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Jasco FP-8250 Spectrofluorometer

Jasco FP-8250 Spectrofluorometer

The simplified design includes everything that is required for routine fluorescence measurements in a quality-control or teaching lab.

Simple and sensitive system which readily accommodates routine measurements and accessories, such as spectral scanning, quantitation, and temperature control.

Jasco FP-8250 Spectrofluorometer

The standard Auto-SCS and Auto-Gain features enable measurements to be obtained over a wide range of concentrations using only one method. Two graphical user interfaces are available: Spectra Manager™ cross-platform spectroscopy software that allows full system control of the instrument, separate from the data processing and analysis, and the intelligent remote module (iRM) with a color LCD touch screen.
  • ● High sensitivity S/N > 4,500 (RMS)

  • ● Dynamic range up to six digits
  • ● High speed scanning up to 20,000 nm/min
  • ● Wavelength range: 200 to 750 nm

  • ● Higher order diffraction band-pass filter (optional)

Product Specialist

Mats Grapne Kovalent

Mats Grapne

"More than 30 years experience in the field of HPLC, Spectroscopy and other analytical equipments. Contact me if you have questions or need a quotation"

FP-8050 Series Instrument Performance

Outstanding Sensitivity and Linear Dynamic Range

The high-throughput optical system and low noise signal processing of the FP-8050 series results in an extremely high signal-to-noise (S/N) performance up to 8,500:1 (RMS). In addition, the FP-8050 series has been enhanced to provide up to 7 orders of magnitude of linear dynamic range. As shown in the calibration curve for fluorescein, there is excellent linearity even for samples at very low concentrations.

Fluorescein spectra for a range of concentrations

Calibration curve for fluorescein
(Auto Sensitivity Control System (SCS) ON)

Higher-Order Light Cut-Off Filters

In order to remove peaks originating from higher-order diffracted light, cut-off filters appropriate to the measurement wavelength should be used. The FP-8050 Series (excl. FP-8250) include cut-off filters that are matched to the wavelength range and switched automatically

3D Spectra of Fluorescent Orange Color Plate.

Accurate Spectral Correction

Spectral correction should be made to any fluorometer that is used for defining accurate spectral data. All models in the FP-8050 Series can be spectrally corrected with a simple procedure that uses a range of calibration standards, including broad-spectrum standard light sources and Rhodamine B.

Auto Adjustment of Detector Gain and Sensitivity

The FP-8050 Series includes both Auto-Gain and Auto-Sensitivity Control System (SCS), which automatically adjust the detector gain and sensitivity for optimum measurement. Auto-Gain automatically adjusts the gain of the signal from the detector so that the S/N is optimized throughout the entire scan range for spectral measurement, and weak fluorescence spectral peak shapes can be observed against scattered light with a high S/N. The Auto-SCS allows the user to create calibration curves for a wide concentration range without having to manually change the instrument sensitivity settings.

Fluorescence spectra of quinine sulfate solution

Automatic Band-Pass Filter for Higher-Order Diffraction

The automatic band-pass filters remove peaks originating from higher-order diffracted light fr artifact-free spectral acquisition and analysis. The band-pass filters can also be used for spectral corrections, 3D measurements and quantum yield determination.

Automatic Accessory Recognition

Accessories are automatically recognized when installed in the instrument. Spectra Manager™ logs the accessory name and serial number; this information is saved in the data file for a complete record of the measurement.

Long-Life Light Source

Continuous output xenon arc lamps offer the greatest sensitivity. For the longest and highest performance, the FP-8050 series now includes a newly designed Xe arc lamp with prolonged lifetime. This new light source can be used up to 3x longer than previous lamps (maximum operating time: approximately 3000 hours), with long-term stable measurements, reduced maintenance and lower running costs.

Daily Monitoring of Instrument Performance

Validation program
The Validation program includes a full suite of tests to validate the total instrument performance. When executed, simple prompts guide the user on how to perform the tests. All instruments in the FP-8050 series include a mercury lamp as standard for wavelength calibration.

Daily Check program

The Daily Check is a less comprehensive performance check can be made at any time for continuous performance monitoring. After starting the Daily Check program, a timer is activated, and sample measurement is executed automatically after a predetermined stabilization time. The results are collated over time and a batch display lets the user review the ongoing performance of their instrument.

Instrument Activity Log

The instrument activity log is a useful alternative to the paper log books found in multi-user facilities. A record is maintained of user, duration of operation and lamp usage.

Overview FP-8050 Series Fluorometer

The FP-8050 Series of spectrofluorometers includes four different instruments that provide solutions for the broadest range of applications including QC, biomolecular structural studies, environmental monitoring and advanced materials science. The FP-8050 Series has many flexible options for academic or industrial research, teaching, or use in quality control labs. Users can have the greatest confidence in their measurements, with an optical bench specifically designed for the highest sensitivity, widest dynamic range, and exceptional spectral purity with automatic cut-off filters to exclude higher order diffraction.

The FP-8050 Series combines a compact design with the largest range of accessories together with Spectra Manager™ Suite, a comprehensive data platform that gives you complete control over measurement, analysis and data archiving. In addition to the standard analysis programs, JASCO has developed many different applications software for dedicated sample measurement.

Advanced Features of the FP-8050 Series

  • High-throughput optical system
  • Highest S/N performance
  • Wide dynamic range (up to 7 orders of magnitude)
  • Auto Gain and Auto Sensitivity Control System
  • Automatic cut-off filters for higher-order diffraction
  • Advanced digital signal processing
  • High speed scanning
  • Spectral bandwidth down to 1 nm

Extensive Breadth of Features and Accessories

  • Compact benchtop footprint
  • Precision temperature control accessories for liquids and solids
  • Polarizers allow for automatic anisotropy measurements
  • Integrating spheres for Quantum Yield Determination
  • Microplate Reader for rapid sample throughput
  • Microsampling accessories for small volume samples
  • Stopped-flow systems for monitoring fast kinetics
  • Spectra Manager™ software for control and data analysis
  • Flexible design for expanding capabilities as needs evolve

Versatility for a Wide Range of Applications

  • Protein dynamics
  • Quantitative analysis
  • Cellular membrane studies
  • Enzyme kinetics
  • Water quality monitoring
  • Quantum dot and probe design
  • Carbon nanostructures and 2D materials
  • Fluorescent tracking materials
  • Short lifetime phosphorescence and PHOLEDs