The miVac DNA is an ideal small vacuum concentrator for the molecular biology laboratory.

miVac DNA


All included in a compact format

The miVac DNA is an ideal small vacuum concentrator for the molecular biology laboratory.

Racks and Accessories

Brochure

System description

The miVac DNA is capable of removing low volumes of water and organic solvents from a variety of sample formats including tubes, microplates, vials and round bottom flasks. Making it an ideal concentrator for the busy molecular biology laboratory.

Key Features:

Built in high performance vacuum pump, Concentration chamber with electro-magnetic drive for quiet, maintenance free operation, Fixed angle aluminium rotor for 1.5 ml or 2 ml micro-centrifuge tubes, High displacement vacuum pump, Clear, acrylic lid to monitor progress
  • Simply position the system on the bench, connect the power lead and exhaust tube and look forward to faster, trouble-free concentration!
  • ● Concentration time and temperature are readily monitored on the large clear display
  • ● Parameter scan be easily set and selected using the friendly dial
  • ● Optimise concentration time
  • ● Faster than any other system in its class.

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
031-709 8186

Application Examples

Clinical and Diagnostic Testing

Evaporative sample preparation for forensic and diagnostic clinical tests must be performed scrupulously. Genevac systems are used by clinical biochemists and forensic scientists for a number of testing methodologies, such as vitamin D or K analysis, neonatal screening and forensic toxicology.

Mass Spectrometry (MS) is replacing radio-immunoassay (RIA) in Clinical biochemistry analysis, removing the need to use radioactive reagents. One of the Steroid assays performed by hospital clinical labs is for Vitamin D analysis where there is a need to identify the relative abundance of vitamin D2 and D3. Samples are typically extracted into Hexane, concentrated (e.g. using a miVac) and analysed by MS (avoiding ion suppression problems often seen with RIA).

Clinical and forensic toxicology investigations also utilise MS methodology, typically with solid phase extraction and evaporation as part of the sample preparation process. Blood and urine analysis can be utilised as part of a medico-legal investigation into drug-related deaths and drug-facilitated crimes and in the clinical assessment of alcohol/drug consumption. Hair analysis can also provide evidence over time of drug or alcohol abuse, as part of programme compliance for those participating in drug or alcohol dependency treatment or for workplace or health insurance screening. The EZ-2 has been used to streamline the sample preparation process whilst maintaining sample integrity and improving sensitivity.

Tandem MS is also used to identify metabolic disorders which include aminoacidemias, urea cycle disorders, organic acidurias, and fatty acid oxidation disorders. Samples can be presented as plasma, urine, and blood spots or urine on filter paper. Sample preparation techniques can include solvent extraction, acid derivitisation and evaporation prior to reconstitution in a suitable medium for injection to MS. Evaporation of butanolic HCl used for derivatisation using blowdown techniques results in corrosion of the system especially pins/needles/jets which can lead to contamination of samples and erroneous results. The EZ-2 with HCI resistance commonly used for these sample preparation stages. One example would be the test for CAT (carnitine acyl carnitine translocase deficiency) in neonates, an inborn error of metabolism. Lack of this enzyme prevents the body from converting fats into energy. Labs do the initial test on Guthrie cards (dried blood spots). The cards are punched and extracted with 200ul of Methanol. The supernatant is placed into microtitre plates and dried. Samples are then derivatised using approx 100ul 3N HCl in anhydrous Butanol and dried again. Finally, samples are re-suspended in Water & acetonitrile for analysis by MS.

miVac concentrators have been used by the Australian Sports Drug Testing Laboratory to improve the testing for synthetic insulin analogues in athletes' blood samples. Using the miVac's ability to control conditions during the concentration step the analyst has more confidence that they are seeing the true blood concentration of potential drugs of abuse in the athlete's sample.


Useful Papers:

  • Evaluation of the EZ-2 for Forensic Toxicology - Dr Eleanor Miller & Dr Simon Elliott, ROAR Forensics, UK. Evaluated the EZ-2 for use concentrating extracts from hair samples to detect alcohol and drugs of abuse metabolites entrained in the samples
  • Evaluation of Evaporative Sample Preparation Techniques for the Extraction of Drugs of Abuse from Urine. Describes the development of a new method for the pre- analytical extraction of drugs of abuse from urine samples pertaining to drug facilitated sexual assault cases. By Forensic Science Ireland (FSI).
  • Better Detection of Insulin Analogues - Great care is required when preparing the samples for analysis, this paper evaluates two possible methods (nitrogen blow down & vacuum concentration) and highlights the pitfalls. Study by Australian Sports Drug Testing Laboratory.

Contact us if you are interested in any of the above papers

DNA & Oligonucleotides

Genevac systems facilitate a wide range of DNA sample preparation and processing activities, ranging from straightforward small volume concentration of DNA pellets prior to PCR, through to high throughput processing of many purified DNA or oligonucleotide samples.  Where a dry sample of DNA is required, concentration to dryness in a centrifugal evaporator is the ideal method, presenting the DNA as a dry film in the tube of plate well.  Freeze drying of DNA samples is not recommended because the resultant DNA powder is easily blown about or moved by electrostatic charges.

DNA Concentration
Concentration of DNA from samples containing water or a mixture of water and alcohols is a simple process, which for most researchers requires the miVac DNA concentrator.  Concentration to remove a few hundred microlitres of alcohol from a DNA pellet takes approximately 10 minutes, or less.   This is one of the simplest applications of a Genevac system – in a miVac DNA concentrator, set the temperature, select the method for alcohols or water, and press start. 

Oligonucleotides & DNA Purification
The manufacture of oligonucleotides commonly has two steps where evaporation is required, evaporation of ammonia solutions following synthesis, and then subsequent evaporation of purified samples.   Protection of oligonucleotides from damage during drying is critical, especially where a tag or label has been attached to the DNA.  Two studies by Genevac users show that this can safely be achieved in a Genevac evaporator: 
  • Comparing Evaporators for Drying Oligonucleotides - Catherine McKeen, Eurogentec SA A Rapid,
  • Safe Technique for Drying Oligonucleotides - Dr Tim Watts, Wellcome Trust Human Genome Centre
Samples containing ammonia solution should be evaporated using Dri-Pure® (500g) to prevent sample loss and cross contamination.  When ammonia solution is subjected to a vacuum the ammonia degasses.  Degassing must be controlled or the resultant foam overflows the tube causing sample loss and cross contamination.  In the same way that you control degassing of a bottle of soda by opening it slowly, gentle application of vacuum is required, this coupled with the high rotors speeds of Dri-Pure keep every sample where it should be.  The latest versions of the EZ-2 come with such a method pre-programmed, and the method is simple to program on the HT-Series.
  
Where samples have been purified by reverse phase HPLC samples are presented in a mixture of water and methanol or acetonitrile.  The methods laid out in the Post Purification Sample Handling section should be followed.  The imbalance tolerance built into Genevac systems gives researchers confidence that eluates at different ends of the gradient will still dry down, and if there is too much imbalance the system will automatically shut down and tell the user.

Preparation of DNA Microarrays
For certain microarraying techniques concentrated samples of DNA are required.  To maintain such a concentration is difficult due to the volatile nature of the buffers holding the DNA, therefore samples should be made up to the required volume immediately prior to analysis.  A study by the Wellcome Trust Centre for Human Genetics in Oxford has shown that when an EZ-2 is used in this process the data quality returned by the system is significantly better, reducing the no call rate by 80% or more.

Modular range of centrifugal vacuum concentrators.

miVac Sample Concentrators


Modular range of centrifugal vacuum concentrators

miVac is a modular range of centrifugal vacuum concentrators and freeze driers capable of removing water and organic solvents from a variety of sample formats including tubes, microplates, and vials.

Racks and Accessories

Brochure

System description

Choose from the Duo Concentrator for low sample numbers or the Quattro Sample Concentrator for larger numbers. Combine with a Duo, Quattro or Scroll vacuum pump depending on the solvents being concentrated and add further options such as the unique SpeedTrap cold trap and vacuum controller.

Unique solid aluminium JetRotors and built-in special methods optimise the concentration of water and water mixtures, improving performance and reducing time.

The miVac SpeedTrap™ may also be used for freeze drying low volumes of water, and can be operated as a stand alone small volume freeze drier, or configured as a combined concentration / freeze drying workstation
  • ● Compact modular units
  • ● Easy to use
  • ● Precise temperature control
  • ● Heat & run timers
  • ● Novel frost free SpeedTrap
  • ● Unique solid aluminium rotors
  • ● Fast evaporation
  • ● Environmentally friendly

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
031-709 8186

Application Examples

Clinical and Diagnostic Testing

Evaporative sample preparation for forensic and diagnostic clinical tests must be performed scrupulously. Genevac systems are used by clinical biochemists and forensic scientists for a number of testing methodologies, such as vitamin D or K analysis, neonatal screening and forensic toxicology.

Mass Spectrometry (MS) is replacing radio-immunoassay (RIA) in Clinical biochemistry analysis, removing the need to use radioactive reagents. One of the Steroid assays performed by hospital clinical labs is for Vitamin D analysis where there is a need to identify the relative abundance of vitamin D2 and D3. Samples are typically extracted into Hexane, concentrated (e.g. using a miVac) and analysed by MS (avoiding ion suppression problems often seen with RIA).

Clinical and forensic toxicology investigations also utilise MS methodology, typically with solid phase extraction and evaporation as part of the sample preparation process. Blood and urine analysis can be utilised as part of a medico-legal investigation into drug-related deaths and drug-facilitated crimes and in the clinical assessment of alcohol/drug consumption. Hair analysis can also provide evidence over time of drug or alcohol abuse, as part of programme compliance for those participating in drug or alcohol dependency treatment or for workplace or health insurance screening. The EZ-2 has been used to streamline the sample preparation process whilst maintaining sample integrity and improving sensitivity.

Tandem MS is also used to identify metabolic disorders which include aminoacidemias, urea cycle disorders, organic acidurias, and fatty acid oxidation disorders. Samples can be presented as plasma, urine, and blood spots or urine on filter paper. Sample preparation techniques can include solvent extraction, acid derivitisation and evaporation prior to reconstitution in a suitable medium for injection to MS. Evaporation of butanolic HCl used for derivatisation using blowdown techniques results in corrosion of the system especially pins/needles/jets which can lead to contamination of samples and erroneous results. The EZ-2 with HCI resistance commonly used for these sample preparation stages. One example would be the test for CAT (carnitine acyl carnitine translocase deficiency) in neonates, an inborn error of metabolism. Lack of this enzyme prevents the body from converting fats into energy. Labs do the initial test on Guthrie cards (dried blood spots). The cards are punched and extracted with 200ul of Methanol. The supernatant is placed into microtitre plates and dried. Samples are then derivatised using approx 100ul 3N HCl in anhydrous Butanol and dried again. Finally, samples are re-suspended in Water & acetonitrile for analysis by MS.

miVac concentrators have been used by the Australian Sports Drug Testing Laboratory to improve the testing for synthetic insulin analogues in athletes' blood samples. Using the miVac's ability to control conditions during the concentration step the analyst has more confidence that they are seeing the true blood concentration of potential drugs of abuse in the athlete's sample.


Useful Papers:

  • Evaluation of the EZ-2 for Forensic Toxicology - Dr Eleanor Miller & Dr Simon Elliott, ROAR Forensics, UK. Evaluated the EZ-2 for use concentrating extracts from hair samples to detect alcohol and drugs of abuse metabolites entrained in the samples
  • Evaluation of Evaporative Sample Preparation Techniques for the Extraction of Drugs of Abuse from Urine. Describes the development of a new method for the pre- analytical extraction of drugs of abuse from urine samples pertaining to drug facilitated sexual assault cases. By Forensic Science Ireland (FSI).
  • Better Detection of Insulin Analogues - Great care is required when preparing the samples for analysis, this paper evaluates two possible methods (nitrogen blow down & vacuum concentration) and highlights the pitfalls. Study by Australian Sports Drug Testing Laboratory.

Contact us if you are interested in any of the above papers

DNA & Oligonucleotides

Genevac systems facilitate a wide range of DNA sample preparation and processing activities, ranging from straightforward small volume concentration of DNA pellets prior to PCR, through to high throughput processing of many purified DNA or oligonucleotide samples.  Where a dry sample of DNA is required, concentration to dryness in a centrifugal evaporator is the ideal method, presenting the DNA as a dry film in the tube of plate well.  Freeze drying of DNA samples is not recommended because the resultant DNA powder is easily blown about or moved by electrostatic charges.

DNA Concentration
Concentration of DNA from samples containing water or a mixture of water and alcohols is a simple process, which for most researchers requires the miVac DNA concentrator.  Concentration to remove a few hundred microlitres of alcohol from a DNA pellet takes approximately 10 minutes, or less.   This is one of the simplest applications of a Genevac system – in a miVac DNA concentrator, set the temperature, select the method for alcohols or water, and press start. 

Oligonucleotides & DNA Purification
The manufacture of oligonucleotides commonly has two steps where evaporation is required, evaporation of ammonia solutions following synthesis, and then subsequent evaporation of purified samples.   Protection of oligonucleotides from damage during drying is critical, especially where a tag or label has been attached to the DNA.  Two studies by Genevac users show that this can safely be achieved in a Genevac evaporator: 
  • Comparing Evaporators for Drying Oligonucleotides - Catherine McKeen, Eurogentec SA A Rapid,
  • Safe Technique for Drying Oligonucleotides - Dr Tim Watts, Wellcome Trust Human Genome Centre
Samples containing ammonia solution should be evaporated using Dri-Pure® (500g) to prevent sample loss and cross contamination.  When ammonia solution is subjected to a vacuum the ammonia degasses.  Degassing must be controlled or the resultant foam overflows the tube causing sample loss and cross contamination.  In the same way that you control degassing of a bottle of soda by opening it slowly, gentle application of vacuum is required, this coupled with the high rotors speeds of Dri-Pure keep every sample where it should be.  The latest versions of the EZ-2 come with such a method pre-programmed, and the method is simple to program on the HT-Series.
  
Where samples have been purified by reverse phase HPLC samples are presented in a mixture of water and methanol or acetonitrile.  The methods laid out in the Post Purification Sample Handling section should be followed.  The imbalance tolerance built into Genevac systems gives researchers confidence that eluates at different ends of the gradient will still dry down, and if there is too much imbalance the system will automatically shut down and tell the user.

Preparation of DNA Microarrays
For certain microarraying techniques concentrated samples of DNA are required.  To maintain such a concentration is difficult due to the volatile nature of the buffers holding the DNA, therefore samples should be made up to the required volume immediately prior to analysis.  A study by the Wellcome Trust Centre for Human Genetics in Oxford has shown that when an EZ-2 is used in this process the data quality returned by the system is significantly better, reducing the no call rate by 80% or more.

Environmental Analysis

Monitoring of the environment for substances potentially harmful to health is a core activity not just for government departments but for many organisations who wish to ensure that they are not harming their staff or the environment.  Samples of air, water, soil or plant matter are routinely monitored for the presence of potentially harmful substances – for example, pesticides in river water or the soil. Foodstuffs are similarly monitored.

When testing the water quality of a river, a point sample only provides a snap-shot of the situation, where as monitoring over a period of time will help present a better overall picture and can indicate where there is chronic build-up of a potentially harmful substance(s).  Samples of tissue are often taken from fish living in the river are examined to determine the nature of any chemicals in the water.  Another more recent approach is to use a virtual fish, a semi-permeable membrane device (SPMD) developed by the US Geological Survey.  This is placed in the river for a period of time, and then any organic chemicals are extracted with solvent, concentrated and analysed as they would be from a fish.

Air quality can be monitored similarly by filtering air for a period of time, extracting the filter, and then concentrating and analysing whatever organic molecules may be trapped on the filter.  This is a key activity in industrial environments where PAHs may be a risk factor for workers.

When concentrating samples the EZ-2 (small volumes) and Rocket (large volumes) are ideal tools for concentrating in the analytical laboratory.  Coupled with SampleGenie they enhance analyte recovery, reduce inter-sample variability and free workers to do more productive tasks than monitoring a concentration system. SampleGenie enables large volume samples to be concentrated directly into an autosampler vial, eliminating the need to transfer the sample. 

Useful Papers:
Improving Analysis of Airborne PAHs
Detectionand Quantification of Algal Toxins in Surface Water
Analysis of Glyphosate and its degradation Products in Surface Water


Food & Beverage

Quality and safety testing of food and beverages is an area of growing importance, be that for exporters, importers or government bodies, accurate analytical results are critical.  In common with many other applications, careful sample preparation is critical, especially when the analyte of interest is volatile.

Genevac systems find application in a number of areas, from testing constituents of beverages such as Cognac or gluten levels in Whisky, to pesticide analysis of fruit and vegetables, antibiotic residues in meats or vitamin levels in cereals.  The research and development of Functional Foods where high quality scientific research is carried out also benefits from the speed and quality of a Genevac system.  The EZ-2 or Rocket are commonly used, often in conjunction with SampleGenie™, because this delivers automation of sample transfer and gives unparalleled sample recovery and inter-test reproducibility with very low standard deviations, as the studies from the Laboratoire départemental d'analyses de la Drôme clearly establish.
Concentration technology in Genevac systems has been developed with leading analytical laboratories.  This, with key technologies like Dri-Pure, ensure that samples are concentrated safely and rapidly. SampleGenie™ is a great aid to concentration because the evaporator only evaporates the solvent contained in the large flask, and not the solvent in the vial.  The system detects the end point of evaporation being when the solvent level enters the vial and, once validated, the method will concentrate the sample to the required level.  If a precise volume is required in the vial, then the sample must be over concentrated and then made up to the desired level with pure solvent.

For protein analysis, e.g. for nutritional studies, the EZ-2 with HCI resistance option is ideal.  Protein digests often require digestion with 6N HCl which is highly corrosive to traditional evaporation systems, however, HCl resistant Genevac systems are designed to withstand daily use of concentrated HCl.  

The Rocket has also found an interest application in some of the worlds best restaurants, those that specialise in molecular cuisine, where it is used to concentrate sauces, juices and other natural flavours for the chef to use.  Details of how the Rocket is used can be found in a new book; Modernist Cuisine - the Art and Science of Cooking. The Studio Kitchen blog has some interesting receipe ideas, and some of these are also on Kitchen Theory. 

Useful Papers:

  • An Improved Evaporative Sample Preparation Methodology for Determining Nitrofuran Antibiotic Residues in FoodstuffsKantonales Labor Zurich (KLZH), Zurich, Switzerland 
  • Analysis for pesticides: Method development to increase the recovery of volatile compounds by using EZ-2, a new generation of centrifugal evaporatorEnvironmental Protection Agency of Tuscany (ARPAT), Florence,  Italy        
  • Evaluation of the EZ-2 for Pesticide Analysis Laboratoire départemental d'analyses de la Drôme, Valence, France  
  • Evaluation of SampleGenie™ for Environmental AnalysisLeochimica Laboratories, Zoppola, Italy 
  • Evaluation of the Rocket and SampleGenie for Pesticide AnalysisLaboratoire départemental d'analyses de la Drôme, Valence, France

Proteins & Peptides

Protein concentration is an issue that the biological sciences have been dealing with for years. Many aspects of the study of proteins and exploitation of their therapeutic value requires concentration before and after processing. Each process step can range in scale from setting up a sitting drop of 100nl in microtitre plate based crystallization study to purification of the product from 10,000L fermentation. All of the currently available tools have their draw-backs whether it is from yield loss on membranes to dilution from capture columns.

One of the most commonly used techniques at all scales is membrane concentration. At the small scale this is usually based on concentrators that fit in a centrifuge or small pressure based filers both of which rely on dead-end filtration to push the supernatant through a membrane and retain the protein. The small scale methods used suffer badly from concentration polarization a phenomenon observed in membrane concentrations where the protein concentration at the membrane increases due to the removal of liquid, this is analogous to the cake formed in conventional filtration. In larger applications a cross flow membrane is commonly used where the pressure potential for filtration is provided by a tangential flow to the membrane surface.This cross flow helps to reduce the effect of concentration polarization by sweeping the membrane to clear the retained protein; however, due to constant solvent removal within the system there is still a polarization gradient setup at steady state. When a protein reaches ultra-high concentration at the membrane interface then gel formation is likely this can reduce the activity of the protein and, if the regime for re-suspension is not effective, lead to loss of yield. When we couple this to the non-specific binding effects of most membrane systems it makes this a technique that the industry is using for necessity rather than optimized performance.

One alternative for laboratory scale concentration is to use Centrifugal Vacuum concentration where the solvent is boiled from the supernatant under vacuum so that the temperature of boiling is below the denaturation threshold of the protein. This can dramatically improve both the yield after concentration and also the activity of the protein.This technique would not be used on the large scale but finds a niche in the small scale laboratory work, for example preparation of sample prior to screening against a panel of precipitants to determine the optimum conditions for crystallisation prior to XRD. For hanging and sitting drop experiments concentrations greater than 10 to 20 mg/ml are often required, this is very difficult to achieve in membrane based concentrators due to the limiting factors that have been highlighted above. Where the sample has been purified using reverse phase chromatography (typically peptides), leaving the sample in water and organic solvent, this cannot be concentrated using a membrane because the organic solvents damage the membrane. In a vacuum concentrator the sample remains in a micro-centrifuge tube and the volume remaining can be accurately determined by the time of concentration. One potential limitation of this technique is that the salt and buffer components of the solution will also be concentrated, hence this is ideally suited to be a polishing step that is used after a capture column or membrane concentration step and therefore allows the sample to reach the very high concentrations needed for crystallization. The most commonly used systems for these applications are the miVac range of biological concentrators or the EZ-2.

Peptide Synthesis
Peptide synthesis is normally performed using solid phase supported stub with the sequential addition of protected amino acids. Between each addition step, the protected terminal needs to be deprotected so that the next acid can react and join.  When the desired peptide sequence is complete, it is cleaved from the solid support, usually using a strong acid such as TFA.  The peptide in cleavage cocktail is then dried and purified. Following purification the peptide can be fast lyophilised in a Genevac HT series evaporator or dried using a traditional freeze drier.

Useful Papers:

  • Better Analysis of Insulin Analogues - Dr Catrin Goebel, Australian Sports Drug Testing Lab Examining the effects of sample preparation on data qualityControlled
  • Concentration for MALDI-TOF MS - Steve Knight, GenevacLooking at the effects of sample preparation on MALDI targets in cancer research
Contact us if you are interested in any of the above papers

Using the most advanced proven technology in evaporation science.

EZ-2 series evaporators


Using the most advanced proven technology in evaporation science

The third generation of the EZ-2 Series is the pinnacle of parallel evaporation that draws on the expertise of Genevac engineers and the experiences of many scientists in the laboratory

Racks and Accessories

Brochure

System description

Using the most advanced proven technology in evaporation science, the EZ-2 Series has been designed specifically for solvent removal in many areas of scientific research, whether concentration of samples or complete drying is required.

There are three models in the EZ-2 Series: Standard, Plus & Elite 

• The Standard (EZ-2) is suitable for water and volatile solvents
• The Plus (EZ-2 Plus) is recommended for solvents with boiling points up to 165°C 
• The Elite (EZ-2 Elite) is recommended for solvents with boiling points up to 220°C

Key features: Autostop, Pre-programmed methods, Revolutionary SpeedTrap™ (with autodrain on Elite), DriPure anti-bumping, High boiling point solvents (Elite, )Lyospeed, Fast lyophilisation (Elite)

  • ● Easier to use than a rotary evaporator
  • ● Smart evaporation gives great results every time
  • ● Advanced technology offers total sample protection
  • ● Versatile design accommodates a wide choice of sample types
  • ● All-in-one compact design saves valuable bench space
  • ● Easy maintenance contributes to low cost of ownership

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
031-709 8186

Application Examples

Clinical and Diagnostic Testing

Evaporative sample preparation for forensic and diagnostic clinical tests must be performed scrupulously. Genevac systems are used by clinical biochemists and forensic scientists for a number of testing methodologies, such as vitamin D or K analysis, neonatal screening and forensic toxicology.

Mass Spectrometry (MS) is replacing radio-immunoassay (RIA) in Clinical biochemistry analysis, removing the need to use radioactive reagents. One of the Steroid assays performed by hospital clinical labs is for Vitamin D analysis where there is a need to identify the relative abundance of vitamin D2 and D3. Samples are typically extracted into Hexane, concentrated (e.g. using a miVac) and analysed by MS (avoiding ion suppression problems often seen with RIA).

Clinical and forensic toxicology investigations also utilise MS methodology, typically with solid phase extraction and evaporation as part of the sample preparation process. Blood and urine analysis can be utilised as part of a medico-legal investigation into drug-related deaths and drug-facilitated crimes and in the clinical assessment of alcohol/drug consumption. Hair analysis can also provide evidence over time of drug or alcohol abuse, as part of programme compliance for those participating in drug or alcohol dependency treatment or for workplace or health insurance screening. The EZ-2 has been used to streamline the sample preparation process whilst maintaining sample integrity and improving sensitivity.

Tandem MS is also used to identify metabolic disorders which include aminoacidemias, urea cycle disorders, organic acidurias, and fatty acid oxidation disorders. Samples can be presented as plasma, urine, and blood spots or urine on filter paper. Sample preparation techniques can include solvent extraction, acid derivitisation and evaporation prior to reconstitution in a suitable medium for injection to MS. Evaporation of butanolic HCl used for derivatisation using blowdown techniques results in corrosion of the system especially pins/needles/jets which can lead to contamination of samples and erroneous results. The EZ-2 with HCI resistance commonly used for these sample preparation stages. One example would be the test for CAT (carnitine acyl carnitine translocase deficiency) in neonates, an inborn error of metabolism. Lack of this enzyme prevents the body from converting fats into energy. Labs do the initial test on Guthrie cards (dried blood spots). The cards are punched and extracted with 200ul of Methanol. The supernatant is placed into microtitre plates and dried. Samples are then derivatised using approx 100ul 3N HCl in anhydrous Butanol and dried again. Finally, samples are re-suspended in Water & acetonitrile for analysis by MS.

miVac concentrators have been used by the Australian Sports Drug Testing Laboratory to improve the testing for synthetic insulin analogues in athletes' blood samples. Using the miVac's ability to control conditions during the concentration step the analyst has more confidence that they are seeing the true blood concentration of potential drugs of abuse in the athlete's sample.


Useful Papers:

  • Evaluation of the EZ-2 for Forensic Toxicology - Dr Eleanor Miller & Dr Simon Elliott, ROAR Forensics, UK. Evaluated the EZ-2 for use concentrating extracts from hair samples to detect alcohol and drugs of abuse metabolites entrained in the samples
  • Evaluation of Evaporative Sample Preparation Techniques for the Extraction of Drugs of Abuse from Urine. Describes the development of a new method for the pre- analytical extraction of drugs of abuse from urine samples pertaining to drug facilitated sexual assault cases. By Forensic Science Ireland (FSI).
  • Better Detection of Insulin Analogues - Great care is required when preparing the samples for analysis, this paper evaluates two possible methods (nitrogen blow down & vacuum concentration) and highlights the pitfalls. Study by Australian Sports Drug Testing Laboratory.

Contact us if you are interested in any of the above papers

DNA & Oligonucleotides

Genevac systems facilitate a wide range of DNA sample preparation and processing activities, ranging from straightforward small volume concentration of DNA pellets prior to PCR, through to high throughput processing of many purified DNA or oligonucleotide samples.  Where a dry sample of DNA is required, concentration to dryness in a centrifugal evaporator is the ideal method, presenting the DNA as a dry film in the tube of plate well.  Freeze drying of DNA samples is not recommended because the resultant DNA powder is easily blown about or moved by electrostatic charges.

DNA Concentration
Concentration of DNA from samples containing water or a mixture of water and alcohols is a simple process, which for most researchers requires the miVac DNA concentrator.  Concentration to remove a few hundred microlitres of alcohol from a DNA pellet takes approximately 10 minutes, or less.   This is one of the simplest applications of a Genevac system – in a miVac DNA concentrator, set the temperature, select the method for alcohols or water, and press start. 

Oligonucleotides & DNA Purification
The manufacture of oligonucleotides commonly has two steps where evaporation is required, evaporation of ammonia solutions following synthesis, and then subsequent evaporation of purified samples.   Protection of oligonucleotides from damage during drying is critical, especially where a tag or label has been attached to the DNA.  Two studies by Genevac users show that this can safely be achieved in a Genevac evaporator: 
  • Comparing Evaporators for Drying Oligonucleotides - Catherine McKeen, Eurogentec SA A Rapid,
  • Safe Technique for Drying Oligonucleotides - Dr Tim Watts, Wellcome Trust Human Genome Centre
Samples containing ammonia solution should be evaporated using Dri-Pure® (500g) to prevent sample loss and cross contamination.  When ammonia solution is subjected to a vacuum the ammonia degasses.  Degassing must be controlled or the resultant foam overflows the tube causing sample loss and cross contamination.  In the same way that you control degassing of a bottle of soda by opening it slowly, gentle application of vacuum is required, this coupled with the high rotors speeds of Dri-Pure keep every sample where it should be.  The latest versions of the EZ-2 come with such a method pre-programmed, and the method is simple to program on the HT-Series.
  
Where samples have been purified by reverse phase HPLC samples are presented in a mixture of water and methanol or acetonitrile.  The methods laid out in the Post Purification Sample Handling section should be followed.  The imbalance tolerance built into Genevac systems gives researchers confidence that eluates at different ends of the gradient will still dry down, and if there is too much imbalance the system will automatically shut down and tell the user.

Preparation of DNA Microarrays
For certain microarraying techniques concentrated samples of DNA are required.  To maintain such a concentration is difficult due to the volatile nature of the buffers holding the DNA, therefore samples should be made up to the required volume immediately prior to analysis.  A study by the Wellcome Trust Centre for Human Genetics in Oxford has shown that when an EZ-2 is used in this process the data quality returned by the system is significantly better, reducing the no call rate by 80% or more.

Environmental Analysis

Monitoring of the environment for substances potentially harmful to health is a core activity not just for government departments but for many organisations who wish to ensure that they are not harming their staff or the environment.  Samples of air, water, soil or plant matter are routinely monitored for the presence of potentially harmful substances – for example, pesticides in river water or the soil. Foodstuffs are similarly monitored.

When testing the water quality of a river, a point sample only provides a snap-shot of the situation, where as monitoring over a period of time will help present a better overall picture and can indicate where there is chronic build-up of a potentially harmful substance(s).  Samples of tissue are often taken from fish living in the river are examined to determine the nature of any chemicals in the water.  Another more recent approach is to use a virtual fish, a semi-permeable membrane device (SPMD) developed by the US Geological Survey.  This is placed in the river for a period of time, and then any organic chemicals are extracted with solvent, concentrated and analysed as they would be from a fish.

Air quality can be monitored similarly by filtering air for a period of time, extracting the filter, and then concentrating and analysing whatever organic molecules may be trapped on the filter.  This is a key activity in industrial environments where PAHs may be a risk factor for workers.

When concentrating samples the EZ-2 (small volumes) and Rocket (large volumes) are ideal tools for concentrating in the analytical laboratory.  Coupled with SampleGenie they enhance analyte recovery, reduce inter-sample variability and free workers to do more productive tasks than monitoring a concentration system. SampleGenie enables large volume samples to be concentrated directly into an autosampler vial, eliminating the need to transfer the sample. 

Useful Papers:
Improving Analysis of Airborne PAHs
Detectionand Quantification of Algal Toxins in Surface Water
Analysis of Glyphosate and its degradation Products in Surface Water


Food & Beverage

Quality and safety testing of food and beverages is an area of growing importance, be that for exporters, importers or government bodies, accurate analytical results are critical.  In common with many other applications, careful sample preparation is critical, especially when the analyte of interest is volatile.

Genevac systems find application in a number of areas, from testing constituents of beverages such as Cognac or gluten levels in Whisky, to pesticide analysis of fruit and vegetables, antibiotic residues in meats or vitamin levels in cereals.  The research and development of Functional Foods where high quality scientific research is carried out also benefits from the speed and quality of a Genevac system.  The EZ-2 or Rocket are commonly used, often in conjunction with SampleGenie™, because this delivers automation of sample transfer and gives unparalleled sample recovery and inter-test reproducibility with very low standard deviations, as the studies from the Laboratoire départemental d'analyses de la Drôme clearly establish.
Concentration technology in Genevac systems has been developed with leading analytical laboratories.  This, with key technologies like Dri-Pure, ensure that samples are concentrated safely and rapidly. SampleGenie™ is a great aid to concentration because the evaporator only evaporates the solvent contained in the large flask, and not the solvent in the vial.  The system detects the end point of evaporation being when the solvent level enters the vial and, once validated, the method will concentrate the sample to the required level.  If a precise volume is required in the vial, then the sample must be over concentrated and then made up to the desired level with pure solvent.

For protein analysis, e.g. for nutritional studies, the EZ-2 with HCI resistance option is ideal.  Protein digests often require digestion with 6N HCl which is highly corrosive to traditional evaporation systems, however, HCl resistant Genevac systems are designed to withstand daily use of concentrated HCl.  

The Rocket has also found an interest application in some of the worlds best restaurants, those that specialise in molecular cuisine, where it is used to concentrate sauces, juices and other natural flavours for the chef to use.  Details of how the Rocket is used can be found in a new book; Modernist Cuisine - the Art and Science of Cooking. The Studio Kitchen blog has some interesting receipe ideas, and some of these are also on Kitchen Theory. 

Useful Papers:

  • An Improved Evaporative Sample Preparation Methodology for Determining Nitrofuran Antibiotic Residues in FoodstuffsKantonales Labor Zurich (KLZH), Zurich, Switzerland 
  • Analysis for pesticides: Method development to increase the recovery of volatile compounds by using EZ-2, a new generation of centrifugal evaporatorEnvironmental Protection Agency of Tuscany (ARPAT), Florence,  Italy        
  • Evaluation of the EZ-2 for Pesticide Analysis Laboratoire départemental d'analyses de la Drôme, Valence, France  
  • Evaluation of SampleGenie™ for Environmental AnalysisLeochimica Laboratories, Zoppola, Italy 
  • Evaluation of the Rocket and SampleGenie for Pesticide AnalysisLaboratoire départemental d'analyses de la Drôme, Valence, France

Metabolism & Toxicology Studies

Metabolic and Toxicology studies look at the passage of a drug or chemical into and through the body.  They range from routine safety screening for workers potentially at risk to chemicals through to pharmaceutical drug safety testing and drug prescription compliance and monitoring in the clinical environment.  Genevac systems provide an invaluable tool to researchers in these environments, providing fast, safe evaporative sample preparation free from artefact, contamination and sample loss.Metabolic and Toxicology studies look at the passage of a drug or chemical into and through the body.  They range from routine safety screening for workers potentially at risk to chemicals through to pharmaceutical drug safety testing and drug prescription compliance and monitoring in the clinical environment.  Genevac systems provide an invaluable tool to researchers in these environments, providing fast, safe evaporative sample preparation free from artefact, contamination and sample loss.
 
Applications range from the relatively simple concentration or drying of solvent extracts from tissue samples to qualitatively analyse for the presence of a drug.  Two studies using a miVac Concentrator focus on this work:
  • Better Analasys of Insulin Analogues - Dr Catrin Goebel, Australian Sports Drug Testing Lab 
  • Detection of Diclofenac Residues in Wildlife - Dr Ngaio Richards, Anglia Ruskin University


More complex metabolic studies utilize labelled drug molecules which are fed to subjects.  Samples are taken, extracted and separated via HPLC.  Multiple samples are often collected in scintillant containing microtitre plates, such as the LumaPlate.  These must be carefully dried before analysis via a luminescence reader such as a TopCount.  If the plates are crudely dried the labelled molecule does not come into intimate contact with the scintillant providing a false reading.  The Genevac EZ-2 and HT-4X have been shown to be excellent for this purpose.
 
Customers working with larger volumes have shown that SampleGenie is a useful tool to help automate their sample transfer process without adverse effect on sample recovery.  

Useful Papers: 

  • Evaluation of the EZ-2 for Forensic Toxicology - Dr Eleanor Miller & Dr Simon Elliott, ROAR Forensics, UK.  Evaluated the EZ-2 for use concentrating extracts from hair samples to detect alcohol and drugs of abuse metabolites entrained in the samples. 
  • Increasing Metabolite Recoveries in ADMET Studies - Dr Sophie Mcdougall, Sanofi Aventis. Studies showed that by using a Genevac HT-4 evaporator and Luma plates can increase the sensitivity and hence the recoveries as the extra gravitational force applied helps the sample mixture to dry as a tight pellet at the bottom of the well. 
  • Automation without Robotics - Peter Bennett, Drug Metabolism group at Servier ResearchEvaluation of SampleGenie™ in their laboratory 
  • Advances in Sample-prep for Metabolite Profiling - Dr Flavio Cinato, Nerviano MSTrials with EZ-2 showed that the optimal balance between speed and minimal compound degradation.  
  • Improving Productivity in Toxicology Screening - Rob Darrington, GenevacUsing a modern centrifugal evaporator like Genevac EZ-2 provides automation, user safety, reliability of the analysis and productivity

If you are interested in any of these papers please contatc us.





Natural Products Research

Natural products are researched for many reasons, from biodiversity preservation through flavour or fragrance research, to finding a cure for disease.  Whatever your reason, Genevac systems are used by researchers who are investigating the properties of the natural environment. 

For basic botanical and biological research, the miVac concentrator range are the mainstay for those extracting and purifying DNA or proteins for genome and proteome research.

Genevac systems are widely used by researchers seeking to extract functional molecules from natural sources.  A typical process would be to take a tissue (or micro-organism broth) sample and then perform a primary solvent extraction resulting in a large volume of crude extract.  The crude extract is often concentrated, for which the Rocket Evaporator is ideal, before being fractionated to isolate each component.  Fractionation may use a system such at the Sepbox, one, or two dimensional chromatography which produces many samples from one extract.  These samples need to be evaporated before storage or analysis and may require lyophilisation. Genevac HT series evaporators are used for this purpose. 

Useful Papers:

  • High Throughput Natural Product Discovery - a paper by Stephen Pickrahn, IFF 
  • Rapid Evaporation Processes to Enhance Natural Product Extraction - by Dr John Hester, Principal R&D Engineer – Natural Product Repository Supervisor, University of Mississippi

Parallel Chemistry

Genevac evaporators have been designed from the outset to be robust and withstand the rigors of working in the chemistry laboratory.  Systems were used in the early days for Combinatorial Chemistry where routine use of 100% TFA has given systems a tough field testing. 
Whether working in drug discovery, flavour or fragrence research, liquid chrystal synthesis or academic reaserch, Genevac systems are the ideal tool for use when performing parallel chemistry.  When working with a single sample then a rotary evaporator is ideal, whereas, when you have multiple samples this would be a bottleneck.  Spending time just watching a rotary evaporator is not very efficient!
  • The main uses for Genevac systems are:
  • ● Solvent exchange – removal of reaction solvents between stages
  • ● Re-salt formation – removal of strong acids of bases when changing salt forms.  Genevac have a special option for HCl resistance which enables the use of concentrated HCl – ideal for this purpose.
  • ● Post Purification Handling – removing purification solvents following normal phase, reverse phase or chiral separation. Genevac also offer SampleGenie to enable a large volume to be dried directly into a small storage which eliminates the need to reformat samples from a flask into a vial.  Reverse phase HPLC fractions may also be fast lyophilised in some Genevac systems.
  • ● Compound logistics - transfer to and management of samples in storage conditions and systems, this is often done using DMSO, a notoriously difficult to evaporate high boiling point solvent.
  • Depending on the number and nature of samples then one of several Genevac systems may be suitable – the EZ-2 and HT series are designed for working with smaller samples in plates, tubes and vials.  When working with large volumes in flasks – then the Rocket evaporator is the most suitable, taking up to 6 flasks.

Useful Papers:

  • Reactions involving HCl and their Evaporation - Dr Induka Abeysena, Genevac 
  • Development of an Evaporator for High Throughput Chemistry- Dr Tom Smith, GSK 
  • Surviving TFA - Application Note Parallel Dry-Loading of Synthesis Samples onto Silica for Analysis- Dr Nigel Vicker, Bath University Pharmacy Department  
  • Library Maintenance for the Smaller Pharma Company - poster by Dr Sally Hampton, Ferring Pharmaceuticals 
  • How to Evaporate High Boiling Point Solvents - Application Note

Contact us if you are interested in any of the above papers

Proteins & Peptides

Protein concentration is an issue that the biological sciences have been dealing with for years. Many aspects of the study of proteins and exploitation of their therapeutic value requires concentration before and after processing. Each process step can range in scale from setting up a sitting drop of 100nl in microtitre plate based crystallization study to purification of the product from 10,000L fermentation. All of the currently available tools have their draw-backs whether it is from yield loss on membranes to dilution from capture columns.

One of the most commonly used techniques at all scales is membrane concentration. At the small scale this is usually based on concentrators that fit in a centrifuge or small pressure based filers both of which rely on dead-end filtration to push the supernatant through a membrane and retain the protein. The small scale methods used suffer badly from concentration polarization a phenomenon observed in membrane concentrations where the protein concentration at the membrane increases due to the removal of liquid, this is analogous to the cake formed in conventional filtration. In larger applications a cross flow membrane is commonly used where the pressure potential for filtration is provided by a tangential flow to the membrane surface.This cross flow helps to reduce the effect of concentration polarization by sweeping the membrane to clear the retained protein; however, due to constant solvent removal within the system there is still a polarization gradient setup at steady state. When a protein reaches ultra-high concentration at the membrane interface then gel formation is likely this can reduce the activity of the protein and, if the regime for re-suspension is not effective, lead to loss of yield. When we couple this to the non-specific binding effects of most membrane systems it makes this a technique that the industry is using for necessity rather than optimized performance.

One alternative for laboratory scale concentration is to use Centrifugal Vacuum concentration where the solvent is boiled from the supernatant under vacuum so that the temperature of boiling is below the denaturation threshold of the protein. This can dramatically improve both the yield after concentration and also the activity of the protein.This technique would not be used on the large scale but finds a niche in the small scale laboratory work, for example preparation of sample prior to screening against a panel of precipitants to determine the optimum conditions for crystallisation prior to XRD. For hanging and sitting drop experiments concentrations greater than 10 to 20 mg/ml are often required, this is very difficult to achieve in membrane based concentrators due to the limiting factors that have been highlighted above. Where the sample has been purified using reverse phase chromatography (typically peptides), leaving the sample in water and organic solvent, this cannot be concentrated using a membrane because the organic solvents damage the membrane. In a vacuum concentrator the sample remains in a micro-centrifuge tube and the volume remaining can be accurately determined by the time of concentration. One potential limitation of this technique is that the salt and buffer components of the solution will also be concentrated, hence this is ideally suited to be a polishing step that is used after a capture column or membrane concentration step and therefore allows the sample to reach the very high concentrations needed for crystallization. The most commonly used systems for these applications are the miVac range of biological concentrators or the EZ-2.

Peptide Synthesis
Peptide synthesis is normally performed using solid phase supported stub with the sequential addition of protected amino acids. Between each addition step, the protected terminal needs to be deprotected so that the next acid can react and join.  When the desired peptide sequence is complete, it is cleaved from the solid support, usually using a strong acid such as TFA.  The peptide in cleavage cocktail is then dried and purified. Following purification the peptide can be fast lyophilised in a Genevac HT series evaporator or dried using a traditional freeze drier.

Useful Papers:

  • Better Analysis of Insulin Analogues - Dr Catrin Goebel, Australian Sports Drug Testing Lab Examining the effects of sample preparation on data qualityControlled
  • Concentration for MALDI-TOF MS - Steve Knight, GenevacLooking at the effects of sample preparation on MALDI targets in cancer research
Contact us if you are interested in any of the above papers

Post Purification Sample Handling

Depending on the process, and therefore the nature of the samples, then Genevac provides a number of solutions to facilitate post purification sample handling, including reformatting.

  • Normal Phase HPLC, SFC or Flash chromatography – removal of organic solvents is simple in a Genevac evaporator. Evaporation of Solvent Mixtures is safe when using Dri-Pure which eliminates the effects of bumping.
  • Reverse Phase HPLC– evaporation of water and acetonitrile (or methanol) is the principal application of many Genevac systems.  A multiple stage method has been developed to remove the organic solvent without freezing the water, remove the water, and then dry any remaining stubborn solvent.  Alternatively, the LyoSpeed fast lyopilisation method can be used in Genevac HT series evaporators.  Recent advances in condenser technology, in particular the high power -75°C cold trap available with the HT-8 or HT-12, have lead to significant advances in lyophilisation success rates.

Post Purification Handling following removal of purification solvents the sample typically needs to be reformatting from the fraction tubes or flask, into a small vial for compound storage. Genevac developed SampleGenie to enable a large volume to be dried (or lyophilised) directly into a small storage vial which eliminates the need to reformat samples from a flask or multiple tubes into a vial.

Depending on the number and nature of samples then one of several Genevac systems may be suitable – the EZ-2 and HT series are designed for working with smaller samples in plates, tubes and vials.  When working with large volumes in flasks – then the Rocket evaporator is the most suitable, taking up to 6 flasks.

Useful Papers: 
  • Drying HPLC Fractions - applications note 
  • Fast Lyophilisation for HPLC Fractions - Dr Induka Abeysena & Rob Darrington  
  • Enhanced LyoSpeed™ with a -75°C Condenser - Rob Darrington 
  • Integration for High Throughput HPLC Purification - Paul de Fraine, Syngenta & Dr Mike Jones 
  • Drying Solvent Mixtures - applications note 
  • Evaluation of the Rocket Evaporation System - paper by Christine Prosser et al., UCB
  • Automation of reverse phase HPLC fraction pooling, evaporation, and reformatting
Contact us if you are interested in any of the above papers

Oils, Fuels and Fats

Working with oils, fuels and fats can be difficult.  Whether extracting & recovering additives, evaluating lipid content of foods, characterising reclaimed cooking oils before reprocessing or conversion to biofuels, or investigating drillling test samples - oils can be difficult to dry in turn leading to problems with the analytical instrumentation.   

Extraction and separation techniques, be they basic dialysis, Dionex ASE or Counter Current Chromatography, all normally present the sample in a solution of organic solvent.  The sample typcially must be dried or concentrated before analysis.  Drying all the solvent can be difficult without using very high temperatures or other harsh regimens which may lead to loss of more volatile fractions within the oil.  For many such applications, researchers have found that the EZ-2 and the Rocket Evaporator have delivered significant enhancement to their recovery and results.  When working with larger solvent volumes, additional benefit is gained by way of  productivity savings when used with SampleGenie which enables large volumes to be concentrated or dried directly into a small vial.

The ultimate in solvent removal technology.

      

HT-6 and HT-12 evaporators


The ultimate in solvent removal technology

Genevac HT series evaporators are the ideal solution for parallel evaporation bottlenecks in high throughput and production laboratories having high performance and high sample capacities.

Racks and accessories

Brochure

System description

The all new Series 3 HT evaporator range from Genevac represents the ultimate in solvent removal technology. Incorporating new high performance vacuum pump, the latest touchscreen technology and a sleek ergonomic design makes optimising your evaporation processes effortless.

New intuitive touchscreen controls enhances monitoring and review of the whole evaporation process. For popular solvent removal protocols pre-set "Press & Go" methods makes operation easy and productive even for occasional users. Simplified manual and automatic programming means even the most complex multi-stage evaporation methods can be set-up and run quickly and easily.
Peace of mind comes as standard with patented temperature control and bumping prevention built in.
  • ● High throughput evaporation
  • ● High speed evaporation
  • ● Fast lyophilisation capability
  • ● New intuitive touchscreen control system for ultimate ease of use
  • ● Integrated condenser meaning a smaller system footprint
  • ● Front opening for easy access
  • ● DriPure anti-bumping

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
031-709 8186

Application Examples

Parallel Chemistry

Genevac evaporators have been designed from the outset to be robust and withstand the rigors of working in the chemistry laboratory.  Systems were used in the early days for Combinatorial Chemistry where routine use of 100% TFA has given systems a tough field testing. 
Whether working in drug discovery, flavour or fragrence research, liquid chrystal synthesis or academic reaserch, Genevac systems are the ideal tool for use when performing parallel chemistry.  When working with a single sample then a rotary evaporator is ideal, whereas, when you have multiple samples this would be a bottleneck.  Spending time just watching a rotary evaporator is not very efficient!
  • The main uses for Genevac systems are:
  • ● Solvent exchange – removal of reaction solvents between stages
  • ● Re-salt formation – removal of strong acids of bases when changing salt forms.  Genevac have a special option for HCl resistance which enables the use of concentrated HCl – ideal for this purpose.
  • ● Post Purification Handling – removing purification solvents following normal phase, reverse phase or chiral separation. Genevac also offer SampleGenie to enable a large volume to be dried directly into a small storage which eliminates the need to reformat samples from a flask into a vial.  Reverse phase HPLC fractions may also be fast lyophilised in some Genevac systems.
  • ● Compound logistics - transfer to and management of samples in storage conditions and systems, this is often done using DMSO, a notoriously difficult to evaporate high boiling point solvent.
  • Depending on the number and nature of samples then one of several Genevac systems may be suitable – the EZ-2 and HT series are designed for working with smaller samples in plates, tubes and vials.  When working with large volumes in flasks – then the Rocket evaporator is the most suitable, taking up to 6 flasks.

Useful Papers:

  • Reactions involving HCl and their Evaporation - Dr Induka Abeysena, Genevac 
  • Development of an Evaporator for High Throughput Chemistry- Dr Tom Smith, GSK 
  • Surviving TFA - Application Note Parallel Dry-Loading of Synthesis Samples onto Silica for Analysis- Dr Nigel Vicker, Bath University Pharmacy Department  
  • Library Maintenance for the Smaller Pharma Company - poster by Dr Sally Hampton, Ferring Pharmaceuticals 
  • How to Evaporate High Boiling Point Solvents - Application Note

Contact us if you are interested in any of the above papers

Post Purification Sample Handling

Depending on the process, and therefore the nature of the samples, then Genevac provides a number of solutions to facilitate post purification sample handling, including reformatting.

  • Normal Phase HPLC, SFC or Flash chromatography – removal of organic solvents is simple in a Genevac evaporator. Evaporation of Solvent Mixtures is safe when using Dri-Pure which eliminates the effects of bumping.
  • Reverse Phase HPLC– evaporation of water and acetonitrile (or methanol) is the principal application of many Genevac systems.  A multiple stage method has been developed to remove the organic solvent without freezing the water, remove the water, and then dry any remaining stubborn solvent.  Alternatively, the LyoSpeed fast lyopilisation method can be used in Genevac HT series evaporators.  Recent advances in condenser technology, in particular the high power -75°C cold trap available with the HT-8 or HT-12, have lead to significant advances in lyophilisation success rates.

Post Purification Handling following removal of purification solvents the sample typically needs to be reformatting from the fraction tubes or flask, into a small vial for compound storage. Genevac developed SampleGenie to enable a large volume to be dried (or lyophilised) directly into a small storage vial which eliminates the need to reformat samples from a flask or multiple tubes into a vial.

Depending on the number and nature of samples then one of several Genevac systems may be suitable – the EZ-2 and HT series are designed for working with smaller samples in plates, tubes and vials.  When working with large volumes in flasks – then the Rocket evaporator is the most suitable, taking up to 6 flasks.

Useful Papers: 
  • Drying HPLC Fractions - applications note 
  • Fast Lyophilisation for HPLC Fractions - Dr Induka Abeysena & Rob Darrington  
  • Enhanced LyoSpeed™ with a -75°C Condenser - Rob Darrington 
  • Integration for High Throughput HPLC Purification - Paul de Fraine, Syngenta & Dr Mike Jones 
  • Drying Solvent Mixtures - applications note 
  • Evaluation of the Rocket Evaporation System - paper by Christine Prosser et al., UCB
  • Automation of reverse phase HPLC fraction pooling, evaporation, and reformatting
Contact us if you are interested in any of the above papers

Controlled Crystallisation

Crystallisation is a critical process in the development of many compounds. It can form part of the purifications or separation stage and can influence yield purity and particle size. In API development particularly the identification of different crystalline polymorphs and confirmation of stable & metastable forms is very important.

Chemists achieve crystalisation by many varied methods. Variation in temperature, solvent, head pressure, seeding method and evaporation rate can all have effects on the crystallisation process, influencing the form, shape and size of crystals formed. However for many methods used there is little control and often no reproducibility of these parameters. Such studies can also be time consuming and difficult to conduct

EXALT is a unique toolkit developed by Genevac and researchers in the field to assist with evaporative crystallisation studies in a number ways. By enabling a wide range of solvents to be evaporated at the same time and at the same slow rate, the EXALT system can deliver crystalline forms of a compound in a controlled and reproducible manner. It gives control to the operator by removing a number variables which are often present in current methods for crystallisation studies.

EXALT has been shown to be a useful tool for confirmation of stable forms, to create crystals from amorphous forms, to screen solvents for crystal production including production of seed crystals, and in co-crystal screening.

eXalt Brochure

Environmental Analysis

Monitoring of the environment for substances potentially harmful to health is a core activity not just for government departments but for many organisations who wish to ensure that they are not harming their staff or the environment.  Samples of air, water, soil or plant matter are routinely monitored for the presence of potentially harmful substances – for example, pesticides in river water or the soil. Foodstuffs are similarly monitored.

When testing the water quality of a river, a point sample only provides a snap-shot of the situation, where as monitoring over a period of time will help present a better overall picture and can indicate where there is chronic build-up of a potentially harmful substance(s).  Samples of tissue are often taken from fish living in the river are examined to determine the nature of any chemicals in the water.  Another more recent approach is to use a virtual fish, a semi-permeable membrane device (SPMD) developed by the US Geological Survey.  This is placed in the river for a period of time, and then any organic chemicals are extracted with solvent, concentrated and analysed as they would be from a fish.

Air quality can be monitored similarly by filtering air for a period of time, extracting the filter, and then concentrating and analysing whatever organic molecules may be trapped on the filter.  This is a key activity in industrial environments where PAHs may be a risk factor for workers.

When concentrating samples the EZ-2 (small volumes) and Rocket (large volumes) are ideal tools for concentrating in the analytical laboratory.  Coupled with SampleGenie they enhance analyte recovery, reduce inter-sample variability and free workers to do more productive tasks than monitoring a concentration system. SampleGenie enables large volume samples to be concentrated directly into an autosampler vial, eliminating the need to transfer the sample. 

Useful Papers:
Improving Analysis of Airborne PAHs
Detectionand Quantification of Algal Toxins in Surface Water
Analysis of Glyphosate and its degradation Products in Surface Water


Metabolism & Toxicology Studies

Metabolic and Toxicology studies look at the passage of a drug or chemical into and through the body.  They range from routine safety screening for workers potentially at risk to chemicals through to pharmaceutical drug safety testing and drug prescription compliance and monitoring in the clinical environment.  Genevac systems provide an invaluable tool to researchers in these environments, providing fast, safe evaporative sample preparation free from artefact, contamination and sample loss.Metabolic and Toxicology studies look at the passage of a drug or chemical into and through the body.  They range from routine safety screening for workers potentially at risk to chemicals through to pharmaceutical drug safety testing and drug prescription compliance and monitoring in the clinical environment.  Genevac systems provide an invaluable tool to researchers in these environments, providing fast, safe evaporative sample preparation free from artefact, contamination and sample loss.
 
Applications range from the relatively simple concentration or drying of solvent extracts from tissue samples to qualitatively analyse for the presence of a drug.  Two studies using a miVac Concentrator focus on this work:
  • Better Analasys of Insulin Analogues - Dr Catrin Goebel, Australian Sports Drug Testing Lab 
  • Detection of Diclofenac Residues in Wildlife - Dr Ngaio Richards, Anglia Ruskin University


More complex metabolic studies utilize labelled drug molecules which are fed to subjects.  Samples are taken, extracted and separated via HPLC.  Multiple samples are often collected in scintillant containing microtitre plates, such as the LumaPlate.  These must be carefully dried before analysis via a luminescence reader such as a TopCount.  If the plates are crudely dried the labelled molecule does not come into intimate contact with the scintillant providing a false reading.  The Genevac EZ-2 and HT-4X have been shown to be excellent for this purpose.
 
Customers working with larger volumes have shown that SampleGenie is a useful tool to help automate their sample transfer process without adverse effect on sample recovery.  

Useful Papers: 

  • Evaluation of the EZ-2 for Forensic Toxicology - Dr Eleanor Miller & Dr Simon Elliott, ROAR Forensics, UK.  Evaluated the EZ-2 for use concentrating extracts from hair samples to detect alcohol and drugs of abuse metabolites entrained in the samples. 
  • Increasing Metabolite Recoveries in ADMET Studies - Dr Sophie Mcdougall, Sanofi Aventis. Studies showed that by using a Genevac HT-4 evaporator and Luma plates can increase the sensitivity and hence the recoveries as the extra gravitational force applied helps the sample mixture to dry as a tight pellet at the bottom of the well. 
  • Automation without Robotics - Peter Bennett, Drug Metabolism group at Servier ResearchEvaluation of SampleGenie™ in their laboratory 
  • Advances in Sample-prep for Metabolite Profiling - Dr Flavio Cinato, Nerviano MSTrials with EZ-2 showed that the optimal balance between speed and minimal compound degradation.  
  • Improving Productivity in Toxicology Screening - Rob Darrington, GenevacUsing a modern centrifugal evaporator like Genevac EZ-2 provides automation, user safety, reliability of the analysis and productivity

If you are interested in any of these papers please contatc us.





Natural Products Research

Natural products are researched for many reasons, from biodiversity preservation through flavour or fragrance research, to finding a cure for disease.  Whatever your reason, Genevac systems are used by researchers who are investigating the properties of the natural environment. 

For basic botanical and biological research, the miVac concentrator range are the mainstay for those extracting and purifying DNA or proteins for genome and proteome research.

Genevac systems are widely used by researchers seeking to extract functional molecules from natural sources.  A typical process would be to take a tissue (or micro-organism broth) sample and then perform a primary solvent extraction resulting in a large volume of crude extract.  The crude extract is often concentrated, for which the Rocket Evaporator is ideal, before being fractionated to isolate each component.  Fractionation may use a system such at the Sepbox, one, or two dimensional chromatography which produces many samples from one extract.  These samples need to be evaporated before storage or analysis and may require lyophilisation. Genevac HT series evaporators are used for this purpose. 

Useful Papers:

  • High Throughput Natural Product Discovery - a paper by Stephen Pickrahn, IFF 
  • Rapid Evaporation Processes to Enhance Natural Product Extraction - by Dr John Hester, Principal R&D Engineer – Natural Product Repository Supervisor, University of Mississippi

For drying or concentrating very large volumes.

Rocket 4D Synergy


For drying or concentrating very large volumes

Designed primarily to evaporate large sample volumes (of 5 litres and above), with autofeed if required.

Brochure

System description

Rocket 4D Synergy is a fully automated system for drying or concentrating very large volumes with no user intervention and in complete confidence. Designed primarily to evaporate large sample volumes (of 5 litres and above), with autofeed if required, the Rocket 4D Synergy can also be fitted with a removable flask rotor to accommodate the full range of Rocket Sample handling solutions such as flasks, tubes and SampleGenie.

Now you don't need to choose between flask, batch or feed evaporation. The Rocket Synergy evaporator will accommodate your process, whatever the volume. Each is capable of replacing several rotary evaporators and saving valuable bench space.

Key features: High speed evaporation, High volume evaporation - Sample volumes from 50ml to 100 liters, Autofeed, Autostop, Unique Rocket low pressure steam heat transfer technology, DriPure anti bumping, Removable interchangeable rotors, Autodraining condenser, Strobe to view evaporation progress
  • ● Easy to use – simple controls and intelligent software
  • ● Fully automated from start to finish
  • ● Space saving –one Rocket replaces several rotary evaporators
  • ● Perfect results – no monitoring or intervention required for excellent sample recovery
  • ● Environmentally friendly cold traps and advanced methodology provide very high solvent recovery

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
031-709 8186

Applications

Rocket 4D Synergy is a fully automated system for drying or concentrating very large volumes with no user intervention and in complete confidence –no bumping, foaming or sample loss. Simply load your sample, select the correct method,press start, and walk away –the system will do the rest.The Rocket 4D Synergy can also befitted with a removable flask rotor to accommodate the full range of Rocket Sample handling solutions such as flasks, tubes and SampleGenie.

Options

Space saving –one Rocket replaces several rotary evaporators.

Rocket Synergy


Space saving –one Rocket replaces several rotary evaporators

Designed to dry or concentrate up to six flasks, each containing a maximum of 450ml of solvent, or 18 ASE® vials, with no user intervention or attention

Options for Rocket Synergy

Brochure

System description

The removable flask rotor may also be replaced with a 5 litre stainless steel vessel for batch processing
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Rocket controls are very easy to use. Load your samples, select the correct method,press start and walk away. The evaporator is equipped with high performance features that prevent foaming, bumping and cross contamination. A built-in cold trap provides very high levels of solvent recovery, even with volatile organic solvents. Auto-draining, under the control of the Rocket, ensures optimal solvent recovery is maintained under all conditions.

Now you don't need to choose between flask or batch evaporation. The Rocket Synergy evaporator will accommodate your process, whatever the volume. Each is capable of replacing several rotary evaporators and saving valuable bench space.

Key features: High speed evaporation, High speed evaporation, Autostop, Unique Rocket low pressure steam heat transfer technology, DriPure anti bumping, Sample volumes from 50ml to 5 litres, Removable interchangeable rotors, Autodraining condenser, Strobe to view evaporation progress
  • ● Easy to use – simple controls and intelligent software
  • ● Fully automated from start to finish
  • ● Space saving –one Rocket replaces several rotary evaporators
  • ● Perfect results – no monitoring or intervention required for excellent sample recovery
  • ● Environmentally friendly cold traps and advanced methodology provide very high solvent recovery

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
031-709 8186

Application Examples

Parallel Chemistry

Genevac evaporators have been designed from the outset to be robust and withstand the rigors of working in the chemistry laboratory.  Systems were used in the early days for Combinatorial Chemistry where routine use of 100% TFA has given systems a tough field testing. 
Whether working in drug discovery, flavour or fragrence research, liquid chrystal synthesis or academic reaserch, Genevac systems are the ideal tool for use when performing parallel chemistry.  When working with a single sample then a rotary evaporator is ideal, whereas, when you have multiple samples this would be a bottleneck.  Spending time just watching a rotary evaporator is not very efficient!
  • The main uses for Genevac systems are:
  • ● Solvent exchange – removal of reaction solvents between stages
  • ● Re-salt formation – removal of strong acids of bases when changing salt forms.  Genevac have a special option for HCl resistance which enables the use of concentrated HCl – ideal for this purpose.
  • ● Post Purification Handling – removing purification solvents following normal phase, reverse phase or chiral separation. Genevac also offer SampleGenie to enable a large volume to be dried directly into a small storage which eliminates the need to reformat samples from a flask into a vial.  Reverse phase HPLC fractions may also be fast lyophilised in some Genevac systems.
  • ● Compound logistics - transfer to and management of samples in storage conditions and systems, this is often done using DMSO, a notoriously difficult to evaporate high boiling point solvent.
  • Depending on the number and nature of samples then one of several Genevac systems may be suitable – the EZ-2 and HT series are designed for working with smaller samples in plates, tubes and vials.  When working with large volumes in flasks – then the Rocket evaporator is the most suitable, taking up to 6 flasks.

Useful Papers:

  • Reactions involving HCl and their Evaporation - Dr Induka Abeysena, Genevac 
  • Development of an Evaporator for High Throughput Chemistry- Dr Tom Smith, GSK 
  • Surviving TFA - Application Note Parallel Dry-Loading of Synthesis Samples onto Silica for Analysis- Dr Nigel Vicker, Bath University Pharmacy Department  
  • Library Maintenance for the Smaller Pharma Company - poster by Dr Sally Hampton, Ferring Pharmaceuticals 
  • How to Evaporate High Boiling Point Solvents - Application Note

Contact us if you are interested in any of the above papers

Food & Beverage

Quality and safety testing of food and beverages is an area of growing importance, be that for exporters, importers or government bodies, accurate analytical results are critical.  In common with many other applications, careful sample preparation is critical, especially when the analyte of interest is volatile.

Genevac systems find application in a number of areas, from testing constituents of beverages such as Cognac or gluten levels in Whisky, to pesticide analysis of fruit and vegetables, antibiotic residues in meats or vitamin levels in cereals.  The research and development of Functional Foods where high quality scientific research is carried out also benefits from the speed and quality of a Genevac system.  The EZ-2 or Rocket are commonly used, often in conjunction with SampleGenie™, because this delivers automation of sample transfer and gives unparalleled sample recovery and inter-test reproducibility with very low standard deviations, as the studies from the Laboratoire départemental d'analyses de la Drôme clearly establish.
Concentration technology in Genevac systems has been developed with leading analytical laboratories.  This, with key technologies like Dri-Pure, ensure that samples are concentrated safely and rapidly. SampleGenie™ is a great aid to concentration because the evaporator only evaporates the solvent contained in the large flask, and not the solvent in the vial.  The system detects the end point of evaporation being when the solvent level enters the vial and, once validated, the method will concentrate the sample to the required level.  If a precise volume is required in the vial, then the sample must be over concentrated and then made up to the desired level with pure solvent.

For protein analysis, e.g. for nutritional studies, the EZ-2 with HCI resistance option is ideal.  Protein digests often require digestion with 6N HCl which is highly corrosive to traditional evaporation systems, however, HCl resistant Genevac systems are designed to withstand daily use of concentrated HCl.  

The Rocket has also found an interest application in some of the worlds best restaurants, those that specialise in molecular cuisine, where it is used to concentrate sauces, juices and other natural flavours for the chef to use.  Details of how the Rocket is used can be found in a new book; Modernist Cuisine - the Art and Science of Cooking. The Studio Kitchen blog has some interesting receipe ideas, and some of these are also on Kitchen Theory. 

Useful Papers:

  • An Improved Evaporative Sample Preparation Methodology for Determining Nitrofuran Antibiotic Residues in FoodstuffsKantonales Labor Zurich (KLZH), Zurich, Switzerland 
  • Analysis for pesticides: Method development to increase the recovery of volatile compounds by using EZ-2, a new generation of centrifugal evaporatorEnvironmental Protection Agency of Tuscany (ARPAT), Florence,  Italy        
  • Evaluation of the EZ-2 for Pesticide Analysis Laboratoire départemental d'analyses de la Drôme, Valence, France  
  • Evaluation of SampleGenie™ for Environmental AnalysisLeochimica Laboratories, Zoppola, Italy 
  • Evaluation of the Rocket and SampleGenie for Pesticide AnalysisLaboratoire départemental d'analyses de la Drôme, Valence, France

Natural Products Research

Natural products are researched for many reasons, from biodiversity preservation through flavour or fragrance research, to finding a cure for disease.  Whatever your reason, Genevac systems are used by researchers who are investigating the properties of the natural environment. 

For basic botanical and biological research, the miVac concentrator range are the mainstay for those extracting and purifying DNA or proteins for genome and proteome research.

Genevac systems are widely used by researchers seeking to extract functional molecules from natural sources.  A typical process would be to take a tissue (or micro-organism broth) sample and then perform a primary solvent extraction resulting in a large volume of crude extract.  The crude extract is often concentrated, for which the Rocket Evaporator is ideal, before being fractionated to isolate each component.  Fractionation may use a system such at the Sepbox, one, or two dimensional chromatography which produces many samples from one extract.  These samples need to be evaporated before storage or analysis and may require lyophilisation. Genevac HT series evaporators are used for this purpose. 

Useful Papers:

  • High Throughput Natural Product Discovery - a paper by Stephen Pickrahn, IFF 
  • Rapid Evaporation Processes to Enhance Natural Product Extraction - by Dr John Hester, Principal R&D Engineer – Natural Product Repository Supervisor, University of Mississippi

Post Purification Sample Handling

Depending on the process, and therefore the nature of the samples, then Genevac provides a number of solutions to facilitate post purification sample handling, including reformatting.

  • Normal Phase HPLC, SFC or Flash chromatography – removal of organic solvents is simple in a Genevac evaporator. Evaporation of Solvent Mixtures is safe when using Dri-Pure which eliminates the effects of bumping.
  • Reverse Phase HPLC– evaporation of water and acetonitrile (or methanol) is the principal application of many Genevac systems.  A multiple stage method has been developed to remove the organic solvent without freezing the water, remove the water, and then dry any remaining stubborn solvent.  Alternatively, the LyoSpeed fast lyopilisation method can be used in Genevac HT series evaporators.  Recent advances in condenser technology, in particular the high power -75°C cold trap available with the HT-8 or HT-12, have lead to significant advances in lyophilisation success rates.

Post Purification Handling following removal of purification solvents the sample typically needs to be reformatting from the fraction tubes or flask, into a small vial for compound storage. Genevac developed SampleGenie to enable a large volume to be dried (or lyophilised) directly into a small storage vial which eliminates the need to reformat samples from a flask or multiple tubes into a vial.

Depending on the number and nature of samples then one of several Genevac systems may be suitable – the EZ-2 and HT series are designed for working with smaller samples in plates, tubes and vials.  When working with large volumes in flasks – then the Rocket evaporator is the most suitable, taking up to 6 flasks.

Useful Papers: 
  • Drying HPLC Fractions - applications note 
  • Fast Lyophilisation for HPLC Fractions - Dr Induka Abeysena & Rob Darrington  
  • Enhanced LyoSpeed™ with a -75°C Condenser - Rob Darrington 
  • Integration for High Throughput HPLC Purification - Paul de Fraine, Syngenta & Dr Mike Jones 
  • Drying Solvent Mixtures - applications note 
  • Evaluation of the Rocket Evaporation System - paper by Christine Prosser et al., UCB
  • Automation of reverse phase HPLC fraction pooling, evaporation, and reformatting
Contact us if you are interested in any of the above papers

Oils, Fuels and Fats

Working with oils, fuels and fats can be difficult.  Whether extracting & recovering additives, evaluating lipid content of foods, characterising reclaimed cooking oils before reprocessing or conversion to biofuels, or investigating drillling test samples - oils can be difficult to dry in turn leading to problems with the analytical instrumentation.   

Extraction and separation techniques, be they basic dialysis, Dionex ASE or Counter Current Chromatography, all normally present the sample in a solution of organic solvent.  The sample typcially must be dried or concentrated before analysis.  Drying all the solvent can be difficult without using very high temperatures or other harsh regimens which may lead to loss of more volatile fractions within the oil.  For many such applications, researchers have found that the EZ-2 and the Rocket Evaporator have delivered significant enhancement to their recovery and results.  When working with larger solvent volumes, additional benefit is gained by way of  productivity savings when used with SampleGenie which enables large volumes to be concentrated or dried directly into a small vial.

Labtech Nitrogen evaporators or nitrogen blowdown concentrators

Nitrogen Concentrators


Labtech’s range covers systems from 6 tubes up to 54

Concentrators ( nitrogen evaporators or nitrogen blowdown concentrators) are a valid alternative to rotary evaporation mainly in case of small volumes (under 50 mL) and a large quantity of samples.

Broschure

Safety, productivity, flexibility and reliability

Concentrators accelerate the evaporation by decreasing the partial vapor pressure of the solvent just above the surface of the liquid. Blowdown evaporators use a jet of gas to continually force off the layer of air that is saturated with vapor. By immediately removing molecules that enter the vapor state, the molecules do not have a chance to return to the liquid, which increases the evaporation rate. As said blowdown technique is very useful for small sample volumes because with larger samples nitrogen blowdown will take longer and semi-volatile compounds may be lost. Since the samples are immersed in a water bath, with programmable temperatures up to 100°C, the evaporation is further accelerated. LabTech offers 4 models, 3 of them automated and one manual, hosting different tube sizes to satisfy multiple needs.

  • ● MultiVap 54 - automatic

  • ● MultiVap 8 - automatic

  • ● MultiVap 10 - automatic

  • ● MultiVap 6 - manual

Product Specialist

Mikael Alfredsson

"Our expert in Digestion, Extraction, Syntesis and Evaporation with more than 20 years experience in the field of sales and service. Contact me for if you have questions or need a quotation"

This email address is being protected from spambots. You need JavaScript enabled to view it.
031-709 8186

MultiVap 54

The MultiVap 54 is the latest multi-channel and multifunctional parallel instrument designed by LabTech, including all accessories and functions to ensure the highest standard of effi ciency, speed, reliability, throughput and safety of any concentration process. Nitrogen sweeping, needle level adjustment and visible concentration process are only few of the advantages offered by the MultiVap 54.

Video MultiVap 54

MultiVap 10

The new LabTech MultiVap 10 automatic parallel system increases the capacity by 25% in comparison to the MultiVap 8 boosting the lab throughput. The price/performance ratio has further increased. With this system it is possible to evaporate to solid state or concentrate into a fixed end-point volume. A compact solution compared to conventional rotary evaporators, with the possibility to be used outside the fume hood thanks to the integrated vapor evacuation system.

MultiVap 8

The LabTech MultiVap 8 system increases the capacity and sample throughput in laboratories and maintains quality and low cost. With this system it is possible to evaporate to solid state or concentrate into a fixed end-point volume. A compact solution compared to conventional rotary evaporators, with the possibility to be used outside the fume hood thanks to the integrated vapor evacuation system.

Video MultiVap 8

MultiVap 6

The MultiVap 6 is a basic, cost effective sample preparation instrument featuring 6 independent channels. Without transferring, samples are directly concentrated in the vial thereby reducing the risk of transfer loss, improving recovery and saving precious time. The nitrogen fl ow rate of each channel can be adjusted independently as well as the needles that can be adjusted up and down. The aluminum alloy heating block guarantees temperature uniformity for all samples.

9 good reasons to choose LabTech Concentrators

  • ●  Advanced solutions and features

    Enhanced productivity

  • ●  Vortex nitrogen purge

    Provides high speed evaporation

  • ●  10, 50 & 200 mL concentration cups can be accommodated

    Flexible and time saving

  • ●  Automatic adjustment of nitrogen needle according to the sample volume reduction

    Precision, repeatability and gas saving

  • ●  Sample cups do not need to be handled

    Time saving and error proof

  • ● Cups can go directly to the liquid handler of GPC/SPE

    Time saving

  • ● Visible concentration process

    Peace of mind

  • ●  Automatic detection of the liquid endpoint

    Error proof and unattended operations

  • ● Excellent price/performance ratio

    A solution for any budget

KOVALENTS PHILOSOPHY

At Kovalent we strive to establish and develop long-term partnerships with our customers. When we use the word “partnership” we mean that Kovalent becomes an integral part of our customers’ projects, where we are expected to fully understand their application needs. We aim to make Kovalent our customers’ first and natural choice, and this ambition permeates our entire organisation from initial contact to finished and delivered product.

CONTACTS:

Kovalent AB
Hulda Lindgrens gata 6A
421 31 VÄSTRA FRÖLUNDA
Sweden
Phone: +46(0)31-709 81 80
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Org.nr: 556417-2194

SKANDINAVISKA GENETEC AB

Genetec is a member of the same family of companies as Kovalent. Both companies share the same set of values and are in many ways integrated with each other. The main difference between the two companies are their products. Kovalent is involved in system solutions including instrumentation and Genetec is mainly working with consumables. The focus of Genetec is chromatography accessories. You are welcome to visit their state of the art web shop: genetec.se