A powerful combination of performance, sensitivity and flexibility for biological, environmental and materials analysis.
The FP-8350 is an extremely sensitive spectrofluorometer that can be used in the broadest range of applications: biological, environmental, materials science, teaching labs, and core facilities.
Many features make this a simple-to-use instrument; automatic cut-off filters (included as standard) eliminate peaks due to second-order scatter, giving more confidence in artifact-free spectral measurement. Auto-Gain and Auto-SCS optimize the S/N for samples with large differences in concentration and fluorescence intensity, offering a dynamic range up to 7 orders of magnitude. This reduces the need for manual intervention of adjusting spectral band and sensitivity settings for on-scale measurement.
Single and multi-cell Peltier accessories provide exceptional temperature control for thermal studies such as molecular conformation and folding.
Two different sizes of integrating sphere (including LN2 cooled 120mm diam.) coupled with spectral correction provide accurate quantum yield determinations.
Automated broad wavelength polarizers can be used for a range of experiments including anisotropy for investigation of binding events. Rapid-kinetics and titration measurements can be automated with fully integrated stopped-flow and auto-titrator units. Solid samples can be measured with dedicated holders for powders, films, etc.
● High sensitivity S/N 8000:1 typical
● High resolution of 1.0 nm
● Wavelength range: 200 to 750 nm (900 nm optional)
Product Specialist
Mats Grapne
"More than 30 years experience in the field of HPLC, Spectroscopy and other analytical equipments. Contact me if you have questions or need a quotation"
The high-throughput optical system and low noise signal processing of the FP-8050 series results in an extremely high signal-to-noise (S/N) performance up to 8,500:1 (RMS). In addition, the FP-8050 series has been enhanced to provide up to 7 orders of magnitude of linear dynamic range. As shown in the calibration curve for fluorescein, there is excellent linearity even for samples at very low concentrations.
Fluorescein spectra for a range of concentrations
Calibration curve for fluorescein (Auto Sensitivity Control System (SCS) ON)
Higher-Order Light Cut-Off Filters
In order to remove peaks originating from higher-order diffracted light, cut-off filters appropriate to the measurement wavelength should be used. The FP-8050 Series (excl. FP-8250) include cut-off filters that are matched to the wavelength range and switched automatically
3D Spectra of Fluorescent Orange Color Plate.
Accurate Spectral Correction
Spectral correction should be made to any fluorometer that is used for defining accurate spectral data. All models in the FP-8050 Series can be spectrally corrected with a simple procedure that uses a range of calibration standards, including broad-spectrum standard light sources and Rhodamine B.
Auto Adjustment of Detector Gain and Sensitivity
The FP-8050 Series includes both Auto-Gain and Auto-Sensitivity Control System (SCS), which automatically adjust the detector gain and sensitivity for optimum measurement. Auto-Gain automatically adjusts the gain of the signal from the detector so that the S/N is optimized throughout the entire scan range for spectral measurement, and weak fluorescence spectral peak shapes can be observed against scattered light with a high S/N. The Auto-SCS allows the user to create calibration curves for a wide concentration range without having to manually change the instrument sensitivity settings.
Fluorescence spectra of quinine sulfate solution
Automatic Band-Pass Filter for Higher-Order Diffraction
The automatic band-pass filters remove peaks originating from higher-order diffracted light fr artifact-free spectral acquisition and analysis. The band-pass filters can also be used for spectral corrections, 3D measurements and quantum yield determination.
Automatic Accessory Recognition
Accessories are automatically recognized when installed in the instrument. Spectra Manager™ logs the accessory name and serial number; this information is saved in the data file for a complete record of the measurement.
Long-Life Light Source
Continuous output xenon arc lamps offer the greatest sensitivity. For the longest and highest performance, the FP-8050 series now includes a newly designed Xe arc lamp with prolonged lifetime. This new light source can be used up to 3x longer than previous lamps (maximum operating time: approximately 3000 hours), with long-term stable measurements, reduced maintenance and lower running costs.
Daily Monitoring of Instrument Performance
Validation program The Validation program includes a full suite of tests to validate the total instrument performance. When executed, simple prompts guide the user on how to perform the tests. All instruments in the FP-8050 series include a mercury lamp as standard for wavelength calibration.
Daily Check program
The Daily Check is a less comprehensive performance check can be made at any time for continuous performance monitoring. After starting the Daily Check program, a timer is activated, and sample measurement is executed automatically after a predetermined stabilization time. The results are collated over time and a batch display lets the user review the ongoing performance of their instrument.
Instrument Activity Log
The instrument activity log is a useful alternative to the paper log books found in multi-user facilities. A record is maintained of user, duration of operation and lamp usage.
Overview FP-8050 Series Fluorometer
The FP-8050 Series of spectrofluorometers includes four different instruments that provide solutions for the broadest range of applications including QC, biomolecular structural studies, environmental monitoring and advanced materials science. The FP-8050 Series has many flexible options for academic or industrial research, teaching, or use in quality control labs. Users can have the greatest confidence in their measurements, with an optical bench specifically designed for the highest sensitivity, widest dynamic range, and exceptional spectral purity with automatic cut-off filters to exclude higher order diffraction.
The FP-8050 Series combines a compact design with the largest range of accessories together with Spectra Manager™ Suite, a comprehensive data platform that gives you complete control over measurement, analysis and data archiving. In addition to the standard analysis programs, JASCO has developed many different applications software for dedicated sample measurement.
Advanced Features of the FP-8050 Series
High-throughput optical system
Highest S/N performance
Wide dynamic range (up to 7 orders of magnitude)
Auto Gain and Auto Sensitivity Control System
Automatic cut-off filters for higher-order diffraction
Advanced digital signal processing
High speed scanning
Spectral bandwidth down to 1 nm
Extensive Breadth of Features and Accessories
Compact benchtop footprint
Precision temperature control accessories for liquids and solids
Polarizers allow for automatic anisotropy measurements
Integrating spheres for Quantum Yield Determination
Microplate Reader for rapid sample throughput
Microsampling accessories for small volume samples
Stopped-flow systems for monitoring fast kinetics
Spectra Manager™ software for control and data analysis
Flexible design for expanding capabilities as needs evolve