Jasco SFE - Supercritical Fluid Extraction

Environmentally clean extraction technique

Supercritical fluid extraction (SFE) uses a supercritical fluid as an extraction solvent. Selective extraction can be made by exploiting the properties of differential solubility of target analytes in a wide variety of matrices.

Brochure

Jasco SFE

Supercritical Fluid Extraction employs a supercritical fluid, most commonly CO2, as the A mobile phase solvent for the extraction. The intrinsic low viscosity and high diffusivity of supercritical CO2 has rendered SFE a faster extraction and higher efficiency technique when compared to traditional liquid extraction. This provides faster flow rates and thus faster extraction times without the requirement for a higher pressure system. The addition of a co-solvent to the CO2 flow can help tune the strength further. The extraction oven can offer temperatures up to 100°C. Lastly after the extraction vessel is the back pressure regulator, which provides the back pressure requirement to keep the CO2 supercritical, and is an integral part of the performance of the extraction.

SFE Benefits

1. Selectivity

CO2 polarity varies greatly depending upon the pressure it is exposed to. This makes CO2 a tunable solvent which allows the user to find the precise conditions for extraction of the compounds of interest while leaving unwanted compounds behind. This inherent trait of CO2 greatly reduces the need for post extraction cleanup that would be necessary with most solvent extractions.
2. No residual solvents

Given the gaseous state of CO2 at atmospheric conditions, the resulting extract does not require the long rotovap time needed to dry solvent extracted analytes.
3. Faster

Given the high diffusivity and low viscosity of CO2 in its supercritical state the extractions typically take a fraction of the time compared to solvent extractions.
4. Higher Yield

Due to increased temperature and pressure, supercritical CO2 can penetrate many matrices that solvents cannot thereby allowing for greater surface area contact which in turn increases yield.
5. Low Operating Cost

Cost per extraction is significantly lower as the cost of CO2 is much lower than the equivalent amount of solvent.
6. Environmentally Friendly

Cost per extraction is significantly lower as the cost of CO2 is much lower than the equivalent amount of solvent.
When compared to Soxhlet extractions, supercritical fluid extractions have proven to be capable of providing extractions up to 25 times faster with equivalent recovery while using up to 30 times less solvent.

Product Specialist

Mats Grapne

"More than 30 years experience in the field of HPLC, Spectroscopy and other analytical equipments. Contact me if you have questions or need a quotation"

mats.grapne@kovalent.se

08-473 33 40

Analytical SFE

● The analytical CO2 pump offers built-in peltier cooling to maintain a stable CO2 flow yielding excellent extraction time reproducibility. Automatic, built-in shut-off valves close the CO2 inlet and outlet and isolate the pumps for quick and simple priming when not pumping.
● The system can be configured for CO2 only or 1-10 co-solvents, 1-10 extraction vessels for the vessel volumes listed above and 1, 6, 12 or 54 fractions.

System Extractions vessel CO2 Flowrate

Analytical 1 ml, 5 ml, 10 ml 0,2-10 ml

Hybrid SFE

● The hybrid CO2 pump offers a flow range from 0.5 to 20 mL/min covering both analytical 10mL vessels up to semi-prep 100 mL vessels. Automatic, built-in shut-off valves close the CO2 inlet and outlet and isolate the pumps for quick and simple priming when not pumping.
● The system can be configured for CO2 only or 1-10 co-solvents, 1-10 extraction vessels for the vessel volumes listed above and 1, 6, 12 or 54 fractions.

System Extractions vessel CO2 Flowrate

Hybrid 10 ml, 50 ml, 100 ml 0,5-20 ml

Semi-Prep SFE

● The semi-prep CO2 pump offers a flow range from 3 to 50 mL/min covering 50 mL vessels up to 200 mL vessels (largest vessels not availible yet). Automatic, built-in shut-off valves close the CO2 inlet and outlet and isolate the pumps for quick and simple priming when not pumping.
● The system can be configured for CO2 only or 1-10 co-solvents, 1-10 extraction vessels for the vessel volumes listed above and 1, 6, 12 or 54 fractions.

System Extractions vessel CO2 Flowrate

Semi-Prep 50 ml, 100 ml, (200 ml) 3-50 ml

Preparative SFE

● The Prep CO2 pump offers a flow range from 5 to 150 mL/min covering 500 mL vessels up to 2L vessels (largest vessels not availible yet). Automatic, built-in shut-off valves close the CO2 inlet and outlet and isolate the pumps for quick and simple priming when not pumping.
● The system can be configured for CO2 only or 1-10 co-solvents, 1-10 extraction vessels for the vessel volumes listed above and 1, 6, 12 or 54 fractions.

System Extractions vessel CO2 Flowrate

Prep (500 ml, 1 L, 2 L) 5-150 ml