Traditional fat extraction methods are very labor intensive and time-consuming. Our ETHOS X performs determination of fat content in milk products simultaneously in just 90 minutes, having equivalent results with those obtained by reference fat determination methods.

Traditional methods lead to the use of large amounts of solvents to remove fat from a sample matrix, resulting in low cost-effectiveness.
As an example, conventional fat extraction of pretreated cheese usually requires 3 hours and over 100 mL of solvent per sample. The standard fat extraction methods are not efficient in matter of cost and time, also exposing laboratory technicians to dangerous solvents.
To overcome these limitations, Milestone has developed a fast yet accurate microwave-based technology, which allows the chemist to carry out the simultaneous determination of fat content in 12 milk and milk products samples in just 90 minutes, with equivalent results of those obtained with reference techniques like Rose-Gottlieb method, Weibull-Stoldt or Schmid-Bondzynski-Ratzlaff method. The main advantages of this technology include:

The analytical procedure for The analytical procedure for flavors and fragrances extraction from plants or spices comprises two steps: extraction by steam distillation or hydro-distillation, and analysis by GC or GC-MS. While the analysis step requires just few minutes, extraction takes several hours, using a standard procedure based on the Clevenger (1928 year!).
To address these limitations, Milestone has developed the new ETHOS X for the solvent-less extraction of natural products. It takes advantage of the unique microwave selective heating mechanism. The internal heating of the water within the sample distends its cells and leads to rupture of the glands and oleiferous receptacles. This process thus frees essential oil, and the flavors extracts is evaporated by the in-situ water of the plant material.

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

The traditional fat extraction methods are very labour intensive and time-consuming.
Compare the performances of the ETHOS X microwave-based technology to those of the Reference Method

The ETHOS X determines total fat in milk and milk products as a result of two working steps. In the first step sample hydrolysis and extraction take place simultaneously at relatively elevated temperature and pressure conditions. In the second step a known aliquot of the solvent with the extracted fat is evaporated allowing the chemist to weigh and measure the fat content.

Hydrolysis & Extraction

Typical combined hydrolysis and extraction temperature profile. Acid: diluted sulfuric acid or diluted hydrochloric acid. Solvent: cycle-hexane or petroleum ether.

Solvent Evaporation

Typical evaporation temperature profile.

The ETHOS X microwave cavity has a volume in excess of 70 liters, by far the largest currently available. With an installed power of 1900 Watt, the ETHOS X is the most powerful microwave platform system available for extraction. A rotating diffuser evenly distributes the microwaves throughout the cavity. A magnetic stirrer is built-in in the ETHOS X system. It is designed to ensure vigorous stirring of the solutions in all vessels. Finally, the ETHOS X actually acts as a fume hood, as it incorporates a powerful exhaust system.

The new Milestone ETHOS X is equipped with the most advanced yet easy to use reaction temperature and pressure sensors for complete quality control of the extraction conditions. It is controlled via a compact terminal with an easy-to-read, bright, full-color, touchscreen display. The terminal runs a completely new user-friendly, icon-driven, multi-language software to provide easy control of the microwave run.

Available in two different and interchangeable configurations, for fragrances and for flavors, the ETHOS X takes advantage of the unique microwave microwave hydrodiffusion and gravity technique for high-quality essential oils extraction.

The analytical procedure for The analytical procedure for flavors and fragrances extraction from plants or spices comprises two steps: extraction by steam distillation or hydro-distillation, and analysis by GC or GC-MS. While the analysis step requires just few minutes, extraction takes several hours, using a standard procedure based on the Clevenger (1928 year!).
To address these limitations, Milestone has developed the new ETHOS X for the solvent-less extraction of natural products. It takes advantage of the unique microwave selective heating mechanism. The internal heating of the water within the sample distends its cells and leads to rupture of the glands and oleiferous receptacles. This process thus frees essential oil, and the flavors extracts is evaporated by the in-situ water of the plant material.

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

INRA AGROSCIENCE NATIONAL RESEARCH INSTITUTE

Prof. Farid Chemat, University of Avignon, France

Prof. Farid Chemat is full professor of chemistry and director of Green Extraction Laboratory where 200 international scientists deal with innovative techniques.

Fragrances Extraction

Flavors Extraction

The new Milestone ETHOS X microwave cavity has a volume of 70 liters, by far the largest currently available, allowing the user to configure glass reactors from 2 up to 12 liters of volume.

Highest Microwave Power

 The ETHOS X is equipped with the most advanced yet easy to use contact-less temperature sensor for a complete control of the extraction process.

Build-In Exhaust System

New User-friendly Software

Microwave-assisted solvent extraction offers superior lab efficiency in the determination of organic pollutants with microwave green extraction technique. Typical applications include chlorinated pesticides, semi-volatile organics, PAHs, PCBs, chlorinated herbicides, phenols, organophosphorus pesticides, dioxins and furans

Determination of organic pollutants in environmental matrices is a common task for thousands of laboratories worldwide, as it leads to controlling and protecting our environment from high levels of contaminants. This analysis is often done to evaluate the effectiveness of a remediation process, to assess the contamination in waste, in waste landfills and for general environmental monitoring. Therefore, every day environmental laboratories deal with several challenges to ensure high quality data and fast turnaround time while maintaining their competitiveness.
Extraction of pollutants from solid matrices is often performed with techniques that limit the productivity and have high running costs. Many laboratories still use the Soxhlet method that was developed in 1879!
Milestone listened to the needs of environmental laboratory professionals by developing the ETHOS X with the fastEX-24 rotor, which allows for simultaneous extraction of 24 samples in 40 minutes with minimal solvent usage. By using large volume disposable glass vials, the fastEX-24 rotor simplifies handling and allows to achieve lower detection limits.

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

The ETHOS X with fastEX-24 rotor extracts up to 30 grams of sample with minimal solvent volume, helping analysts to accomplish their tasks.
The Milestone fastEX-24 rotor uses disposable glass vials, eliminating the need for cleaning and the possibility of memory effect between different runs. The 100 mL vials can accommodate the extraction of a large sample amount. The easy to handle and affordable cost of the vials leads to high productivity at a very low running cost.

24 samples in < 1 hour!

The ETHOS X operation is simple: samples are loaded into large volume disposable glass vials with appropriate solvent mixture, placed into the Weflon vessel and sealed. The unique Weflon material ensures homogeneous heating in all vessels, therefore increasing extraction efficiency even when different matrices are processed simultaneously, which enhances the lab's workflow. The accurate contactless temperature sensor ensures full control of the extraction cycle in all positions and displays realtime the temperature of all samples on the dedicated user interface. The 100-mL glass disposable vials accommodate large sample amounts of up to 30 g, enabling lower detection limits with minimal solvent volume. In addition, the memory effects often observed with polymer vessels or with other technologies are eliminated. This is a valuable feature for trace analysis of challenging species such as dioxins.

Producivity

Productivity based on 8 hours working shift. 24 samples requiring 40 minutes with the ETHOS X, 240 minutes with Sequential Pressurized Liquid Extraction and multiple hours with Soxhlet.

Data quality and reliability are key features for environmental labs. The ETHOS X with the fastEX-24 rotor provides fast, accurate and precise analysis. Weflon vessels allow to simultaneously process various matrices ensuring optimal extraction efficiency. In addition, the unique design of the fastEX-24 rotor with its large volume disposable glass vials (100 mL) eliminates cross contamination and memory effect, providing reproducible results.

Milestone ETHOS X recovery study and comparison vs Soxhlet for various organic compounds.

Several official methods describe the use of microwave closed-vessel technology to enhance the extraction efficiency of organic pollutants, such as US EPA 3546, ASTM and other national methods. The top-right table reports the typical conditions used in US EPA 3546. The ETHOS X with fastEX-24 further enhances the performance of microwave technology for the extraction of water-insoluble or slightly water-soluble organic compounds from soils, clays, sediments, sludges, and solid wastes. In fact, the ETHOS X with fastEX-24 can be used for solvent extraction of chlorinated pesticides, semi-volatile organics, PAHs, PCBs, chlorinated herbicides, phenols, organophosphorus pesticides, dioxins and furans.

Greater return on investment

Flexibility

The fundamental step in sample pretreatment is compound extraction from the sample matrix. This is the least evolved -many people still use the Soxhlet extraction method invented in 1879-, most error-prone step in the overall analytical procedure.

The use of microwave energy to accelerate solvent extraction procedures was first described by Ganzler et al. in 1984. These researchers used polar, microwave absorbing solvents or mixtures to extract crude fat from food and pesticides from soil matrices. Microwave extraction rates were found to be significantly faster, with recoveries comparable or superior to traditional Soxhlet extraction, and with a substantial reduction of the amount of solvent required.
New Milestone ETHOS X is an advanced microwave extraction system offering the best technology currently available for GC or GC-MS and HPLC analysis

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

Fast

Easy

Effective and Consistent

Economical

Largest Microwave Cavity

Highest Microwave Power

Pressure-Responsive Door

Temperature and Pressure Sensors

The ETHOS X is controlled via a compact terminal with an easy-to-read, bright, full-color, touchscreen display. The terminal is provided with multiple USB and Ethernet ports for interfacing the instrument to external devices and to the local laboratory network. The terminal runs a completely new user-friendly, icondriven, multi-language software to provide easy control of the microwave run. Simply recall a previously stored method or create a new one, press ‘START’ and the system will automatically follow the user defined temperature utilising a sophisticated PID algorithm. Several applications, including all US EPA and ASTM methods available, are preloaded in the ETHOS X terminal. There is no need to input the number of samples or weights being extracted, as the software will automatically regulate the microwave power accordingly. This assures a consistent quality of extraction and simplifies the use of the instrument.

Supercritical fluid extraction (SFE) uses a supercritical fluid as an extraction solvent. Selective extraction can be made by exploiting the properties of differential solubility of target analytes in a wide variety of matrices.

Supercritical Fluid Extraction employs a supercritical fluid, most commonly CO2, as the A mobile phase solvent for the extraction. The intrinsic low viscosity and high diffusivity of supercritical CO2 has rendered SFE a faster extraction and higher efficiency technique when compared to traditional liquid extraction. This provides faster flow rates and thus faster extraction times without the requirement for a higher pressure system. The addition of a co-solvent to the CO2 flow can help tune the strength further. The extraction oven can offer temperatures up to 100°C. Lastly after the extraction vessel is the back pressure regulator, which provides the back pressure requirement to keep the CO2 supercritical, and is an integral part of the performance of the extraction.

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System             Extractions vessel            CO2 Flowrate

Analytical         1 ml, 5 ml, 10 ml               0,2-10 ml

System             Extractions vessel            CO2 Flowrate

Hybrid             10 ml, 50 ml, 100 ml         0,5-20 ml

System           Extractions vessel            CO2 Flowrate

Semi-Prep      50 ml, 100 ml, (200 ml)    3-50 ml

System           Extractions vessel            CO2 Flowrate

Prep               (500 ml, 1 L, 2 L)               5-150 ml