It comprises the use of chromatographic layers of utmost separation efficiency and the employment of state-of-the-art instrumentation for all steps in the procedure: precise sample application, standardized reproducible chromatogram development and software controlled evaluation
HPTLC is an entire concept that includes a widely standardized methodology based on scientific facts as well as the use of validated methods for qualitative and quantitative analysis. HPTLC meets all quality requirements of today’s analytical labs, even in a fully regulated environment.
As opposed to other separation techniques, the inital costs for an HPTLC system as well as maintenance, and costs per sample are comparatively low. The possibility of visual evaluation of separated samples on the plate is one of the most valuable aspects of TLC. It reaches a completely new dimension in HPTLC through the use of state-of-the-art techniques for generating and evaluating digital images.
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The samples are applied onto the plate either as spots or as bands. Precision of the applied volume, exact positioning and compactness of application zone determine the quality of the final result. It can be done manuallyor semi-automatet with with Nanomat 4 or Linomat 5. Fully automatic with ATS 4 autosampler.
For electronic image acquisition the camera – like the human eye – captures polychromatic visible light. Under white light illumination it is the light reflected from the layer background. Under long-wavelength UV light (366 nm) it is the light emitted by fluorescent substances. When short-wavelength UV light (254 nm) is used, substances absorbing UV 254 nm appear as dark zones, provided the layer contains a fluorescence indicator (fluorescence quenching). The TLC Visualizer 2 is CAMAG’s imaging and documentation system operated with visionCATS. visionCATS enables an image-based evaluation of chromatograms for quantitative evaluation. The strength of the electronic image acquisition is the overview of the complete chromatogram.
The spectral range of the CAMAG TLC Scanner 4 is 190–900 nm. Reflected light is measured either in the absorbance or in the fluorescence mode. From the acquired data quantitative results are computed with high precision and spectral selectivity.
With the TLC Scanner 4 absorption spectra can be recorded. The strengths of classical densitometry as compared with image evaluation are spectral selectivity and the higher precision of quantitative determinations.
Through the pioneering concept of hyphenating High-Performance Thin-Layer Chromatography with Mass Spectrometry unequivocal substance identification is possible. The CAMAG TLC-MS Interface 2 can be installed plug & play with any LC-MS system without adjustments or mass spectrometer modifications. Depending on the MS system, a substance can be identified within a minute via its mass spectrum, or for an unknown substance zone, the respective sum formula can be obtained. Furthermore, interesting zones can be eluted into vials for further investigations with, e.g., NMR, (ATR-)FTIR, ESI-MS, and MALDI-MS.
At Kovalent we strive to establish and develop long-term partnerships with our customers. When we use the word “partnership” we mean that Kovalent becomes an integral part of our customers’ projects, where we are expected to fully understand their application needs. We aim to make Kovalent our customers’ first and natural choice, and this ambition permeates our entire organisation from initial contact to finished and delivered product.
Genetec is a member of the same family of companies as Kovalent. Both companies share the same set of values and are in many ways integrated with each other. The main difference between the two companies are their products. Kovalent is involved in system solutions including instrumentation and Genetec is mainly working with consumables. The focus of Genetec is chromatography accessories. You are welcome to visit their state of the art web shop: genetec.se