Fully automated sample analysis and evaluation system using HPTLC plates (20 x 10 cm). The HPTLC PRO SYSTEM (patented technology) is best suited for routine quality control of analytes extracted from complex matrices, providing reproducible and reliable results. It supports up to 75 samples, up to five plates, and up to three independent developing solvents without intervention. The possibility of derivatization, MS coupling, and plate storage are included.

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me if you have questions or need a quotation"

The samples are applied as bands onto the HPTLC glass plate (20 x 10 cm) with the HPTLC PRO Module APPLICATION. Up to 75 samples can be applied autonomously. Precision of the applied volume, exact positioning and sharpness of applied zones determine the quality of the final result.

The HPTLC PRO Module DEVELOPMENT, using HPTLC glass plates (20 x 10 cm), operates completely autonomously. The activity of the stationary phase can be adjusted, and the gas phase can be actively circulated during development (conditioning, regeneration), allowing a better separation of the analytes.

Substances without chromophores or fluorophores can be visualized by automated spraying of specific reagents onto the HPTLC glass plates (20 x 10 cm) with the HPTLC PRO Module DERIVATIZATION, controlled by the HPTLC Software visionCATS. The module features an integrated plate heating unit.

Hyperspectral data, acquired with the HPTLC PRO Module DETECTION, are used to characterize and quantify all analytes. They are obtained by using polychromatic light or specific LEDs for excitation together with an imaging spectrometer. The HPTLC Software visionCATS is used for data evaluation.

HPTLC-MS coupling allows for verification of the chemical structure of analytes by Mass Spectrometry or offline collection for further analysis. Any position on the plate can be selected via the HPTLC Software visionCATS to be automatically eluted with the HPTLC PRO Module MS-INTERFACE.

This Module is required to run multiple plates with the HPTLC PRO SYSTEM autonomously. It consists of two closed compartments, one holding up to five new HPTLC glass plates and one up to five finished plates.

CAMAG’s HPTLC Software visionCATS organizes the workflow of the HPTLC PRO analysis, controls the HPTLC PRO Modules, and manages all data evaluation.

It comprises the use of chromatographic layers of utmost separation efficiency and the employment of state-of-the-art instrumentation for all steps in the procedure: precise sample application, standardized reproducible chromatogram development and software controlled evaluation

HPTLC is an entire concept that includes a widely standardized methodology based on scientific facts as well as the use of validated methods for qualitative and quantitative analysis. HPTLC meets all quality requirements of today’s analytical labs, even in a fully regulated environment.

As opposed to other separation techniques, the inital costs for an HPTLC system as well as maintenance, and costs per sample are comparatively low. The possibility of visual evaluation of separated samples on the plate is one of the most valuable aspects of TLC. It reaches a completely new dimension in HPTLC through the use of state-of-the-art techniques for generating and evaluating digital images.

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me for if you have questions or need a quotation"

STEPS OF THE TLC/HPTLC PROCEDURE

Sample Application

Chromatogram Development

Derivatization

Chromatogram Evaluation

Visualizer

For electronic image acquisition the camera – like the human eye – captures polychromatic visible light. Under white light illumination it is the light reflected from the layer background. Under long-wavelength UV light (366 nm) it is the light emitted by fluorescent substances. When short-wavelength UV light (254 nm) is used, substances absorbing UV 254 nm appear as dark zones, provided the layer contains a fluorescence indicator (fluorescence quenching). The TLC Visualizer 2 is CAMAG’s imaging and documentation system operated with visionCATS. visionCATS enables an image-based evaluation of chromatograms for quantitative evaluation. The strength of the electronic image acquisition is the overview of the complete chromatogram.

TLC Scanner 4

 The spectral range of the CAMAG TLC Scanner 4 is 190–900 nm. Reflected light is measured either in the absorbance or in the fluorescence mode. From the acquired data quantitative results are computed with high precision and spectral selectivity.
With the TLC Scanner 4 absorption spectra can be recorded. The strengths of classical densitometry as compared with image evaluation are spectral selectivity and the higher precision of quantitative determinations.

TLC- MS-Interface

Through the pioneering concept of hyphenating High-Performance Thin-Layer Chromatography with Mass Spectrometry unequivocal substance identification is possible. The CAMAG TLC-MS Interface 2 can be installed plug & play with any LC-MS system without adjustments or mass spectrometer modifications. Depending on the MS system, a substance can be identified within a minute via its mass spectrum, or for an unknown substance zone, the respective sum formula can be obtained. Furthermore, interesting zones can be eluted into vials for further investigations with, e.g., NMR, (ATR-)FTIR, ESI-MS, and MALDI-MS.

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me for if you have questions or need a quotation"

The samples are applied onto the plate either as spots or as bands. Precision of the applied volume, exact positioning and compactness of application zone determine the quality of the final result. It can be done manuallyor semi-automatet with with Nanomat 4 or Linomat 5.

The chromatogram is evaluated under white or ultraviolet light.