A powerful combination of performance, sensitivity and flexibility for biological, environmental and materials analysis.
The FP-8350 is an extremely sensitive spectrofluorometer that can be used in the broadest range of applications: biological, environmental, materials science, teaching labs, and core facilities.
Jasco FP-8350 Spectrofluorometer
Many features make this a simple-to-use instrument; automatic cut-off filters (included as standard) eliminate peaks due to second-order scatter, giving more confidence in artifact-free spectral measurement. Auto-Gain and Auto-SCS optimize the S/N for samples with large differences in concentration and fluorescence intensity, offering a dynamic range up to 7 orders of magnitude. This reduces the need for manual intervention of adjusting spectral band and sensitivity settings for on-scale measurement.
Single and multi-cell Peltier accessories provide exceptional temperature control for thermal studies such as molecular conformation and folding.
Two different sizes of integrating sphere (including LN2 cooled 120mm diam.) coupled with spectral correction provide accurate quantum yield determinations.
Automated broad wavelength polarizers can be used for a range of experiments including anisotropy for investigation of binding events. Rapid-kinetics and titration measurements can be automated with fully integrated stopped-flow and auto-titrator units. Solid samples can be measured with dedicated holders for powders, films, etc.
-
● High sensitivity S/N 8000:1 typical
-
● High resolution of 1.0 nm
-
● Wavelength range: 200 to 750 nm (900 nm optional)
Product Specialist
Mats Grapne
08-473 33 40
Fluorescein spectra for a range of concentrations
Calibration curve for fluorescein
(Auto Sensitivity Control System (SCS) ON)
Higher-Order Light Cut-Off Filters
In order to remove peaks originating from higher-order diffracted light, cut-off filters appropriate to the measurement wavelength should be used. The FP-8050 Series (excl. FP-8250) include cut-off filters that are matched to the wavelength range and switched automatically
3D Spectra of Fluorescent Orange Color Plate.
Auto Adjustment of Detector Gain and Sensitivity
The FP-8050 Series includes both Auto-Gain and Auto-Sensitivity Control System (SCS), which automatically adjust the detector gain and sensitivity for optimum measurement. Auto-Gain automatically adjusts the gain of the signal from the detector so that the S/N is optimized throughout the entire scan range for spectral measurement, and weak fluorescence spectral peak shapes can be observed against scattered light with a high S/N. The Auto-SCS allows the user to create calibration curves for a wide concentration range without having to manually change the instrument sensitivity settings.
Fluorescence spectra of quinine sulfate solution
Automatic Accessory Recognition
Accessories are automatically recognized when installed in the instrument. Spectra Manager™ logs the accessory name and serial number; this information is saved in the data file for a complete record of the measurement.
Daily Check program
The Daily Check is a less comprehensive performance check can be made at any time for continuous performance monitoring. After starting the Daily Check program, a timer is activated, and sample measurement is executed automatically after a predetermined stabilization time. The results are collated over time and a batch display lets the user review the ongoing performance of their instrument.
