Total Fat Determination in Foodstuffs

Ethos X for Fat Determination


Total Fat Determination in Foodstuffs

Traditional fat extraction methods are very labor intensive and time-consuming. Our ETHOS X performs determination of fat content in milk products simultaneously in just 90 minutes, having equivalent results with those obtained by reference fat determination methods.

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Total Determination of fat content in just 90 minutes!

Traditional methods lead to the use of large amounts of solvents to remove fat from a sample matrix, resulting in low cost-effectiveness.
As an example, conventional fat extraction of pretreated cheese usually requires 3 hours and over 100 mL of solvent per sample. The standard fat extraction methods are not efficient in matter of cost and time, also exposing laboratory technicians to dangerous solvents.
To overcome these limitations, Milestone has developed a fast yet accurate microwave-based technology, which allows the chemist to carry out the simultaneous determination of fat content in 12 milk and milk products samples in just 90 minutes, with equivalent results of those obtained with reference techniques like Rose-Gottlieb method, Weibull-Stoldt or Schmid-Bondzynski-Ratzlaff method. The main advantages of this technology include:

The analytical procedure for The analytical procedure for flavors and fragrances extraction from plants or spices comprises two steps: extraction by steam distillation or hydro-distillation, and analysis by GC or GC-MS. While the analysis step requires just few minutes, extraction takes several hours, using a standard procedure based on the Clevenger (1928 year!).
To address these limitations, Milestone has developed the new ETHOS X for the solvent-less extraction of natural products. It takes advantage of the unique microwave selective heating mechanism. The internal heating of the water within the sample distends its cells and leads to rupture of the glands and oleiferous receptacles. This process thus frees essential oil, and the flavors extracts is evaporated by the in-situ water of the plant material.

  • 12 samples simultaneously

  • 75% solvent reduction

  • Up to 10 gram of sample amount

  • Combined hydrolysis and extraction

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

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031-709 8186

Perfomances Comparison

The traditional fat extraction methods are very labour intensive and time-consuming.
Compare the performances of the ETHOS X microwave-based technology to those of the Reference Method

A New Method

The ETHOS X determines total fat in milk and milk products as a result of two working steps. In the first step sample hydrolysis and extraction take place simultaneously at relatively elevated temperature and pressure conditions. In the second step a known aliquot of the solvent with the extracted fat is evaporated allowing the chemist to weigh and measure the fat content.

Hydrolysis & Extraction

Up to 12 samples can be analyzed simultaneously, with a typical sample amount up to 10 gram. 10 mL of diluted acid and 25 mL of solvent are used for most matrices. The combined hydrolysis and extraction process takes place at 125°C and requires approximately 50 minutes or less.
Temperature is monitored and controlled in each and every vessel by using a unique combination of fiber optic and infrared temperature sensors. The ETHOS X built-in magnetic stirrer assures an even heating and temperature uniformity in all vessels, as well as intensive mixing between the aqueous and the solvent phases.

Typical combined hydrolysis and extraction temperature profile. Acid: diluted sulfuric acid or diluted hydrochloric acid. Solvent: cycle-hexane or petroleum ether.

Solvent Evaporation

A measured aliquot of the solvent with the extracted fat is evaporated in disposable cups, located in a dedicated rotor, in just 15 minutes.
The evaporation process takes place under controlled temperature and vacuum conditions, to avoid the thermal degradation of the fat content. At the completion of the evaporation, the fat content is immediately weighed on a balance which is directly interfaced with the ETHOS X, taking advantage of the extremely short cooling time of the disposable cups. The instrument operating software automatically calculates the fat content and all the statistics associated with the analysis of the 12 samples analyzed

Typical evaporation temperature profile.

High Technical Performance

Microwave hardware

The ETHOS X microwave cavity has a volume in excess of 70 liters, by far the largest currently available. With an installed power of 1900 Watt, the ETHOS X is the most powerful microwave platform system available for extraction. A rotating diffuser evenly distributes the microwaves throughout the cavity. A magnetic stirrer is built-in in the ETHOS X system. It is designed to ensure vigorous stirring of the solutions in all vessels. Finally, the ETHOS X actually acts as a fume hood, as it incorporates a powerful exhaust system.

User Interface

The new Milestone ETHOS X is equipped with the most advanced yet easy to use reaction temperature and pressure sensors for complete quality control of the extraction conditions. It is controlled via a compact terminal with an easy-to-read, bright, full-color, touchscreen display. The terminal runs a completely new user-friendly, icon-driven, multi-language software to provide easy control of the microwave run.

Microwave Green Extraction of Natural Products

Ethos X for Natural Products


Microwave Green Extraction of Natural Products

Available in two different and interchangeable configurations, for fragrances and for flavors, the ETHOS X takes advantage of the unique microwave microwave hydrodiffusion and gravity technique for high-quality essential oils extraction.

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Wish to get high-quality essential oils?

The analytical procedure for The analytical procedure for flavors and fragrances extraction from plants or spices comprises two steps: extraction by steam distillation or hydro-distillation, and analysis by GC or GC-MS. While the analysis step requires just few minutes, extraction takes several hours, using a standard procedure based on the Clevenger (1928 year!).
To address these limitations, Milestone has developed the new ETHOS X for the solvent-less extraction of natural products. It takes advantage of the unique microwave selective heating mechanism. The internal heating of the water within the sample distends its cells and leads to rupture of the glands and oleiferous receptacles. This process thus frees essential oil, and the flavors extracts is evaporated by the in-situ water of the plant material.

  • Fast, Solvent-less extraction

  • No work-up

  • High extracts purity

  • No thermal degradation

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

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031-709 8186

Testimonal

INRA AGROSCIENCE NATIONAL RESEARCH INSTITUTE

Prof. Farid Chemat, University of Avignon, France

Prof. Farid Chemat is full professor of chemistry and director of Green Extraction Laboratory where 200 international scientists deal with innovative techniques.


Choose your preferred setup

Two Different Configurations

Fragrances Extraction

The Fragrances configuration for ETHOS X is based on the solvent-free microwave extraction (SFME) technology for the rapid extraction of essential oils from aromatic herbs, spices and dry seeds, developed at the Laboratory of Chemistry of Natural Substances and Food Sciences at the Université de la Réunion, France (European patents EP 1 439 218, EP 1 618 798 and EP 1 629 725). The ETHOS X performs solvent-free microwave extraction at atmospheric pressure of fragrances, such as essential oils, in plant material. The method involves placing the sample in the microwave reactor, without any added solvent or water. A cooling system outside the microwave continuously condenses the vapors which are collected in a dedicated fragrances glassware. The excess of water is refluxed back to the extraction vessel to restore the in-situ water of the sample.
Once the essential oils have been extracted, they can be analyzed directly by GC-MS without any preliminary clean-up or solvent exchange steps. High quality fragrances are obtained without residual solvents, contaminants and artifacts.

Discover yields

Flavors Extraction

The Flavors configuration is based on the microwave hydro-diffusion and gravity technology (MHG), a new and green method of essential oils extracted from different kinds of aromatic plants, which led to the grant of the European patent EP 1 955 749. The microwave hydro-diffusion and gravity technology (MHG) is an original “upside down” microwave alembic, combining microwave heating and earth gravity at atmospheric pressure. This technology allows the flavors to diffuse outside the plant material, dropping by earth gravity out of the microwave reactor. A cooling system outside the microwave oven continuously condenses the extract. The extraction process takes place without the need of distillation and evaporation, some of the most energy demanding operations commonly required. Whereas traditional processes are carried out in 3 hours or more, solvent-free microwaves hydro-diffusion plus gravity requires less than 30 minutes.
Once the essential oils have been extracted, they can be analyzed directly by GC-MS without any preliminary clean-up or solvent exchange steps. The ETHOS X in the Flavors configuration allows the microwave extraction of natural flavoring substances and non-volatile compounds such as pigments, flavonoids and carotenoids.

Discover yields

Unique Microwave Platform

The new Milestone ETHOS X microwave cavity has a volume of 70 liters, by far the largest currently available, allowing the user to configure glass reactors from 2 up to 12 liters of volume.

2, 5 and 12 liters glass reactors for fragrances and flavors

Highest Microwave Power

The ETHOS X is equipped with two 950 Watt magnetrons for a total of 1900 Watt making it the most powerful microwave platform system available for extraction.

Best Extraction Control

 The ETHOS X is equipped with the most advanced yet easy to use contact-less temperature sensor for a complete control of the extraction process.

Build-In Exhaust System

The ETHOS X acts as a fume hood, as it incorporates a powerful exhaust system, which cools the reactors.

New User-friendly Software

Compact terminal with an easy-to-read, bright, full-color, touchscreen display. The terminal runs a completely new icon-driven, multi-language software.

Advanced Microwave Extraction System for Environmental Laboratories

Ethos X Enviromental


Advanced Microwave Extraction System for Environmental Laboratories

Microwave-assisted solvent extraction offers superior lab efficiency in the determination of organic pollutants with microwave green extraction technique. Typical applications include chlorinated pesticides, semi-volatile organics, PAHs, PCBs, chlorinated herbicides, phenols, organophosphorus pesticides, dioxins and furans

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Designed by you.
Developed for you.

Determination of organic pollutants in environmental matrices is a common task for thousands of laboratories worldwide, as it leads to controlling and protecting our environment from high levels of contaminants. This analysis is often done to evaluate the effectiveness of a remediation process, to assess the contamination in waste, in waste landfills and for general environmental monitoring. Therefore, every day environmental laboratories deal with several challenges to ensure high quality data and fast turnaround time while maintaining their competitiveness.
Extraction of pollutants from solid matrices is often performed with techniques that limit the productivity and have high running costs. Many laboratories still use the Soxhlet method that was developed in 1879!
Milestone listened to the needs of environmental laboratory professionals by developing the ETHOS X with the fastEX-24 rotor, which allows for simultaneous extraction of 24 samples in 40 minutes with minimal solvent usage. By using large volume disposable glass vials, the fastEX-24 rotor simplifies handling and allows to achieve lower detection limits.

  • High throughput. - 24 samples in 40 minutes. 

  • Superior return of investment - Substantial reduction in solvent. 

  • Simple handling - Disposable glass vials. 

  • Consintency & Reproducibility - Consistent and reproducible results. 

  • Safety & Reliability

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

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031-709 8186

Achieving lower detection limits
with higher sample amount

The ETHOS X with fastEX-24 rotor extracts up to 30 grams of sample with minimal solvent volume, helping analysts to accomplish their tasks.
The Milestone fastEX-24 rotor uses disposable glass vials, eliminating the need for cleaning and the possibility of memory effect between different runs. The 100 mL vials can accommodate the extraction of a large sample amount. The easy to handle and affordable cost of the vials leads to high productivity at a very low running cost.

24 samples in < 1 hour!

Easy of use with disposable glass vials

The ETHOS X operation is simple: samples are loaded into large volume disposable glass vials with appropriate solvent mixture, placed into the Weflon vessel and sealed. The unique Weflon material ensures homogeneous heating in all vessels, therefore increasing extraction efficiency even when different matrices are processed simultaneously, which enhances the lab's workflow. The accurate contactless temperature sensor ensures full control of the extraction cycle in all positions and displays realtime the temperature of all samples on the dedicated user interface. The 100-mL glass disposable vials accommodate large sample amounts of up to 30 g, enabling lower detection limits with minimal solvent volume. In addition, the memory effects often observed with polymer vessels or with other technologies are eliminated. This is a valuable feature for trace analysis of challenging species such as dioxins.

Producivity

Although new extraction technologies have been developed in recent years, they have fallen short on addressing the needs of environmental labs. The ETHOS X with fastEX-24 overcomes productivity limitations of other conventional approaches by enabling the extraction of a large number of samples in a single workday. Other technologies process one sample at a time sequentially, requiring long extraction cycles. The ETHOS X simultaneously processes 24 samples in only 40 minutes and eliminates cleaning steps by using disposable glass vials. The graphic below compares the typical daily productivity using the Milestone ETHOS X, Sequential Pressurized Liquid Extraction and Soxhlet.

Productivity based on 8 hours working shift. 24 samples requiring 40 minutes with the ETHOS X, 240 minutes with Sequential Pressurized Liquid Extraction and multiple hours with Soxhlet.


Consistency

Data quality and reliability are key features for environmental labs. The ETHOS X with the fastEX-24 rotor provides fast, accurate and precise analysis. Weflon vessels allow to simultaneously process various matrices ensuring optimal extraction efficiency. In addition, the unique design of the fastEX-24 rotor with its large volume disposable glass vials (100 mL) eliminates cross contamination and memory effect, providing reproducible results.

Milestone ETHOS X recovery study and comparison vs Soxhlet for various organic compounds.

Compliance

Several official methods describe the use of microwave closed-vessel technology to enhance the extraction efficiency of organic pollutants, such as US EPA 3546, ASTM and other national methods. The top-right table reports the typical conditions used in US EPA 3546. The ETHOS X with fastEX-24 further enhances the performance of microwave technology for the extraction of water-insoluble or slightly water-soluble organic compounds from soils, clays, sediments, sludges, and solid wastes. In fact, the ETHOS X with fastEX-24 can be used for solvent extraction of chlorinated pesticides, semi-volatile organics, PAHs, PCBs, chlorinated herbicides, phenols, organophosphorus pesticides, dioxins and furans.


Greater return on investment

The competitive nature of the environmental analysis market requires today's laboratories to have innovative solutions that provide faster turnaround times. With throughput capabilities of 24 samples in only 40 minutes, the ETHOS X with fastEX-24 reduces overall analysis costs by increasing productivity. The combination of lower solvent volumes, less maintenance needs, and the use of inexpensive disposable glass vials decreases the cost per sample to enhance your lab's competitiveness and profitability.

Flexibility

Elemental analysis by atomic spectroscopy is a standard analysis in environmental laboratories. The ETHOS X enables digestion of environmental matrices such as soils, sediments, wastewater in full compliance with official methods like US EPA. Several options are available to match the laboratory requirements.

Samples submitted for GC and HPLC analysis require pre-treatment

Ethos X for GC and HPLC Analysis


Samples submitted for GC and HPLC analysis require pre-treatment

The fundamental step in sample pretreatment is compound extraction from the sample matrix. This is the least evolved -many people still use the Soxhlet extraction method invented in 1879-, most error-prone step in the overall analytical procedure.

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Several configurations
to accomplish a wide range of applications

The use of microwave energy to accelerate solvent extraction procedures was first described by Ganzler et al. in 1984. These researchers used polar, microwave absorbing solvents or mixtures to extract crude fat from food and pesticides from soil matrices. Microwave extraction rates were found to be significantly faster, with recoveries comparable or superior to traditional Soxhlet extraction, and with a substantial reduction of the amount of solvent required.
New Milestone ETHOS X is an advanced microwave extraction system offering the best technology currently available for GC or GC-MS and HPLC analysis

  • Reliable GC or GC-MS analysis

  • High sample throughput

  • Low running costs

  • ● Efficiency and consistency

  • Simplicity

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
031-709 8186

The New Milestone Ethos X

Fast

Microwave extraction is completed in just few minutes. The Milestone ETHOS X can simultaneously process multiple samples in a matter of minutes. Microwave assisted extraction uses closed vessels to heat the extraction solvent above its atmospheric boiling point. The elevated temperature increases the solubility of the analytes of interest and lowers the viscosity of the solvent, allowing it to better penetrate the matrix. This reduced extraction times.

Easy

An important feature of the ETHOS X system is that it easily adapts to existing extraction chemistry. This is accomplished through the use of a unique, patented material, called Weflon. Without Weflon, extractions could only be performed with polar solvents (Acetone, Methanol, etc.) or mixtures of polar and non-polar solvents, because non-polar solvents are not heated by microwaves. Weflon eliminates this problem by acting as a coupling agent. Weflon stir bars are heated by microwaves and they subsequently transfer this heat to the non-polar solvent.

Effective and Consistent

Microwave extraction produces equal or higher analyte recoveries than older methods. Thanks to a combination of higher extraction temperatures, closed vessels, magnetic stirring, and precisely controllable temperature conditions, users of microwave extraction can expect a better quality of the results. The homogeneity of the microwave field and the precision of the temperature control are key elements in microwave extraction.

Economical

Lower solvent usage reduces the costs of solvent purchase and disposal. Microwave extractions are carried out in closed, sealed vessels. The matrix is constantly bathed with hot solvent in a self-promoting reflux action with no loss of volatile analytes or solvent. The use of closed vessels, with the speed and efficiency of microwave heating, allows extractions to be performed with significantly less solvent, without sacrificing accuracy or precision. The simplicity of the microwave technique reduces the attended labor time and increases overall productivity.

  • FastEX-24 configuration for extracting water insoluble or slightly water soluble organic compounds from soils, clays, sediments, sludges, and solid wastes.

  • SR-12 for hydrolysis and extraction for milk and milk products applications

  • RAR-12 for solvent evaporation for fat content determination.

  • SK-12 rotor for microwave solvent extraction.

Higher microwave power for faster vessel heating

Pressure-responsive door schematics.

Direct and contact-less temperature control in all vessels.

Largest Microwave Cavity

The ETHOS X microwave cavity has a volume in excess of 70 liters, by far the largest currently available. Why is this important? Firstly, extraction rotors with more sample positions can be accommodated, improving productivity. Secondly, the microwave unit is inherently much safer, because a larger cavity better contains gases escaping from vessels, should there be a sudden over pressurization.

Highest Microwave Power

Equipped with two 950 Watt magnetrons for a total of 1900 Watt, ETHOS X is the most powerful microwave extraction system available in the market today. The system additionally employs a rotating diffuser that evenly distributes the microwaves throughout the cavity. High power coupled with the diffuser enables very fast heating of high throughp

Pressure-Responsive Door

The ETHOS X's stainless steel door features an innovative opening and self-resealing mechanism. In case of a sudden over-pressurization of the cavity, the door slightly opens for rapid pressure release and the microwave power is instantaneously cut off. Immediately afterward, the door is pulled back, resealing the cavity. An automatic door locking system does not allow the user to open the ETHOS X door during the run.

Temperature and Pressure Sensors

The most advanced yet easy to use reaction sensors are installed for complete quality control of the extraction conditions. Direct temperature and pressure control are used in a single reference vessel. In all vessels, contact-less temperature is used combined with a contact-less pressure sensor that monitors and controls all vessels simultaneously, preventing any leakage or venting.

EasyCONTROL
Operating Software

The ETHOS X is controlled via a compact terminal with an easy-to-read, bright, full-color, touchscreen display. The terminal is provided with multiple USB and Ethernet ports for interfacing the instrument to external devices and to the local laboratory network. The terminal runs a completely new user-friendly, icondriven, multi-language software to provide easy control of the microwave run. Simply recall a previously stored method or create a new one, press ‘START’ and the system will automatically follow the user defined temperature utilising a sophisticated PID algorithm. Several applications, including all US EPA and ASTM methods available, are preloaded in the ETHOS X terminal. There is no need to input the number of samples or weights being extracted, as the software will automatically regulate the microwave power accordingly. This assures a consistent quality of extraction and simplifies the use of the instrument.

Environmentally clean extraction technique

Jasco SFE - Supercritical Fluid Extraction


Environmentally clean extraction technique

Supercritical fluid extraction (SFE) uses a supercritical fluid as an extraction solvent. Selective extraction can be made by exploiting the properties of differential solubility of target analytes in a wide variety of matrices.

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Jasco SFE

Supercritical Fluid Extraction employs a supercritical fluid, most commonly CO2, as the A mobile phase solvent for the extraction. The intrinsic low viscosity and high diffusivity of supercritical CO2 has rendered SFE a faster extraction and higher efficiency technique when compared to traditional liquid extraction. This provides faster flow rates and thus faster extraction times without the requirement for a higher pressure system. The addition of a co-solvent to the CO2 flow can help tune the strength further. The extraction oven can offer temperatures up to 100°C. Lastly after the extraction vessel is the back pressure regulator, which provides the back pressure requirement to keep the CO2 supercritical, and is an integral part of the performance of the extraction.

SFE Benefits

  • 1. Selectivity

    CO2 polarity varies greatly depending upon the pressure it is exposed to. This makes CO2 a tunable solvent which allows the user to find the precise conditions for extraction of the compounds of interest while leaving unwanted compounds behind. This inherent trait of CO2 greatly reduces the need for post extraction cleanup that would be necessary with most solvent extractions.

  • 2. No residual solvents

    Given the gaseous state of CO2 at atmospheric conditions, the resulting extract does not require the long rotovap time needed to dry solvent extracted analytes.

  • 3. Faster

    Given the high diffusivity and low viscosity of CO2 in its supercritical state the extractions typically take a fraction of the time compared to solvent extractions.

  • 4. Higher Yield

    Due to increased temperature and pressure, supercritical CO2 can penetrate many matrices that solvents cannot thereby allowing for greater surface area contact which in turn increases yield.

  • 5. Low Operating Cost

    Cost per extraction is significantly lower as the cost of CO2 is much lower than the equivalent amount of solvent.

  • 6. Environmentally Friendly

    Cost per extraction is significantly lower as the cost of CO2 is much lower than the equivalent amount of solvent.

  • When compared to Soxhlet extractions, supercritical fluid extractions have proven to be capable of providing extractions up to 25 times faster with equivalent recovery while using up to 30 times less solvent.

Product Specialist

Mats Grapne

"More than 30 years experience in the field of HPLC, Spectroscopy and other analytical equipments. Contact me if you have questions or need a quotation"

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0709-119301

Analytical SFE

  • ●  The analytical CO2 pump offers built-in peltier cooling to maintain a stable CO2 flow yielding excellent extraction time reproducibility. Automatic, built-in shut-off valves close the CO2 inlet and outlet and isolate the pumps for quick and simple priming when not pumping.

  • ●  The system can be configured for CO2 only or 1-10 co-solvents, 1-10 extraction vessels for the vessel volumes listed above and 1, 6, 12 or 54 fractions.

System             Extractions vessel            CO2 Flowrate

Analytical         1 ml, 5 ml, 10 ml               0,2-10 ml

Hybrid SFE

  • ●  The hybrid CO2 pump offers a flow range from 0.5 to 20 mL/min covering both analytical 10mL vessels up to semi-prep 100 mL vessels. Automatic, built-in shut-off valves close the CO2 inlet and outlet and isolate the pumps for quick and simple priming when not pumping.

  • ●  The system can be configured for CO2 only or 1-10 co-solvents, 1-10 extraction vessels for the vessel volumes listed above and 1, 6, 12 or 54 fractions.

System             Extractions vessel            CO2 Flowrate

Hybrid             10 ml, 50 ml, 100 ml         0,5-20 ml

Semi-Prep SFE

  • ●  The semi-prep CO2 pump offers a flow range from 3 to 50 mL/min covering 50 mL vessels up to 200 mL vessels (largest vessels not availible yet). Automatic, built-in shut-off valves close the CO2 inlet and outlet and isolate the pumps for quick and simple priming when not pumping.

  • ●  The system can be configured for CO2 only or 1-10 co-solvents, 1-10 extraction vessels for the vessel volumes listed above and 1, 6, 12 or 54 fractions.

System           Extractions vessel            CO2 Flowrate

Semi-Prep      50 ml, 100 ml, (200 ml)    3-50 ml

Preparative SFE

  • ●  The Prep CO2 pump offers a flow range from 5 to 150 mL/min covering 500 mL vessels up to 2L vessels (largest vessels not availible yet). Automatic, built-in shut-off valves close the CO2 inlet and outlet and isolate the pumps for quick and simple priming when not pumping.

  • ●  The system can be configured for CO2 only or 1-10 co-solvents, 1-10 extraction vessels for the vessel volumes listed above and 1, 6, 12 or 54 fractions.

System           Extractions vessel            CO2 Flowrate

Prep               (500 ml, 1 L, 2 L)               5-150 ml

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