)
Operating Pressure: Up to 700 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
Flow Rate: Up to 5 mL/min gradient (10mL/min isocratic)
Operating Pressure: Up to 700 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
RHPLC, PU-4185 Semi-Micro Pump
RHPLC Semi- Micro Pumps
Options: Isocratic, (two pumps and high pressure mixer)
Flow Rate: Up to 4 mL/min
Operating Pressure: Up to 700 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
Flow Rate: Up to 4 mL/min
Operating Pressure: Up to 700 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
UHPLC, PU-4285 Semi-Micro Pump
UHPLC Pumps
Options: Isocratic, Binary (two pumps and high pressure mixer)
Flow Rate: Up to 2 mL/min gradient (10mL/min isocratic)
Operating Pressure: Up to 1,300 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
Flow Rate: Up to 2 mL/min gradient (10mL/min isocratic)
Operating Pressure: Up to 1,300 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
PREPARATIVE, PU-4086/87/88 preparative pumps
Preparative Pumps
Options: Isocratic, (two pumps and high pressure mixer)
Flow Rate: Up to 20 mL/min (PU-4086)
Flow Rate: Up to 50 mL/min (PU-4087)
Flow Rate: Up to 150 mL/min (PU-4088)
Operating Pressure: Up to 500 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
Flow Rate: Up to 20 mL/min (PU-4086)
Flow Rate: Up to 50 mL/min (PU-4087)
Flow Rate: Up to 150 mL/min (PU-4088)
Operating Pressure: Up to 500 Bar
Degassing: Vacuum (Option)
Solvents: 2, 6 or 10
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Configuration
The unique expansion bays in the pump base can be fitted with plug-in modules to quickly transform a simple isocratic pumping system into a multi-solvent delivery system. Install a second pump (with dynamic mixer) for binary gradients or a quaternary low pressure mixing gradient unit (four solvent), either can be configured with an optional low volume vacuum degasser. A 6 – or 10 solvent selection valve can be added for method development. -
Control
The PU-4000 Series pumps are normally used as part of a complete HPLC system, however for applications that require stand-alone operation, each instrument has its own keypad and can be controlled using an RS232 protocol and/or contact closures (TTL) to start and stop the pump.
Retention Time Reproducibility
Isocratic Retention time Reproducibility – PU-4180
Isocratic retention time reproducibility and flow accuracy- PU-4180 /1 ml/min)
Conditions
| Mobile Phase | Acetonitrile/Water (80:20) |
| Flow Rate | 1.0mL/min |
| Column | CrestPak C18S (4.6mml.D. x 150mm, 5µm) |
| Column Temperature | 40° C |
| Detection Wave Length | 260nm, Response; 1sec |
| Sample | Polycyclic aromatic hydrocarbon (10µg/mL each) (Naphthalene, Fluorene, Anthracene, Pyrene, Chrysene, Benzon[a]pyrene) |
| Injection | 10 µL |