Flexible UHPLC with online SPE front end

Flexible UHPLC with online SPE front end

This system offers all the basic advantages of online SPE to increase assay performance. It minimizes SPE sample preparation steps like, SPE sample collection, drying/evaporation and LC reconstitution steps giving a faster SPE process with reduced variations.

Specification Sheet

Brochure

System Description

Beside this the High-Pressure Dispenser (HPD) is capable to deliver controlled and precise flow rates up to back pressures of 250 bar allowing to use SPE cartridges packed with 10 µm particles. This results in efficient SPE sample clean up and reduced LC-MS ionization suppression. Also, with online SPE the highest assay sensitivity is achieved with the minimum of sample being prepared (no sample losses) and with full traceability of the sample prep.

With the Solvent Selector in the SPH1299 pumps and the optional Column Selector in the Mistral™ it also allows you to run multiple batches under different LC conditions. You can even choose to do direct injection or to include the extraction, without reconfiguring your system hardware! The mix and dilute routine will be handled by the innovative and flexible PAL Autosampler. Sample preparation steps such as adding internal standard can also be performed. The system is equipped with a single clamp ACE module for the cartridges and allows sequential work. Times of cycles are about 8-10 minutes depending on the HPLC runtime, SPE sample prep time is about 4 minutes per sample. The system is upgradable to a higher throughput version with two clamps. This machine is ideal as a starting system for the full automation of sample prep with LC-MS analysis.

The customer can choose between two different ACE modules depending on the cartridges, he would like to work with. There is one option for 250 bar cartridges and one for 1000 bar cartridges available.

CHRONECT Symbiosis is controlled by CHRONOS Symbiosis, a software platform developed by Axel Semrau®. CHRONOS controls the entire system in an efficient and intelligent way. CHRONOS organizes the connection to the LC-MS. All CHRONECT Symbiosis systems process multiple batches/assays fast and reliably with the highest analytical performance, fully unattended.

System Configuration

Two systems in one: Automated HPLC injections and SPE sample prep for HPLC-MS analysis

● SPH1299 UHPLC gradient pump with 2x2 solvent selections
● ACE single clamp 250 or 1000 bar, 3x UHPLC valves
● HPD Mix 1x SSM / Organizer, Mistral Cool
● PAL LSI Autosampler with second valve
● CHRONOS Symbiosis for control

 

Product Specialist

Dag Dacke

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
070-7319009
  • Features

    ● Fully automated and unattended switching between HPLC-MS and SPE-HPLC-MS
    ● Minimal or no manual sample pre-treatment steps by applying disposable SPE cartridges
    ● Reduced LC-MS ionization suppression and sample carry-over
    ● Controlled sample prep SPE flow-rates (10 - 5000 µL/min) with SPE pressures up to 250 bar
    ● SPE sample clean-up and online-SPE-LC-MS/MS analysis in sequence
    ● Completely GLP compliant system, SPE sample preparation fully integrated with (U)HPLC-MS systems
    ● Injection volume variable, standard: 100 µL
    ● Upgradable to either CHRONECT Symbiosis Plus or Advanced systems
    ● Automatic UHPLC solvent compressibility compensation and automatic purge & prime functions
    ● Fast Wash of syringe, aspire & dispense
    ● Cost reduction of SPE solvents and sample collection vials vs. manual SPE
    ● Internal standard addition

  • With addittional options

    ● Mistral CS Cool UHPLC for easy column handling
    ● Sample cooling with Peltier Stacks (4 to 40 °C)
    ● Solvent Module with 3 extra solvents for PAL Sampler
    ● Higher capacity with more racks
    ● Better mixing with vortexer or agitator
    ● Integration into workflows with 2D Barcode Reader

    Many more options are available...

Flexible UHPLC with online SPE front end for research & development

CHRONECT Symbiosis Plus -NEW


Flexible UHPLC with online SPE front end for research & development

During method development, you will receive direct feedback and can calculate what the sample prep recoveries are for each optimization step.

Specification Sheet

Brochure

System Description

This system offers all the basic advantages of online SPE to increase assay performance. It minimizes SPE sample preparation steps like, SPE sample collection, drying/evaporation and LC reconstitution steps giving a faster SPE process with reduced variations. Beside this the High-Pressure Dispenser (HPD) is capable to deliver controlled and precise flow rates up to back pressures of 250 bar allowing to use SPE cartridges packed with 10 µm particles. This results in efficient SPE sample clean up and reduced LC-MS ionization suppression. Also, with online SPE the highest assay sensitivity is achieved with the minimum of sample being prepared (no sample losses) and with full traceability of the sample prep.

With the Solvent Selector in the SPH1299 pumps and the optional Column Selector in the Mistral™ it also allows you to run multiple batches under different LC conditions. You can even choose to do direct injection or to include the extraction, without reconfiguring your system hardware! The mix and dilute routine will be handled by the innovative and flexible PAL Autosampler. Sample preparation steps such as adding internal standard can also be performed. The system is equipped with a single clamp ACE module for the cartridges and allows sequential work. Times of cycles are about 8-10 minutes depending on the HPLC runtime, SPE sample prep time is about 4 minutes per sample. The system is upgradable to a higher throughput version with two clamps. This machine is ideal as a starting system for the full automation of sample prep with LC-MS analysis.

The customer can choose between two different ACE modules depending on the cartridges, he would like to work with. There is one option for 250 bar cartridges and one for 1000 bar cartridges available.

CHRONECT Symbiosis is controlled by CHRONOS Symbiosis, a software platform developed by Axel Semrau®. CHRONOS controls the entire system in an efficient and intelligent way. CHRONOS organizes the connection to the LC-MS. All CHRONECT Symbiosis systems process multiple batches/assays fast and reliably with the highest analytical performance, fully unattended.

 

System Configuration

Two systems in one: Automated HPLC injections and SPE sample prep for HPLC-MS analysis

● SPH1299 UHPLC gradient pump with 2x2 solvent selections
● SPH1299 UHPLC pump
● ACE dual clamp 250 bar, 4x UHPLC valves
● HPD Mix 1x SSM / Organizer, Mistral Cool
● PAL LSI Autosampler with second valve
● CHRONOS Symbiosis for control

Product Specialist

Dag Dacke

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
070-7319009
  • Features

    ● Fully automated and unattended switching between HPLC-MS and SPE-HPLC-MS
    ● Concurrent SPE sample clean-up and online-SPE-LC-MS analysis, giving zero sample prep time
    ● Standardized and fast SPE method development
    ● Easy to optimize SPE method protocols, reducing LC-MS ionization suppression and sample carry-over
    ● LC Peak Focussing mode applying Mixed Mode SPE protocols for efficient and more cleaner sample preparations
    ● Injection volume variable, standard: 100 µL
    ● Automatic UHPLC solvent compressibility compensation and automatic purge & prime functions
    ● Fast Wash of syringe, aspire & dispense
    ● Internal standard addition

  • With addittional options

    ● Mistral CS Cool UHPLC for easy column handling
    ● Sample cooling with Peltier Stacks (4 to 40 °C)
    ● Solvent Module with 3 extra solvents for PAL Sampler
    ● Higher capacity with more racks
    ● Better mixing with vortexer or agitator
    ● Integration into workflows with 2D Barcode Reader

    Many more options are available...

Powerful UHPLC with 1000 bar online SPE for routine application

CHRONECT Symbiosis Advanced -NEW


Powerful UHPLC with 1000 bar online SPE for routine application

This system is fully compatible with UHPLC columns up to 1000 bar using disposable PEEK SPE cartridges of 10 x 1 mm ID.

Specification Sheet

Brochure

System Description

As mentioned for the CHRONECT Symbiosis Basic and Plus system this system offers all the advantages of online SPE and the concurrent SPE sample preparation and LC-MS analysis. Additionally, this system is fully compatible with UHPLC columns up to 1000 bar using disposable PEEK SPE cartridges of 10 x 1 mm ID. Because of the reduced SPE cartridge volumes (8 µL, 10 x 1 mm ID) this system is compatible with the lower UHPLC flow rates without losing UHPLC resolution. Multicomponent UHPLC-MS analysis including fully automated sample prep within 2-4 min can be achieved.

The system offers all the advantages of online SPE to increase assay performance while reducing method development time and manual sample preparation steps. With the Solvent Selector in the SPH1299 pumps and the optional Column Selector in the NEW Mistral™ it also allows you to run multiple batches under different LC conditions. You can even choose to do direct injection or to include the extraction, without reconfiguring your system hardware! The mix and dilute routine will be handled by the innovative and flexible PAL Autosampler. Sample preparation steps such as adding internal standard can also be performed.

While the the clean-up is performed on the left cartridge, the SPE-UHPLC-MS can run simultaneously on the right cartridge. Forward and backward wash is possible, giving clean samples and high assay sensitivities running your multicomponent UHPLC analysis. Peak focussing can be realized with mixed mode SPE cartridges and an additional SPH1299 UHPLC gradient pump.

CHRONECT Symbiosis is controlled by CHRONOS Symbiosis, a software platform developed by Axel Semrau®. CHRONOS controls the entire system in an efficient and intelligent way and enhances sample throughput by performing overlapping processes. The cartridge will be prepared while the analytics are running. CHRONOS organizes the connection to the LC-MS. All CHRONECT Symbiosis Systems process multiple batches/assays fast and reliably with the highest analytical performance, fully unattended.

 

System Configuration

Two systems in one. Automated UHPLC injections and sample prep for UHPLC-MS analysis

● Two different and separate sample injection loops (for UHPLC injections & SPE cartridge sample loading)
● SPH1299 UHPLC gradient pump with 2 x 2 solvent selections
● ACE dual clamp 1000 bar, 4x UHPLC valves
● HPD MIX 1x SSM / Organizer, Mistral Cool
● PAL LSI Autosampler with second valve
● CHRONOS Symbiosis for control

Product Specialist

Dag Dacke

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
070-7319009
  • Features

    ● Fully automated and unattended switching between UHPLC-MS and SPE-UHPLC-MS
    ● Concurrent SPE sample clean-up and online-SPE-UHPLC-MS analysis, giving zero sample prep time
    ● LC Peak Focussing mode (with additional UHPLC pump) applying Mixed Mode SPE protocols for efficient and more cleaner sample preparations
    ● Sample loops with variable injection volume. Standard: 100 µL
    ● Automatic UHPLC solvent compressibility compensation and automatic purge & prime functions
    ● Fast Wash of syringe, aspire & dispense
    ● Internal standard addition

  • With addittional options

    ● Mistral CS Cool UHPLC for easy column handling
    ● Sample cooling with Peltier Stacks (4 to 40 °C)
    ● Solvent Module with 3 extra solvents for PAL Sampler
    ● Higher capacity with more racks
    ● Better mixing with vortexer or agitator
    ● Integration into workflows with 2D Barcode Reader

    Many more options are available...

Workstation for efficient determination of glyphosate in foodstuff

CHRONECT Workstation Glyphosate -NEW


Workstation for efficient determination of glyphosate in foodstuff

Manual steps during sample preparation are reduced to 10 minutes only.

Application Sheet

Brochure

System Description

This workstation by Axel Semrau® has been developed to determine glyphosate, glufosinate and AMPA in complex matrices as for example foodstuff. Manual steps during sample preparation are reduced to 10 minutes only, the entire remaining preparation and measurement is performed fully automated and interlaced in approx. 40 minutes.

Using online SPE-LC-MS/MS glyphosate is concentrated on specific cartridges. Combined with a Sciex Triple Quad 5500 system the determination limit for black tea, a very difficult matrix, is below 3 µg/kg. An excellent recovery between 86 and 96 % and a repeatability of ~ 10 % was measured for the detection of glyphosate, AMPA and glufosinate. This proves that the method is very reliable.

The CHRONECT Workstation Glyphosate consists of a XYZ robot with a centrifuge, vortexer and agitator, an online SPE unit with prepared methods and the specialized cartridges CHROSPE Glyphosate. Utilization of standard SPE cartridges for simple matrices, e.g. C8 or C18 phases, is possible. CHRONECT Workstation Glyphosate will be delivered with ready-for-use methods for different matrices.
Being a front-end solution, the workstation can be coupled to various LC-MS/MS systems. Direct injection, online SPE with or without derivatization of the sample are readily available on the device.,

System Configuration

● SPH1299 UHPLC pump
● ACE single clamp 250 or 1000 bar, 1x UHPLC valves
● HPD MIX 1x SSM / Organizer, Mistral column oven
● CHRONECT Symbiosis Autosampler with Park Station & tool change, 2 valves, centrifuge, vortexer, agitator, Fast Wash Module, Solvent Module, tray plate with 3 trays VT15 10/20 mL automatic tool change
● CHRONOS Symbiosis for control

 

Product Specialist

"Our expert in CTC PAL know-how and programming, and more than 20 years experience in the field of chromatography. Contact me if you have questions or need a quotation"

Dag Dacke

This email address is being protected from spambots. You need JavaScript enabled to view it.
070-7319009
  • Features

    ● High sample throughput, approx. 60 samples/ 24 hours 
    ● Ready-to-use methods and special cartridges for enrichment
    ● High level of automation, good recovery and precision, less manual work steps
    ● High sensitivity even in complex matrices
    ● All methods possible: online SPE, direct injection with or without derivatization
    ● Easy control and automatically optimized time schedules by CHRONOS Symbiosis

  • With addittional options

    ● Mistral CS Cool UHPLC for easy column handling
    ● Sample cooling with Peltier Stacks (4 to 40 °C)
    ● Solvent Module with 3 extra solvents for PAL Sampler
    ● Higher capacity with more racks
    ● Better mixing with vortexer or agitator
    ● Integration into workflows with 2D Barcode Reader

    Many more options are available...

KOVALENTS PHILOSOPHY

At Kovalent we strive to establish and develop long-term partnerships with our customers. When we use the word “partnership” we mean that Kovalent becomes an integral part of our customers’ projects, where we are expected to fully understand their application needs. We aim to make Kovalent our customers’ first and natural choice, and this ambition permeates our entire organisation from initial contact to finished and delivered product.

CONTACTS:

Kovalent AB
Hulda Lindgrens gata 6A
421 31 VÄSTRA FRÖLUNDA
Sweden
Phone: +46(0)31-709 81 80
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Org.nr: 556417-2194

SKANDINAVISKA GENETEC AB

Genetec is a member of the same family of companies as Kovalent. Both companies share the same set of values and are in many ways integrated with each other. The main difference between the two companies are their products. Kovalent is involved in system solutions including instrumentation and Genetec is mainly working with consumables. The focus of Genetec is chromatography accessories. You are welcome to visit their state of the art web shop: genetec.se