Fully automated sample analysis and evaluation system for routine quality control

Camag HPTLC Pro - NEW!


Fully automated sample analysis and evaluation system for routine quality control

System description

Fully automated sample analysis and evaluation system using HPTLC plates (20 x 10 cm). The HPTLC PRO SYSTEM (patented technology) is best suited for routine quality control of analytes extracted from complex matrices, providing reproducible and reliable results. It supports up to 75 samples, up to five plates, and up to three independent developing solvents without intervention. The possibility of derivatization, MS coupling, and plate storage are included.

  • ● Application- The HPTLC PRO Module DEVELOPMENT

  • ● Derivatization - The HPTLC PRO Module DERIVATIZATION

  • ● Detection - The HPTLC PRO Module DETECTION -expected 2021

  • ● MS-Interface - The HPTLC PRO Module MS-INTERFACE -expected 2020

  • ● Plate Storage - The HPTLC PRO Module PLATE STORAGE -expected 2020

  • ● HPTLC Software visionCATS - Automates the entire automatic process

Product Specialist

Dag Dacke

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
070-7319009
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Products

Application

The samples are applied as bands onto the HPTLC glass plate (20 x 10 cm) with the HPTLC PRO Module APPLICATION. Up to 75 samples can be applied autonomously. Precision of the applied volume, exact positioning and sharpness of applied zones determine the quality of the final result.

Development

The HPTLC PRO Module DEVELOPMENT, using HPTLC glass plates (20 x 10 cm), operates completely autonomously. The activity of the stationary phase can be adjusted, and the gas phase can be actively circulated during development (conditioning, regeneration), allowing a better separation of the analytes.

Derivatization

Substances without chromophores or fluorophores can be visualized by automated spraying of specific reagents onto the HPTLC glass plates (20 x 10 cm) with the HPTLC PRO Module DERIVATIZATION, controlled by the HPTLC Software visionCATS. The module features an integrated plate heating unit.

Detection

Hyperspectral data, acquired with the HPTLC PRO Module DETECTION, are used to characterize and quantify all analytes. They are obtained by using polychromatic light or specific LEDs for excitation together with an imaging spectrometer. The HPTLC Software visionCATS is used for data evaluation.

MS-Interface

HPTLC-MS coupling allows for verification of the chemical structure of analytes by Mass Spectrometry or offline collection for further analysis. Any position on the plate can be selected via the HPTLC Software visionCATS to be automatically eluted with the HPTLC PRO Module MS-INTERFACE.

Plate Storage

This Module is required to run multiple plates with the HPTLC PRO SYSTEM autonomously. It consists of two closed compartments, one holding up to five new HPTLC glass plates and one up to five finished plates.

Software Integration

CAMAG’s HPTLC Software visionCATS organizes the workflow of the HPTLC PRO analysis, controls the HPTLC PRO Modules, and manages all data evaluation.

HPTLC instruments for qualitative and quantitative analyses

Camag HPTLC


HPTLC instruments for qualitative and quantitative analyses

It comprises the use of chromatographic layers of utmost separation efficiency and the employment of state-of-the-art instrumentation for all steps in the procedure: precise sample application, standardized reproducible chromatogram development and software controlled evaluation

Brochure

System description

HPTLC is an entire concept that includes a widely standardized methodology based on scientific facts as well as the use of validated methods for qualitative and quantitative analysis. HPTLC meets all quality requirements of today’s analytical labs, even in a fully regulated environment.

As opposed to other separation techniques, the inital costs for an HPTLC system as well as maintenance, and costs per sample are comparatively low. The possibility of visual evaluation of separated samples on the plate is one of the most valuable aspects of TLC. It reaches a completely new dimension in HPTLC through the use of state-of-the-art techniques for generating and evaluating digital images.

  • ● No sample preparation! Just apply your sample(s) on the plate
  • ● Run many samples in parallell
  • ● View and compare several samples or standards towards eachother in real time
  • ● Revover sample or compunds directly from plate
  • ● Attach MS-interface for direct transfer of compounds into LCMS-system
  • ● Compare separation pattern to databases for Herbs and other species. 
Product Specialist

Dag Dacke

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me for if you have questions or need a quotation"

070-7319009

STEPS OF THE TLC/HPTLC PROCEDURE

Sample Application

The samples are applied onto the plate either as spots or as bands. Precision of the applied volume, exact positioning and compactness of application zone determine the quality of the final result. It can be done manuallyor semi-automatet with with Nanomat 4 or Linomat 5. Fully automatic with ATS 4 autosampler.

Chromatogram Development

The mobile phase is drawn through the stationary phase by capillary action. Samples are separated into their components which remain in their position on the layer after the mobile phase has been evaporated. Chromatography is affected by the gas phase in the chromatographic chamber.

Derivatization

Substances without chromophores or color can be visualized or made detectable through derivatization. The required reagents are transferred onto the chromatogram by spaying or immersion.

Chromatogram Evaluation

The chromatogram is evaluated under white or ultraviolet light. Options range from visual inspection of electronic images to quantitative determinations using scanning densitometry. For documentation electronic images are easy to capture and to archive. They can be reproduced on screen without changes over time and thus compared with current images. TLC-MS and bioluminescence expand the capability of TLC.

Visualizer

For electronic image acquisition the camera – like the human eye – captures polychromatic visible light. Under white light illumination it is the light reflected from the layer background. Under long-wavelength UV light (366 nm) it is the light emitted by fluorescent substances. When short-wavelength UV light (254 nm) is used, substances absorbing UV 254 nm appear as dark zones, provided the layer contains a fluorescence indicator (fluorescence quenching). The TLC Visualizer 2 is CAMAG’s imaging and documentation system operated with visionCATS. visionCATS enables an image-based evaluation of chromatograms for quantitative evaluation. The strength of the electronic image acquisition is the overview of the complete chromatogram.

TLC Scanner 4

 The spectral range of the CAMAG TLC Scanner 4 is 190–900 nm. Reflected light is measured either in the absorbance or in the fluorescence mode. From the acquired data quantitative results are computed with high precision and spectral selectivity.
With the TLC Scanner 4 absorption spectra can be recorded. The strengths of classical densitometry as compared with image evaluation are spectral selectivity and the higher precision of quantitative determinations.

TLC- MS-Interface

Through the pioneering concept of hyphenating High-Performance Thin-Layer Chromatography with Mass Spectrometry unequivocal substance identification is possible. The CAMAG TLC-MS Interface 2 can be installed plug & play with any LC-MS system without adjustments or mass spectrometer modifications. Depending on the MS system, a substance can be identified within a minute via its mass spectrum, or for an unknown substance zone, the respective sum formula can be obtained. Furthermore, interesting zones can be eluted into vials for further investigations with, e.g., NMR, (ATR-)FTIR, ESI-MS, and MALDI-MS.

TLC

Devices for conventional TLC

Camag TLC


Thin-Layer Chromatography (TLC) is a simple, flexible and cost efficient separation technique for both qualitative and quantitative analysis

A separation technique for both qualitative and quantitative analysis, enabling simultaneous analysis of many substances with minimal time requirement. TLC can be performed manually in easy and inexpensive ways. Therefore it is found in almost all laboratories as a convenient tool for simple and rapid separations. As the expectations grow concerning quality and value of an analysis, there are suitable instruments available for all steps of TLC.

Brochure

System description

Thin-Layer Chromatography (TLC) is a well-known technique in laboratories for ages. It can be performed in a very easy way without the need for advanced equipment. Samples can be attached by hand to the TLC-plate. Separation is performed in a development chamber by using a mobile phase. Plate is dryed and evaluated by human eye or in a chamber under UV-light. Sometmes a derivatisation step is needed in-between.
  • ● No sample preparation! Just apply your sample(s) on the plate
  • ● Run many samples in parallell
  • ● View and compare several samples or standards towards eachother in real time
  • ● Revover sample or compunds directly from plate
  • ● Attach MS-interface for direct transfer of compounds into LCMS-system
  • ● Compare separation pattern to databases for Herbs and other species. 

Product Specialist

Dag Dacke

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me for if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
070-7319009

STEPS OF THE TLC PROCEDURE

Sample Application

The samples are applied onto the plate either as spots or as bands. Precision of the applied volume, exact positioning and compactness of application zone determine the quality of the final result. It can be done manuallyor semi-automatet with with Nanomat 4 or Linomat 5.

Chromatogram Development

The mobile phase is drawn through the stationary phase by capillary action. Samples are separated into their components which remain in their position on the layer after the mobile phase has been evaporated. Chromatography is affected by the gas phase in the chromatographic chamber.

Derivatization

Substances without chromophores or color can be visualized or made detectable through derivatization. The required reagents are transferred onto the chromatogram by spaying or immersion.

Chromatogram Evaluation

The chromatogram is evaluated under white or ultraviolet light. 

KOVALENTS PHILOSOPHY

At Kovalent we strive to establish and develop long-term partnerships with our customers. When we use the word “partnership” we mean that Kovalent becomes an integral part of our customers’ projects, where we are expected to fully understand their application needs. We aim to make Kovalent our customers’ first and natural choice, and this ambition permeates our entire organisation from initial contact to finished and delivered product.

CONTACTS:

Kovalent AB
Hulda Lindgrens gata 6A
421 31 VÄSTRA FRÖLUNDA
Sweden
Phone: +46(0)31-709 81 80
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Org.nr: 556417-2194

SKANDINAVISKA GENETEC AB

Genetec is a member of the same family of companies as Kovalent. Both companies share the same set of values and are in many ways integrated with each other. The main difference between the two companies are their products. Kovalent is involved in system solutions including instrumentation and Genetec is mainly working with consumables. The focus of Genetec is chromatography accessories. You are welcome to visit their state of the art web shop: genetec.se